Anti-MyoD1抗体(ab64159)
Key features and details
- Rabbit polyclonal to MyoD1
- Suitable for: Sandwich ELISA, WB
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-MyoD1抗体
参阅全部 MyoD1 一抗 -
描述
兔多克隆抗体to MyoD1 -
宿主
Rabbit -
经测试应用
适用于: Sandwich ELISA, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Cow, Pig, Rhesus monkey
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human MyoD1.
参阅Abcam的专有抗源政策 (Peptide available as ab66473.) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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sELISA pair antibody
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab64159于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use a concentration of 0.1 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [5.2F] to MyoD1 (ab16148). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [5.2F] to MyoD1 (ab16148) as Capture.
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| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa).
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| 说明 |
|---|
|
Sandwich ELISA
Use a concentration of 0.1 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [5.2F] to MyoD1 (ab16148). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [5.2F] to MyoD1 (ab16148) as Capture. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa). |
靶标
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功能
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins. -
序列相似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
翻译后修饰
Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
Ubiquitinated on the N-terminus; which is required for proteasomal degradation. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 281938 Cow
- Entrez Gene: 4654 Human
- Entrez Gene: 17927 Mouse
- Entrez Gene: 407604 Pig
- Omim: 159970 Human
- SwissProt: Q7YS82 Cow
- SwissProt: P15172 Human
- SwissProt: P10085 Mouse
see all -
别名
- bHLHc1 antibody
- Class C basic helix-loop-helix protein 1 antibody
- MYF 3 antibody
see all
图片
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Western blot - Anti-MyoD1 antibody (ab64159)All lanes : Anti-MyoD1 antibody (ab64159) at 1 µg/ml
Lane 1 : Skeletal Muscle (Mouse) Tissue Lysate at 20 µg
Lane 2 : Rh30 Whole Cell Lysate at 5 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 47 kDa (possible post-translational modification)
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab64159 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
ab64159 detects a band at 45 kDa, while this differs to its predicted molecular weight of 34kDa, the banding pattern observed is consistent with what has been described in the literature PMID:19352326.
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Sandwich ELISA - Anti-MyoD1 antibody (ab64159)Standard Curve for Myo-D; dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [5.2F] to MyoD1 (ab16148) at 5ug/ml and Detector Antibody Rabbit polyclonal to MyoD1 (ab64159) at 0.1ug/ml.
数据表及文件
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SDS download
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Datasheet download
文献 (31)
ab64159 被引用在 31 文献中.
- Jama A et al. Lipin1 plays complementary roles in myofibre stability and regeneration in dystrophic muscles. J Physiol 601:961-978 (2023). PubMed: 36715084
- Vo L et al. Cardiolipin metabolism regulates expression of muscle transcription factor MyoD1 and muscle development. J Biol Chem 299:102978 (2023). PubMed: 36739949
- Wang J et al. CircRIMKLB promotes myoblast proliferation and inhibits differentiation by sponging miR-29c to release KCNJ12. Epigenetics 17:1686-1700 (2022). PubMed: 35348434
- Ferrari R et al. Inflammatory Caspase Activity Mediates HMGB1 Release and Differentiation in Myoblasts Affected by Peripheral Arterial Disease. Cells 11:N/A (2022). PubMed: 35406727
- Sadeghmousavi S et al. The role of spinal cord tractography in detecting lesions following selective bladder afferent and efferent fibers injury: A novel method for induction of neurogenic lower urinary tract dysfunction in rabbit. Neurourol Urodyn 41:1539-1552 (2022). PubMed: 35842827