Anti-MYO9B抗体(ab52026)
Key features and details
- Goat polyclonal to MYO9B
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-MYO9B抗体
参阅全部 MYO9B 一抗 -
描述
山羊多克隆抗体to MYO9B -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide:
C-KNDFEPVKQSKAQ
, corresponding to internal sequence amino acids 1605-1617 of Human MYO9B -
阳性对照
- Human Liver lysate.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52026于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
| 说明 |
|---|
应用说明
Peptide ELISA: Antibody detection limit dilution 1:2,000.
WB: Use at a concentration of 2 - 6 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 244 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 2 - 6 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 244 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
-
功能
Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Binds actin with high affinity both in the absence and presence of ATP and its mechanochemical activity is inhibited by calcium ions (PubMed:9490638). Also acts as a GTPase activator for RHOA (PubMed:9490638, PubMed:26529257). Plays a role in the regulation of cell migration via its role as RHOA GTPase activator. This is regulated by its interaction with the SLIT2 receptor ROBO1; interaction with ROBO1 impairs interaction with RHOA and subsequent activation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA (PubMed:26529257). -
组织特异性
Detected in peripheral blood leukocytes (at protein level) (PubMed:9490638). Expressed predominantly in peripheral blood leukocytes and at lower levels, in thymus, spleen, testis, prostate, ovary, brain, small intestine and lung. -
疾病相关
Celiac disease 4 -
序列相似性
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.
Contains 4 IQ domains.
Contains 1 myosin motor domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Ras-associating domain.
Contains 1 Rho-GAP domain. -
细胞定位
Cytoplasm, cell cortex. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton. In undifferentiated cells colocalizes with F-actin in the cell periphery while in differentiated cells its localization is cytoplasmic with the highest levels in the perinuclear region. - Information by UniProt
-
数据库链接
- Entrez Gene: 4650 Human
- Omim: 602129 Human
- SwissProt: Q13459 Human
- Unigene: 123198 Human
-
别名
- CELIAC 4 antibody
- Celiac disease 4 antibody
- CELIAC4 antibody
see all
图片
-
Western blot - Anti-MYO9B antibody (ab52026)Anti-MYO9B antibody (ab52026) at 2 µg/ml + Human Liver lysate (35µg protein in RIPA buffer).
Predicted band size: 244 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
数据表及文件
-
Datasheet download
文献 (0)
ab52026 尚未被引用在任何文献中。
