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重组RabMAb

重组Anti-Myeloperoxidase抗体[EPR4792] (ab109116)

Submit a review Submit a question References (2)
Western blot - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)
  • Anti-Myeloperoxidase antibody [EPR4792] (ab109116)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4792] to Myeloperoxidase
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Myeloperoxidase抗体[EPR4792]
    参阅全部 Myeloperoxidase 一抗

  • 描述

    兔单克隆抗体[EPR4792] to Myeloperoxidase

  • 宿主

    Rabbit

  • 特异性

    ab109116 recognizes Myeloperoxidase (85-90 kDa) and its light chain (15 kDa).

  • 经测试应用

    适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt or IP

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HL-60 cell lysate IHC-P: Human colon, lung and spleen tissues

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab109116于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Predicted molecular weight: 84 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.
说明
WB
1/1000 - 1/10000. Predicted molecular weight: 84 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.
应用说明
Is unsuitable for Flow Cyt or IP.

靶标

  • 功能

    Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.

  • 疾病相关

    Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.

  • 序列相似性

    Belongs to the peroxidase family. XPO subfamily.

  • 细胞定位

    Lysosome.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 84 kDa myeloperoxidase antibody
      • 89 kDa myeloperoxidase antibody
      • EC 1.11.1.7 antibody
      • EC1.11.2.2 antibody
      • fj80f04 antibody
      • MPO antibody
      • mpx antibody
      • myeloid-specific peroxidase antibody
      • Myeloperoxidase antibody
      • Myeloperoxidase heavy chain antibody
      • Myeloperoxidase light chain antibody
      • PERM_HUMAN antibody
      • wu:fj80f04 antibody
      see all

    图片

    • Western blot - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)
      Western blot - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)
      Anti-Myeloperoxidase antibody [EPR4792] (ab109116) at 1/1000 dilution + HL-60 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 84 kDa
      Observed band size: 85-90 kDa why is the actual band size different from the predicted?
      Additional bands at: 15 kDa (possible cleavage fragment)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)

      ab109116, at a 1/250 dilution, staining Myeloperoxidase in paraffin embedded Human colon tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)

      ab109116, at a 1/250 dilution, staining Myeloperoxidase in paraffin embedded Human lung tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)

      ab109116, at a 1/250 dilution, staining Myeloperoxidase in paraffin embedded Human spleen tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)

      ab109116 showing positive staining in Normal liver tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)

      ab109116 showing positive staining in Bone marrow tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myeloperoxidase antibody [EPR4792] (ab109116)

      ab109116 showing negative staining in Skeletal muscle tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Myeloperoxidase antibody [EPR4792] (ab109116)
      Anti-Myeloperoxidase antibody [EPR4792] (ab109116)

    文献 (2)

    发表研究结果有使用 ab109116？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab109116 被引用在 2 文献中.

    • Khamwong M  et al. Burkholderia pseudomallei biofilm phenotypes confined but surviving in neutrophil extracellular traps of varying appearance. Front Immunol 13:926788 (2022). PubMed: 36059509
    • Baek JH  et al. Extracellular Hb enhances cardiac toxicity in endotoxemic guinea pigs: protective role of haptoglobin. Toxins (Basel) 6:1244-59 (2014). WB, ICC/IF ; Guinea pig . PubMed: 24691127

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