重组Anti-Myelin PLP抗体[EPR12675(2)(B)] (ab183493)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12675(2)(B)] to Myelin PLP
- Suitable for: IHC-P, IP
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Myelin PLP抗体[EPR12675(2)(B)]
参阅全部 Myelin PLP 一抗 -
描述
兔单克隆抗体[EPR12675(2)(B)] to Myelin PLP -
宿主
Rabbit -
经测试应用
适用于: IHC-P, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human and Mouse brain tissues; Mouse brain lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12675(2)(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-Myelin PLP antibody [EPR12675(2)(B)] - BSA and Azide free (ab250654)
- Alexa Fluor® 488 Anti-Myelin PLP antibody [EPR12675(2)(B)] (ab320202)
- Alexa Fluor® 647 Anti-Myelin PLP antibody [EPR12675(2)(B)] (ab320203)
- Alexa Fluor® 555 Anti-Myelin PLP antibody [EPR12675(2)(B)] (ab320204)
- Alexa Fluor® 594 Anti-Myelin PLP antibody [EPR12675(2)(B)] (ab320205)
- PE Anti-Myelin PLP antibody [EPR12675(2)(B)] (ab320206)
- APC Anti-Myelin PLP antibody [EPR12675(2)(B)] (ab320207)
- Alexa Fluor® 750 Anti-Myelin PLP antibody [EPR12675(2)(B)] (ab321277)
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183493于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (2) |
1/1000 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
IP |
1/30 - 1/50.
|
说明 |
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IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/30 - 1/50. |
靶标
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功能
This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin. -
疾病相关
Defects in PLP1 are the cause of leukodystrophy hypomyelinating type 1 (HLD1) [MIM:312080]; also known as Pelizaeus-Merzbacher disease. HLD1 is an X-linked recessive dysmyelinating disorder of the central nervous system in which myelin is not formed properly. It is characterized clinically by nystagmus, spastic quadriplegia, ataxia, and developmental delay.
Defects in PLP1 are the cause of spastic paraplegia X-linked type 2 (SPG2) [MIM:312920]. SPG2 is characterized by spastic gait and hyperreflexia. In some patients, complicating features include nystagmus, dysarthria, sensory disturbance, mental retardation, optic atrophy. -
序列相似性
Belongs to the myelin proteolipid protein family. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5354 Human
- Entrez Gene: 18823 Mouse
- Entrez Gene: 24943 Rat
- Omim: 300401 Human
- SwissProt: P60201 Human
- SwissProt: P60202 Mouse
- SwissProt: P60203 Rat
- Unigene: 1787 Human
see all -
别名
- HLD1 antibody
- Lipophilin antibody
- Major myelin proteolipid protein antibody
see all
图片
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Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Myelin PLP with ab183493 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling Myelin PLP with ab183493 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Western blot analysis of Myelin PLP in Mouse brain lysate immunoprecipitated using ab183493 at 1/50 dilution (Lane 1). Lane 2: TBS instead of mouse brain lysate.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (2)
ab183493 被引用在 2 文献中.
- Hagan N et al. CSF1R signaling is a regulator of pathogenesis in progressive MS. Cell Death Dis 11:904 (2020). PubMed: 33097690
- Cardona C et al. Identification of the iduronate-2-sulfatase proteome in wild-type mouse brain. Heliyon 5:e01667 (2019). PubMed: 31193135