重组Anti-Myocilin抗体[EPR28799-15] (ab318197)
![Western blot - Anti-Myocilin antibody [EPR28799-15] (ab318197)](https://www.abcam.cn/ps/products/318/ab318197/Images/ab318197-542843-anti-myocilin-antibody-epr28799-15-western-blot-neuro-2a-mouse-neuroblastoma-neuroblast-whole-fresh-cell-lysate-20-ug.jpg "Western blot - Anti-Myocilin antibody [EPR28799-15] (ab318197)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR28799-15] to Myocilin
- Suitable for: ICC/IF, Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Myocilin抗体[EPR28799-15]
参阅全部 Myocilin 一抗 -
描述
兔单克隆抗体[EPR28799-15] to Myocilin -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, Flow Cyt (Intra), WBmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Neuro-2a whole cell, U-87 MG whole cell, SH-SY5Y whole cell, HeLa whole cell, MCF7 whole cell, C2C12 whole cell, Human eyeball tissue, Human testis tissue, Human spleen tissue, Human liver tissue, Mouse retina tissue, Mouse testis tissue, Rat retina tissue and Rat testis tissue lysates. ICC/IF: HeLa, mouse primary retina and rat primary retina cells. Flow Cyt (Intra): HeLa, Mouse retina and Rat retina cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR28799-15 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab318197于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/500.
|
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| Flow Cyt (Intra) |
1/50 - 1/500.
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| WB |
1/1000. Predicted molecular weight: 57 kDa.
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| 说明 |
|---|
|
ICC/IF
1/500. |
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Flow Cyt (Intra)
1/50 - 1/500. |
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WB
1/1000. Predicted molecular weight: 57 kDa. |
靶标
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功能
May participate in the obstruction of fluid outflow in the trabecular meshwork. -
组织特异性
Expressed in large amounts in various types of muscle, ciliary body, papillary sphincter, skeletal muscle, heart and other tissues. Expressed predominantly in the retina. In normal eyes, found in the inner uveal meshwork region and the anterior portion of the meshwork. In contrast, in many glaucomatous eyes, it is found in more regions of the meshwork and appeared more intensively than in normal eyes, regardless of the type or clinical severity of glaucoma. -
疾病相关
Defects in MYOC are the cause of primary open angle glaucoma type 1A (GLC1A) [MIM:137750]. Primary open angle glaucoma (POAG) is characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place.
Defects in MYOC may also contribute to primary congenital glaucoma type 3A (GLC3A) [MIM:231300]. Defects in MYOC may contribute to this phenotype via digenic inheritance. GLC3A is an autosomal recessive form of primary congenital glaucoma (PCG). PCG is characterized by marked increase of intraocular pressure at birth or early choldhood, large ocular globes (buphthalmos) and corneal edema. It results from developmental defects of the trabecular meshwork and anterior chamber angle of the eye that prevent adequate drainage of aqueous humor. -
序列相似性
Contains 1 olfactomedin-like domain. -
翻译后修饰
Different isoforms may arise by post-translational modifications.
Glycosylated.
Palmitoylated. -
细胞定位
Rough endoplasmic reticulum. Secreted. Cell projection > cilium. Located preferentially in the ciliary rootlet and basal body of the connecting cilium of photoreceptor cells, and in the rough endoplasmic reticulum. Also secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 4653 Human
- Entrez Gene: 17926 Mouse
- Entrez Gene: 81523 Rat
- Omim: 601652 Human
- SwissProt: Q99972 Human
- SwissProt: O70624 Mouse
- SwissProt: Q9R1J4 Rat
- Unigene: 436037 Human
see all -
别名
- GLC1A antibody
- GPOA antibody
- JOAG antibody
see all
图片
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Western blot - Anti-Myocilin antibody [EPR28799-15] (ab318197)All lanes : Anti-Myocilin antibody [EPR28799-15] (ab318197) at 1/1000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma neuroblast) whole fresh cell lysate
Lane 2 : U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole fresh cell lysate
Lane 3 : SH-SY5Y (human neuroblastoma epithelial cell) whole fresh cell lysate
Lane 4 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 5 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 6 : C2C12 (mouse myoblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 57 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The identity of the higher MW band at approximately 150kDa (Lane 3) is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Exposure time: Lane 1-3: 92 seconds; Lane 4-6: 180 seconds
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![Western blot - Anti-Myocilin antibody [EPR28799-15] (ab318197)](https://www.abcam.cn/ps/products/318/ab318197/Images/ab318197-542844-anti-myocilin-antibody-epr28799-15-western-blot-human-eyeball-tissue-lysate-20-ug.jpg)
Western blot - Anti-Myocilin antibody [EPR28799-15] (ab318197)All lanes : Anti-Myocilin antibody [EPR28799-15] (ab318197) at 1/1000 dilution
Lane 1 : Human eyeball tissue lysate
Lane 2 : Human testis tissue lysate
Lane 3 : Human spleen tissue lysate
Lane 4 : Human liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 57 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 103 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: Human spleen and liver (PMID: 10320784).
The bands beneath the target band (20~37 kDa) are likely to be degraded target fragments.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
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![Western blot - Anti-Myocilin antibody [EPR28799-15] (ab318197)](https://www.abcam.cn/ps/products/318/ab318197/Images/ab318197-542845-anti-myocilin-antibody-epr28799-15-western-blot-mouse-retina-tissue-lysate-20-ug.jpg)
Western blot - Anti-Myocilin antibody [EPR28799-15] (ab318197)All lanes : Anti-Myocilin antibody [EPR28799-15] (ab318197) at 1/1000 dilution
Lane 1 : Mouse retina tissue lysate
Lane 2 : Mouse testis tissue lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : Rat retina tissue lysate
Lane 5 : Rat testis tissue lysate
Lane 6 : Rat liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 57 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 92 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: Mouse and rat liver (PMID: 10320784).
The identity of the lower MW band at approximately 37kDa (Lane 3) is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
-
![Immunocytochemistry/ Immunofluorescence - Anti-Myocilin antibody [EPR28799-15] (ab318197)](https://www.abcam.cn/ps/products/318/ab318197/Images/ab318197-4-anti-myocilin-antibody-epr28799-15-immunocytochemistry-hela.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Myocilin antibody [EPR28799-15] (ab318197)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Myocilin with ab318197 at 1/500 (1.016 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor ® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing cytoplasmic staining in HeLa cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor ® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor ® 488) preadsorbed at 1/1000 2 ug/ml dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-Myocilin antibody [EPR28799-15] (ab318197)](https://www.abcam.cn/ps/products/318/ab318197/Images/ab318197-5-anti-myocilin-antibody-epr28799-15-immunocytochemistry-mouse-primary-retina.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Myocilin antibody [EPR28799-15] (ab318197)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary retina cells labelling Myocilin with ab318197 at 1/500 (1.016 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor ® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing cytoplasmic staining in mouse primary retina (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection.
The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
-ve control 1: ab318197 at 1/500 (1.016 ug/ml) dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 2 ug/mL dilution.
-ve control 2: ab11267 1/500 4ug/ml dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-Myocilin antibody [EPR28799-15] (ab318197)](https://www.abcam.cn/ps/products/318/ab318197/Images/ab318197-6-anti-myocilin-antibody-epr28799-15-immunocytochemistry-rat-primary-retina.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Myocilin antibody [EPR28799-15] (ab318197)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary retina cells labelling Myocilin with ab318197 at 1/500 (1.016 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor ® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing cytoplasmic staining in rat primary retina (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection.
The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
-ve control 1: ab318197 at 1/500 (1.016 ug/ml) dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 2 ug/mL dilution.
-ve control 2: ab11267 1/500 4ug/ml dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution. -
![Flow Cytometry (Intracellular) - Anti-Myocilin antibody [EPR28799-15] (ab318197)](https://www.abcam.cn/ps/products/318/ab318197/Images/ab318197-7-anti-myocilin-antibody-epr28799-15-flow-cytometry-hela.jpg)
Flow Cytometry (Intracellular) - Anti-Myocilin antibody [EPR28799-15] (ab318197)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Myocilin with ab318197 at 1/500 dilution (0.1ug) / Magenta compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor ® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
-
![Flow Cytometry (Intracellular) - Anti-Myocilin antibody [EPR28799-15] (ab318197)](https://www.abcam.cn/ps/products/318/ab318197/Images/ab318197-8-anti-myocilin-antibody-epr28799-15-flow-cytometry-mouse-retina.jpg)
Flow Cytometry (Intracellular) - Anti-Myocilin antibody [EPR28799-15] (ab318197)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse retina cells labelling Myocilin with ab318197 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Goat Anti-Rabbit IgG (Alexa Fluor ® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
-
![Flow Cytometry (Intracellular) - Anti-Myocilin antibody [EPR28799-15] (ab318197)](https://www.abcam.cn/ps/products/318/ab318197/Images/ab318197-9-anti-myocilin-antibody-epr28799-15-flow-cytometry-ratretina.jpg)
Flow Cytometry (Intracellular) - Anti-Myocilin antibody [EPR28799-15] (ab318197)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat retina cells labelling Myocilin with ab318197 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Goat Anti-Rabbit IgG (Alexa Fluor ® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
-
![Anti-Myocilin antibody [EPR28799-15] (ab318197)](https://www.abcam.cn/ps/products/318/ab318197/Images/ab318197-10-benefitsofrecombinantantibodies.jpg)
Anti-Myocilin antibody [EPR28799-15] (ab318197)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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