Anti-MYL9抗体(ab64161)
Key features and details
- Rabbit polyclonal to MYL9
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-MYL9抗体
参阅全部 MYL9 一抗 -
描述
兔多克隆抗体to MYL9 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human, Recombinant fragment
预测可用于: Bird, Mammals
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免疫原
Synthetic peptide:
KKRPQRATSNVFAMFD
, corresponding to amino acids 12-27 of Human MYL9 -
阳性对照
- IHC-P: Human Smooth Muscle (colon).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab64161于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500 - 1/2000. Predicted molecular weight: 20 kDa.
|
| IHC-P |
Use a concentration of 2.5 µg/ml.
|
| 说明 |
|---|
|
WB
1/500 - 1/2000. Predicted molecular weight: 20 kDa. |
|
IHC-P
Use a concentration of 2.5 µg/ml. |
靶标
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功能
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. -
组织特异性
Smooth muscle tissues and in some, but not all, nonmuscle cells. -
序列相似性
Contains 3 EF-hand domains. -
翻译后修饰
Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. - Information by UniProt
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数据库链接
- Entrez Gene: 10398 Human
- Omim: 609905 Human
- SwissProt: P24844 Human
- Unigene: 504687 Human
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别名
- 20 kDa myosin light chain antibody
- Human 20kDa myosin light chain (MLC2) mRNA complete cds antibody
- LC20 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYL9 antibody (ab64161)
ab64161 at 2.5µg/ml staining MYL9 in Colon, Human Smooth Muscle by Immunohistochemistry, Formalin-fixed, paraffin-embedded tissue.
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Western blot - Anti-MYL9 antibody (ab64161)
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab64161 被引用在 5 文献中.
- Zhu H et al. Identification of the hub genes associated with prostate cancer tumorigenesis. Front Oncol 13:1168772 (2023). PubMed: 37251946
- Zhu K et al. Long non-coding RNA MBNL1-AS1 regulates proliferation, migration, and invasion of cancer stem cells in colon cancer by interacting with MYL9 via sponging microRNA-412-3p. Clin Res Hepatol Gastroenterol N/A:N/A (2019). PubMed: 31255531
- Chao CM et al. Impact of Fgf10 deficiency on pulmonary vasculature formation in a mouse model of bronchopulmonary dysplasia. Hum Mol Genet 28:1429-1444 (2019). PubMed: 30566624
- Xie X & Percipalle P Elevated transforming growth factor ß signaling activation in ß-actin-knockout mouse embryonic fibroblasts enhances myofibroblast features. J Cell Physiol N/A:N/A (2018). PubMed: 29851084
- Hudson CA et al. Phasic contractions of isolated human myometrium are associated with Rho-kinase (ROCK)-dependent phosphorylation of myosin phosphatase-targeting subunit (MYPT1). Mol Hum Reprod 18:265-79 (2012). WB . PubMed: 22155728