重组Anti-Myelin oligodendrocyte glycoprotein抗体[EP4281] (ab109746)

Blocking buffer concentrations: 5% NFDM/TBST

Diluting buffer concentrations: 5% NFDM/TBST

Exposure equipment: iBright CL 1000 Imaging System

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The bands beneath the target band (25 kDa) are likely to be degraded target fragments.

In lanes 1-2, to minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In lanes 3-4, this blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

Exposure time:

Lanes 1-2: 180 seconds

Lanes 3-4: 92 seconds

Exposure equipment: iBright CL 1000 Imaging System.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling Myelin oligodendrocyte glycoprotein with purified ab109746 at 1:1000 dilution (1.12 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling Myelin oligodendrocyte glycoprotein with Purified ab109746 at 1:1000 dilution (1.12 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Myelin oligodendrocyte glycoprotein with purified ab109746 at 1:1000 dilution (1.12 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human astrocytoma tissue sections labeling Myelin oligodendrocyte glycoprotein with purified ab109746 at 1:1000 dilution (1.12 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.

ab109746 showing negative staining in Breast carcinoma tissue. This image was produced using unpurified antibody.

ab109746 showing negative staining in Normal breast tissue. This image was produced using unpurified antibody.

ab109746 showing negative staining in Colonic adenocarcinoma tissue. This image was produced using unpurified antibody.

ab109746 showing negative staining in Normal tonsil tissue. This image was produced using unpurified antibody.

ab109746 showing negative staining in Normal colon tissue. This image was produced using unpurified antibody.

ab109746 showing negative staining in Benign prostatic hyperplasia tissue. This image was produced using unpurified antibody.