Anti-Myelin PLP 抗体 [plpc 1]

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Myelin PLP antibody [plpc 1] (AB9311)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling Myelin PLP with ab9311 at 1/25 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1 : 1000 dilution (Green). is observed. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1 : 1000 dilution.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [plpc 1] (AB9311)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling Myelin PLP with ab9311 at 1/5000 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use. Positive staining on human cerebrum is observed. Counter stained with Hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use.

The section was incubated with ab9311 for 30 mins at room temperature and followed by mouse specific IgG antibody for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [plpc 1] (AB9311)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labelling Myelin PLP with ab9311 at 1/5000 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use. Positive staining on human cerebrum is observed. Counter stained with Hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use.

The section was incubated with ab9311 for 30 mins at room temperature and followed by mouse specific IgG antibody for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [plpc 1] (AB9311)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat cerebellum tissue labeling Myelin PLP with ab9311 at 1 : 25 (32.36 μg/ml) dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) secondary at 1 : 1000 dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody was ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1 : 1000 dilution.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [plpc 1] (AB9311)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse cerebellum tissue labeling Myelin PLP with ab9311 at 1 : 25 (32.36 μg/ml) dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) secondary at 1 : 1000 dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody was ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1 : 1000 dilution.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin PLP antibody [plpc 1] (AB9311)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labelling Myelin PLP with ab9311 at 1/5000 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use. Positive staining on human cerebrum is observed. Counter stained with Hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use.

The section was incubated with ab9311 for 30 mins at room temperature and followed by mouse specific IgG antibody for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [plpc 1] (AB9311)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat cerebellum tissue labeling Myelin PLP with ab9311 at 1 : 25 (32.36 μg/ml) dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) secondary at 1 : 1000 dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody was ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1 : 1000 dilution.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Myelin PLP antibody [plpc 1] (AB9311)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse cerebellum tissue labeling Myelin PLP with ab9311 at 1 : 25 (32.36 μg/ml) dilution followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) secondary at 1 : 1000 dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody was ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1 : 1000 dilution.

• WB

Lab

Western blot - Anti-Myelin PLP antibody [plpc 1] (AB9311)

Samples are non-boiled as boiling may cause protein aggregates.

The molecular weight observed is consistent with what has been described in the literature (PMID : 9247276).

Negative control : liver (PMID : 2414013).

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Myelin PLP antibody [plpc 1] (ab9311) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 40 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

false

Exposure time: 3min