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Immunology Innate Immunity Macrophage / Inflamm.

Anti-Myeloperoxidase抗体[2C7] (ab25989)

  • Datasheet
  • SDS
Reviews (5)Q&A (5)References (93)

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Flow Cytometry - Anti-Myeloperoxidase antibody [2C7] (ab25989)

    Key features and details

    • Mouse monoclonal [2C7] to Myeloperoxidase
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    FITC PE

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    概述

    • 产品名称

      Anti-Myeloperoxidase抗体[2C7]
      参阅全部 Myeloperoxidase 一抗
    • 描述

      小鼠单克隆抗体[2C7] to Myeloperoxidase
    • 宿主

      Mouse
    • 经测试应用

      适用于: Flow Cytmore details
    • 种属反应性

      与反应: Human
    • 免疫原

      Full length native protein (purified) corresponding to Human Myeloperoxidase.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • 存储溶液

      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • 纯度

      Protein A purified
    • 克隆

      单克隆
    • 克隆编号

      2C7
    • 骨髓瘤

      x63-Ag8.653
    • 同种型

      IgG1
    • 研究领域

      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Cardiovascular
      • Blood
      • Other
      • Cardiovascular
      • Vasculature
      • Vasculature Markers
      • Arterial
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      • Redox metabolism
      • Antioxidants
      • Metabolism
      • Types of disease
      • Cancer

    相关产品

    • Alternative Versions

      • FITC Anti-Myeloperoxidase antibody [2C7] (ab11729)
      • PE Anti-Myeloperoxidase antibody [2C7] (ab11730)
    • Assay kits

      • Myeloperoxidase (MPO) Activity Assay Kit (Colorimetric) (ab105136)
      • Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) (ab111749)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
      • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab25989于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Flow Cyt
    1/20 - 1/100.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    说明
    Flow Cyt
    1/20 - 1/100.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    靶标

    • 功能

      Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
    • 疾病相关

      Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
    • 序列相似性

      Belongs to the peroxidase family. XPO subfamily.
    • 细胞定位

      Lysosome.
    • Target information above from: UniProt accession P05164 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 4353 Human
      • Omim: 606989 Human
      • SwissProt: P05164 Human
      • Unigene: 458272 Human
      • 别名

        • 84 kDa myeloperoxidase antibody
        • 89 kDa myeloperoxidase antibody
        • EC 1.11.1.7 antibody
        • EC1.11.2.2 antibody
        • fj80f04 antibody
        • MPO antibody
        • mpx antibody
        • myeloid-specific peroxidase antibody
        • Myeloperoxidase antibody
        • Myeloperoxidase heavy chain antibody
        • Myeloperoxidase light chain antibody
        • PERM_HUMAN antibody
        • wu:fj80f04 antibody
        see all

      图片

      • Flow Cytometry - Anti-Myeloperoxidase antibody [2C7] (ab25989)
        Flow Cytometry - Anti-Myeloperoxidase antibody [2C7] (ab25989)
        Overlay histogram showing HeLa cells stained with ab25989 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab25989, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

      实验方案

      • Flow cytometry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (93)

      发表研究结果有使用 ab25989?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab25989 被引用在 93 文献中.

      • Miao N  et al. Oxidized mitochondrial DNA induces gasdermin D oligomerization in systemic lupus erythematosus. Nat Commun 14:872 (2023). PubMed: 36797275
      • Hallberg LAE  et al. Antioxidant Strategies to Modulate NETosis and the Release of Neutrophil Extracellular Traps during Chronic Inflammation. Antioxidants (Basel) 12:N/A (2023). PubMed: 36830036
      • Leal VNC  et al. Severe COVID-19 patients show a dysregulation of the NLRP3 inflammasome in circulating neutrophils. Scand J Immunol 97:e13247 (2023). PubMed: 36541819
      • Xu Q  et al. High expression of P-selectin induces neutrophil extracellular traps via the PSGL-1/Syk/Ca2+/PAD4 pathway to exacerbate acute pancreatitis. Front Immunol 14:1265344 (2023). PubMed: 37841279
      • Li X  et al. FGL2-MCOLN3-Autophagy Axis-Triggered Neutrophil Extracellular Traps Exacerbate Liver Injury in Fulminant Viral Hepatitis. Cell Mol Gastroenterol Hepatol 14:1077-1101 (2022). PubMed: 35926777
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-10 of 10 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Myeloperoxidase antibody [2C7]

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Rat Cell (BALF neutrophils from Rat)
      Permeabilization
      Yes - Triton
      Specification
      BALF neutrophils from Rat
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
      Fixative
      Paraformaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Feb 25 2021

      ELISA abreview for Anti-Myeloperoxidase antibody [2C7]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      ELISA
      Sample
      Human Purified protein (Natural human Myeloperoxidase protein)
      Specification
      Natural human Myeloperoxidase protein
      Blocking step
      BSA as blocking agent for 15 minute(s) · Concentration: 0.5% · Temperature: 24°C
      Type
      Other
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Manuel Gutiérrez-Capitán

      Verified customer

      提交于 Dec 03 2018

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Myeloperoxidase antibody [2C7]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (LIVER)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Ph6 (ER1) 20 MINS
      Permeabilization
      No
      Specification
      LIVER
      Blocking step
      H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 21°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      MR. Christopher Bagnall

      Verified customer

      提交于 Nov 22 2016

      Western blot abreview for Anti-Myeloperoxidase antibody [2C7]

      Poor
      Abreviews
      Abreviews
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (ventricular tissue lysates)
      Loading amount
      50 µg
      Specification
      ventricular tissue lysates
      Gel Running Conditions
      Non-reduced Denaturing (4~12% gradient NuPAGE)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Jul 05 2008

      Western blot abreview for Anti-Myeloperoxidase antibody [2C7]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Tissue lysate - whole (human aortic tissue extract)
      Loading amount
      30 µg
      Specification
      human aortic tissue extract
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Guo-Ping Shi

      Verified customer

      提交于 Mar 03 2008

      Question

      Inquiry: I am trying to create an MPO-DNA sandwich ELISA for immune complexes and I have been unsuccessful so far. I have been using a mouse polyclonal anti-human MPO antibody to coat, but I think this is the problem. I am interested in your monoclonal MPO Ab and think it might work but I can't afford to buy a full tube and have it not work for my experiment. I was wondering if there is any way you can send a small aliquot to test one of my samples? If it works then I would be interested in purchasing. I hope you understand and can help in some way. Thank you.

      Read More

      Abcam community

      Verified customer

      Asked on Dec 05 2012

      Answer

      Thank you for contacting us. Because we carry over 100,000 products, it isn't feasible for us to keep small sample sizes of our products.

      We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

      For MPO, we have tested a pair of antibodies in sandwich ELISA and guarantee them to work in human samples. The 2 are listed below:
      capture: ab10165 (https://www.abcam.com/Myeloperoxidase-antibody-4A4-ab10165.html)
      detection: ab24467 (https://www.abcam.com/Myeloperoxidase-antibody-2A11-HRP-ab24467.html)

      Since sandwich ELISA's can be hit or miss with antibody pairing since each antibody needs to have a unique antigen that it recognizes, going with the pair above is what I recommend since we can guarantee they will work together while we do not guarantee that ab25989 will work with your other antibody. That way, if ab10165 and ab24467 do not work for you in sELISA and we cannot work with you to get them to work we will refund your purchase or send you replacements free of charge.

      I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

      Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

      Read More

      Abcam Scientific Support

      回复于 Dec 05 2012

      Question

      Dear Sir/Madam, We prefer the monoclonal antibody because we stain skin sections. We have experienced that polyclonal antibodies tend to give more background in skin sections. Furthermore we also want to try to perform a double-stain with MPO against other anti-bodies. Therefore we prefer the monoclonal variant (ab25989). So we would like an issue discount for this antbody.  Kind regards,  

      Read More

      Abcam community

      Verified customer

      Asked on Dec 19 2011

      Answer

      DISCOUNT CODE: xxxxxxx Expiration date: 2012-04-20 As discussed I have generated a discount code for ab25989. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for staining of pig samples and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount

      Read More

      Abcam Scientific Support

      回复于 Dec 19 2011

      Question

      Dear Sir/Madam, We are planning to test the antibody against Anti-Myeloperoxidase (MPO) ab25989 on paraffin sections of pig samples in order to write an Abreview about it. Can you please inform us if this is still possible and how we can submit our review. Kind regards,  

      Read More

      Abcam community

      Verified customer

      Asked on Dec 19 2011

      Answer

      Thank you for contacting us and considering our testing discount with ab25989. As the sequence of human Myeloperoxidase shares 86% homology with the pig protein we would predict that  ab25989 would be able to recognise this protein. I can issue a testing discount against this antibody, or if you would like you could choose from the other antibodies we have available against Myeloperoxidase such as rabbit polyclonals ab45977 and ab9535 which have been shown to work well in paraffin embedded tissue sections. More information on the testing dicount can be found here: www.abcam.com/collaborationdiscount If you would like me to issue a testing discount for any of these antibodies please let me know. Once issues, this discount code can be activated once you have submitted an Abreview. More information on how to do this can be found from the following link: www.abcam.com/abreviews We publish both negative and positive Abreviews so your code will be activated regardless of the results of your tests. Once activated you can use your discount code to purchase any primary antibody of your choice from our catalogue free of charge. If you need any further information please do not hesitate to let me know, otherwise I look forward to hearing which antibody you would like to test.

      Read More

      Abcam Scientific Support

      回复于 Dec 19 2011

      Question

      I have an inquiry about your monoclonal myeloperoxidase (MPO) antibodies in general. We are going to be looking for possible MPO fusion proteins, most likely by looking for changes in MPO protein size by western blot. For this we would like a N-terminal MPO antibody. The only information that I could find about the epitope location for your MPO antibodies was that one is located in the heavy chain, which I believe starts at amino acid 279. What is the location of this epitope and do you have any antibodies that are more N-terminal than this one?

      Read More

      Abcam community

      Verified customer

      Asked on Jun 20 2007

      Answer

      Thank you for your enquiry regarding ab25989 and your interest in our products. The immunogen was full-length myeloperoxidase. The antibody recognizes an epitope on the heavy chain, but the exact epitope location has not been mapped.

      Read More

      Abcam Scientific Support

      回复于 Jun 20 2007

      Question

      Will this antibody detect a band at 84 kDa or at 150 kDa (Native Myeloperoxidase is represented as a covalently bound tetrameric complex of two glycosylated alpha chains (MW 59 - 64 kDa) and two unglycosylated beta chains (MW 14 kDa) with total MW 150 kDa)?

      Read More

      Abcam community

      Verified customer

      Asked on Apr 26 2006

      Answer

      Ab25989 recognizes an epitope on the heavy chain of myeloperoxidase. The protein is a multimer of 2 heavy chains (55kDa) and two light chains (15kDa), the heavy chains being linked by a disulfide bond. In Western blot under non-reducing conditions, the antibody recognizes native MPO (whole molecule) and will recognize the heavy chain following boiling of the sample. Please contact us again if you have any additional questions.

      Read More

      Abcam Scientific Support

      回复于 Apr 27 2006

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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