重组Anti-Myelin Basic蛋白抗体[EPR6652] (ab133620)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6652] to Myelin Basic Protein
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Myelin Basic蛋白抗体[EPR6652]
参阅全部 Myelin Basic Protein 一抗 -
描述
兔单克隆抗体[EPR6652] to Myelin Basic蛋白 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Human -
免疫原
Recombinant fragment within Human Myelin Basic Protein aa 100 to the C-terminus. The exact sequence is proprietary.
Database link: P02686-1 -
阳性对照
- Human fetal brain lysate, Myelin Basic Protein recombinant protein, Human oligodendroglioma and Human brain tissues
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6652 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133620于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000 - 1/50000. Detects a band of approximately 19 kDa (predicted molecular weight: 33 kDa).
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IHC-P | (2) |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
1/10000 - 1/50000. Detects a band of approximately 19 kDa (predicted molecular weight: 33 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. -
组织特异性
MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. -
疾病相关
Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease). -
序列相似性
Belongs to the myelin basic protein family. -
发展阶段
Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter. -
翻译后修饰
Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated. -
细胞定位
Myelin membrane. Cytoplasmic side of myelin. - Information by UniProt
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数据库链接
- Entrez Gene: 4155 Human
- Omim: 159430 Human
- SwissProt: P02686 Human
- Unigene: 551713 Human
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别名
- GDB antibody
- Golli MBP antibody
- Golli MBP; myelin basic protein antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
Immunohistochemical analysis of paraffin embedded Human brain tissue labelling Myelin Basic Protein with ab133620 antibody at a dilution of 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
ab133620 showing positive staining in Astrocytoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Anti-Myelin Basic Protein antibody [EPR6652] (ab133620) at 1/10000 dilution + Human fetal brain lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 33 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
Immunohistochemical analysis of paraffin embedded Human oligodendroglioma tissue labelling Myelin Basic Protein with ab133620 antibody at a dilution of 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Anti-Myelin Basic Protein antibody [EPR6652] (ab133620) at 1/10000 dilution + Myelin Basic Protein recombinant protein at 0.01 µg
Predicted band size: 33 kDa
Note: the molecular weight of the recombinant protein is 46 kDa. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
ab133620 showing negative staining in Skeletal muscle tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620)
ab133620 showing negative staining in Normal kidney tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab133620 被引用在 5 文献中.
- Woelfle S et al. CLARITY increases sensitivity and specificity of fluorescence immunostaining in long-term archived human brain tissue. BMC Biol 21:113 (2023). PubMed: 37221592
- Li S et al. Ameliorative effects of Fingolimod (FTY720) on microglial activation and psychosis-related behavior in short term cuprizone exposed mice. Mol Brain 16:59 (2023). PubMed: 37438826
- Luo L et al. Application of bioactive hydrogels combined with dental pulp stem cells for the repair of large gap peripheral nerve injuries. Bioact Mater 6:638-654 (2021). PubMed: 33005828
- Sharma AD et al. Engineering a 3D functional human peripheral nerve in vitro using the Nerve-on-a-Chip platform. Sci Rep 9:8921 (2019). PubMed: 31222141
- Naudí A et al. Region-specific vulnerability to lipid peroxidation and evidence of neuronal mechanisms for polyunsaturated fatty acid biosynthesis in the healthy adult human central nervous system. Biochim Biophys Acta 1862:485-495 (2017). WB ; Human . PubMed: 28185952