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重组RabMAb

重组Anti-Myeloperoxidase抗体[EPR4793] - BSA and Azide free (ab226155)

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Western blot - Anti-Myeloperoxidase antibody [EPR4793] - BSA and Azide free (ab226155)
  • Western blot - Anti-Myeloperoxidase antibody [EPR4793] - BSA and Azide free (ab226155)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4793] - BSA and Azide free (ab226155)
  • Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR4793] - BSA and Azide free (ab226155)
  • Anti-Myeloperoxidase antibody [EPR4793] - BSA and Azide free (ab226155)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4793] to Myeloperoxidase - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase APC HRP PE Unconjugated

概述

  • 产品名称

    Anti-Myeloperoxidase抗体[EPR4793] - BSA and Azide free
    参阅全部 Myeloperoxidase 一抗

  • 描述

    兔单克隆抗体[EPR4793] to Myeloperoxidase - BSA and Azide free

  • 宿主

    Rabbit

  • 特异性

    The antibody recognizes Myeloperoxidase (85-90 kDa) and its heavy chain (60 kDa).

  • 经测试应用

    适用于: WB, IHC-P, Flow Cyt (Intra)more details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Flow Cyt (intra): HL-60 cells

  • 常规说明

    ab226155 is the carrier-free version of ab134132.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab226155于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 83 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt (Intra)
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 83 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt (Intra)
Use at an assay dependent concentration.

靶标

  • 功能

    Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.

  • 疾病相关

    Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.

  • 序列相似性

    Belongs to the peroxidase family. XPO subfamily.

  • 细胞定位

    Lysosome.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 84 kDa myeloperoxidase antibody
      • 89 kDa myeloperoxidase antibody
      • EC 1.11.1.7 antibody
      • EC1.11.2.2 antibody
      • fj80f04 antibody
      • MPO antibody
      • mpx antibody
      • myeloid-specific peroxidase antibody
      • Myeloperoxidase antibody
      • Myeloperoxidase heavy chain antibody
      • Myeloperoxidase light chain antibody
      • PERM_HUMAN antibody
      • wu:fj80f04 antibody
      see all

    图片

    • Western blot - Anti-Myeloperoxidase antibody [EPR4793] - BSA and Azide free (ab226155)
      Western blot - Anti-Myeloperoxidase antibody [EPR4793] - BSA and Azide free (ab226155)
      Anti-Myeloperoxidase antibody [EPR4793] (ab134132) at 1/1000 dilution (Purified) + HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysate

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 83 kDa

    • Western blot - Anti-Myeloperoxidase antibody [EPR4793] - BSA and Azide free (ab226155)
      Western blot - Anti-Myeloperoxidase antibody [EPR4793] - BSA and Azide free (ab226155)
      Anti-Myeloperoxidase antibody [EPR4793] (ab134132) at 1/1000 dilution (Purified) + Human spleen lysate

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 83 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4793] - BSA and Azide free (ab226155)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4793] - BSA and Azide free (ab226155)

      This data was developed using ab134132, the same antibody clone in a different buffer formulation.
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling Myeloperoxidase with Purified ab134132 at 1:1000 dilution (0.2 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0) . Tissue was counterstained with Hematoxylin. LeicaDS9800 (Bond™ Polymer Refine Detection) was used secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. 

    • Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR4793] - BSA and Azide free (ab226155)
      Flow Cytometry (Intracellular) - Anti-Myeloperoxidase antibody [EPR4793] - BSA and Azide free (ab226155)

      This data was developed using ab134132, the same antibody clone in a different buffer formulation.

      Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell, Left) / HL-60 (Human acute promyelocytic leukemia promyeloblast, Right) cells labeling Myeloperoxidase with purified ab134132 at 1/10000 dilution (0.1 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
      Negative control: HeLa.

    • Anti-Myeloperoxidase antibody [EPR4793] - BSA and Azide free (ab226155)
      Anti-Myeloperoxidase antibody [EPR4793] - BSA and Azide free (ab226155)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab226155？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab226155 尚未被引用在任何文献中。

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