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AB249795

重组Anti-MYO19抗体[EPR12551-13] - BSA and Azide free

Anti-MYO19 antibody [EPR12551-13] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal MYO19 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

查看别名

MYOHD1, MYO19, Unconventional myosin-XIX, Myosin head domain-containing protein 1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-MYO19 antibody [EPR12551-13] - BSA and Azide free (AB249795)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MYO19 antibody [EPR12551-13] - BSA and Azide free (AB249795)

This data was developed using ab174286, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of A549 cells labeling MYO19 with ab174286 at 1/50 (red). DAPI nuclear staining (blue).

Western blot - Anti-MYO19 antibody [EPR12551-13] - BSA and Azide free (AB249795)
  • WB

Supplier Data

Western blot - Anti-MYO19 antibody [EPR12551-13] - BSA and Azide free (AB249795)

This data was developed using ab174286, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MYO19 antibody [EPR12551-13] (<a href='/products/primary-antibodies/myo19-antibody-epr12551-13-ab174286'>ab174286</a>) at 1/1000 dilution

Lane 1:

HeLa cell line lysate at 10 µg

Lane 2:

A549 cell line lysate at 10 µg

Lane 3:

K562 cell line lysate at 10 µg

Lane 4:

293T cell line lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/500 dilution

Predicted band size: 109 kDa

true

不同偶联物与剂型 (1)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR12551-13

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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搭配使用产品

Sample Prep & Detection Kits

AB188286

Rhodamine Conjugation Kit (Fast) - Lightning-Link®

Schematic Diagram - Rhodamine Conjugation Kit (Fast) - Lightning-Link® (AB188286)

  • 0
  • 0
Sample Prep & Detection Kits

AB218260

Oligonucleotide Conjugation Kit

Conjugation - Oligonucleotide Conjugation Kit (AB218260)

  • 0
  • 0
Primary Antibodies

AB188029

Anti-MIRO1 antibody [CL1083]

KO Validated

Western blot - Anti-MIRO1 antibody [CL1083] (AB188029)

  • 4
  • 4
Primary Antibodies

AB320739

Anti-MIRO2 antibody [EPR29118-85]

BOND RX™ Validated|20ul selling size|KO Validated|Recombinant|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIRO2 antibody [EPR29118-85] (AB320739)

  • 0
  • 0
Primary Antibodies

AB110329

Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker

Lab Essentials

Western blot - Anti-Mitofilin antibody [2E4AD5] - Mitochondrial Marker (AB110329)

  • 5
  • 3
Primary Antibodies

AB167429

Anti-KIF5B+KIF5C antibody [EPR10276(B)]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-KIF5B+KIF5C antibody [EPR10276(B)] (AB167429)

  • 4
  • 3
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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产品详情

ab249795 is the carrier-free version of ab174286.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The MYO19 protein also known as Myosin XIX functions as an ATP-dependent motor protein. It possesses a molecular mass of approximately 119 kDa and is part of the myosin superfamily. This protein is known for its role in cellular processes requiring motility including vesicle and organelle transport. MYO19 is widely expressed in tissues with notable expression in the brain and skeletal muscles suggesting its importance in those regions.
Biological function summary

MYO19 contributes to mitochondrial dynamics by participating in mitochondrial motility and positioning within cells. While MYO19 is not part of a larger protein complex it interacts with the mitochondrial outer membrane and aids in tethering mitochondria to the cytoskeleton. This interaction is essential for maintaining proper cellular function specifically in energy-demanding tissues.

Pathways

MYO19 interacts with cellular transport mechanisms notably involving the actin cytoskeleton pathway. The protein facilitates mitochondrial movement along actin filaments. It shares pathways with other myosin family members such as MYO5 which are involved in intracellular transport. Additionally MYO19's role in actin-related pathways underlines its involvement in regulating cellular energy distribution and homeostasis.

MYO19 has potential links with neurodegenerative diseases and muscular disorders. Disruption in MYO19 function may lead to impaired mitochondrial distribution contributing to the pathology of conditions like amyotrophic lateral sclerosis (ALS). Additionally the protein shows possible connections with neurofilament-associated proteins suggesting a role in neural health. Such associations highlight the significance of MYO19 in understanding disease mechanisms and designing therapeutic strategies.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Actin-based motor molecule with ATPase activity that localizes to the mitochondrion outer membrane (PubMed : 19932026, PubMed : 23568824, PubMed : 25447992). Motor protein that moves towards the plus-end of actin filaments (By similarity). Required for mitochondrial inheritance during mitosis (PubMed : 25447992). May be involved in mitochondrial transport or positioning (PubMed : 23568824).
See full target information MYO19
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 9:775642 PubMed35004677

2021

HIF-1α Induces HECTD2 Up-Regulation and Aggravates the Malignant Progression of Renal Cell Cancer Repressing miR-320a.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Lv,Taimin Shen,Juncheng Yao,Qi Yang,Ying Xiang,Zhiwei Ma
View all publications
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Abcam Product Promise

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