JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB133620

重组Anti-Myelin Basic蛋白抗体[EPR6652]

Anti-Myelin Basic Protein antibody [EPR6652]

5

(5 Reviews)

|

(5 Publications)

Rabbit Recombinant Monoclonal Myelin Basic Protein antibody. Suitable for IHC-P, WB and reacts with Human, Recombinant full length protein samples. Cited in 5 publications.

查看别名

Myelin basic protein, MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (AB133620)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (AB133620)

ab133620 showing negative staining in Skeletal muscle tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (AB133620)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (AB133620)

Immunohistochemical analysis of paraffin embedded Human oligodendroglioma tissue labelling Myelin Basic Protein with ab133620 antibody at a dilution of 1/250.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (AB133620)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (AB133620)

ab133620 showing negative staining in Normal kidney tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (AB133620)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (AB133620)

ab133620 showing positive staining in Astrocytoma tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (AB133620)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [EPR6652] (AB133620)

Immunohistochemical analysis of paraffin embedded Human brain tissue labelling Myelin Basic Protein with ab133620 antibody at a dilution of 1/250.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-Myelin Basic Protein antibody [EPR6652] (AB133620)
  • WB

Unknown

Western blot - Anti-Myelin Basic Protein antibody [EPR6652] (AB133620)

All lanes:

Western blot - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620) at 1/10000 dilution

All lanes:

Human fetal brain lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 33 kDa

Observed band size: 19 kDa

false

Western blot - Anti-Myelin Basic Protein antibody [EPR6652] (AB133620)
  • WB

Unknown

Western blot - Anti-Myelin Basic Protein antibody [EPR6652] (AB133620)

Note : the molecular weight of the recombinant protein is 46 kDa.

All lanes:

Western blot - Anti-Myelin Basic Protein antibody [EPR6652] (ab133620) at 1/10000 dilution

All lanes:

Myelin Basic Protein recombinant protein at 0.01 µg

Predicted band size: 33 kDa

false

不同偶联物与剂型 (1)

  • Carrier free

    Anti-Myelin Basic Protein antibody [EPR6652] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR6652

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" }, "Recombinant full length protein": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" } } }
style
left-column

您可能对以下产品感兴趣:

AB150675

Luxol Fast Blue Stain Kit (Myelin Stain)

3

3 Reviews

查看产品
为什么推荐这个？

我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

style
left-column

产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
储存信息
Stable for 12 months at -20°C
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The myelin basic protein also referred to as MBP or MBP protein plays an important mechanical role in the central nervous system. It is a major component in the formation and stability of the myelin sheath which wraps around nerve fibers to ensure efficient neural signaling. The molecular weight of MBP can vary due to alternative splicing; one common form has a mass of about 18.5 kDa. MBP is expressed predominantly in oligodendrocytes within the brain and spinal cord where it helps form the myelin sheath. MBP2 is another variant that may be mentioned in some contexts.
Biological function summary

MBP is not just a passive structural element; it binds and interacts with lipids and other proteins to maintain the compact structure of the myelin sheath. MBP forms part of a complex that includes various other proteins and lipids ensuring the stability and function of myelin. Misfolding or altered expression of MBP can compromise myelin integrity affecting the insulation of neural pathways and signal transmission.

Pathways

MBP is an essential component in the pathways of myelination and neural plasticity. It plays a role in the signaling pathways that stimulate the development and maintenance of the myelin sheath. The protein interacts with other elements of the myelin sheath such as proteolipid protein (PLP) and myelin-associated glycoprotein (MAG) to coordinate its formation and repair processes.

MBP is linked closely to multiple sclerosis (MS) and other demyelinating conditions. Damage to or autoimmunity against MBP leads to the degradation of the myelin sheath contributing to the neurological symptoms in MS. The protein interacts with elements of the immune system with autoantibodies targeting MBP often found in MS patients. Another disorder associated with MBP dysfunction is Charcot-Marie-Tooth disease where abnormal MBP expression and structure can impact nerve function.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
See full target information MBP
style
left-column

文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Molecular brain 16:59 PubMed37438826

2023

Ameliorative effects of Fingolimod (FTY720) on microglial activation and psychosis-related behavior in short term cuprizone exposed mice.

Applications

Unspecified application

Species

Unspecified reactive species

Siyao Li,Koki Sakurai,Masahiro Ohgidani,Takahiro A Kato,Takatoshi Hikida

BMC biology 21:113 PubMed37221592

2023

CLARITY increases sensitivity and specificity of fluorescence immunostaining in long-term archived human brain tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Woelfle,Dhruva Deshpande,Simone Feldengut,Heiko Braak,Kelly Del Tredici,Francesco Roselli,Karl Deisseroth,Jens Michaelis,Tobias M Boeckers,Michael Schön

Bioactive materials 6:638-654 PubMed33005828

2020

Application of bioactive hydrogels combined with dental pulp stem cells for the repair of large gap peripheral nerve injuries.

Applications

Unspecified application

Species

Unspecified reactive species

Lihua Luo,Yan He,Ling Jin,Yanni Zhang,Fernando P Guastaldi,Abdullkhaleg A Albashari,Fengting Hu,Xiaoyan Wang,Lei Wang,Jian Xiao,Lingli Li,Jianming Wang,Akon Higuchi,Qingsong Ye

Scientific reports 9:8921 PubMed31222141

2019

Engineering a 3D functional human peripheral nerve in vitro using the Nerve-on-a-Chip platform.

Applications

Unspecified application

Species

Unspecified reactive species

Anup D Sharma,Laurie McCoy,Elizabeth Jacobs,Hannah Willey,Jordan Q Behn,Hieu Nguyen,Brad Bolon,J Lowry Curley,Michael J Moore

Biochimica et biophysica acta. Molecular and cell 1862:485-495 PubMed28185952

2017

Region-specific vulnerability to lipid peroxidation and evidence of neuronal mechanisms for polyunsaturated fatty acid biosynthesis in the healthy adult human central nervous system.

Applications

WB

Species

Human

Alba Naudí,Rosanna Cabré,Mayelin Dominguez-Gonzalez,Victoria Ayala,Mariona Jové,Natalia Mota-Martorell,Gerard Piñol-Ripoll,Maria Pilar Gil-Villar,Montserrat Rué,Manuel Portero-Otín,Isidre Ferrer,Reinald Pamplona
View all publications
style
left-column
style
left-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
style
left-column
style
right-column

关联产品

Select an associated product type
Alternative Product
Primary Antibodies

AB124493

Anti-Myelin Basic Protein antibody

primary-antibodies

myelin-basic-protein-antibody-ab124493

0

(0 reviews)

Alternative Product
Primary Antibodies

AB77895

HRP Anti-Myelin Basic Protein antibody

primary-antibodies

hrp-myelin-basic-protein-antibody-ab77895

5

(2 reviews)

Alternative Product
Primary Antibodies

AB65988

Anti-Myelin Basic Protein antibody

primary-antibodies

myelin-basic-protein-antibody-ab65988

0

(1 reviews)

Alternative Product
Primary Antibodies

AB11223

Anti-Myelin Basic Protein antibody [22]

primary-antibodies

myelin-basic-protein-antibody-22-ab11223

0

(0 reviews)

Alternative Product
Primary Antibodies

AB134018

Anti-Myelin Basic Protein antibody

primary-antibodies

myelin-basic-protein-antibody-ab134018

5

(3 reviews)

Alternative Product
Primary Antibodies

AB62631

Anti-Myelin Basic Protein antibody [MBP101] - BSA and Azide free

primary-antibodies

myelin-basic-protein-antibody-mbp101-bsa-and-azide-free-ab62631

4

(16 reviews)

Alternative Product
Primary Antibodies

AB216590

Anti-Myelin Basic Protein antibody

primary-antibodies

myelin-basic-protein-antibody-ab216590

0

(0 reviews)

Alternative Product
Primary Antibodies

AB40390

Anti-Myelin Basic Protein antibody - Oligodendrocyte Marker

primary-antibodies

myelin-basic-protein-antibody-oligodendrocyte-marker-ab40390

5

(20 reviews)

Alternative Product
Primary Antibodies

AB216668

Anti-Myelin Basic Protein antibody [IGX3421R-1] - Oligodendrocyte Marker

primary-antibodies

myelin-basic-protein-antibody-igx3421r-1-oligodendrocyte-marker-ab216668

5

(6 reviews)

Alternative Product
Primary Antibodies

AB209328

Anti-Myelin Basic Protein antibody [IGX3421] - Oligodendrocyte Marker

primary-antibodies

myelin-basic-protein-antibody-igx3421-oligodendrocyte-marker-ab209328

5

(10 reviews)

Alternative Product
Primary Antibodies

AB7349

Anti-Myelin Basic Protein antibody [12] - Oligodendrocyte Marker

primary-antibodies

myelin-basic-protein-antibody-12-oligodendrocyte-marker-ab7349

4

(22 reviews)

Alternative Product
Primary Antibodies

AB218011

Anti-Myelin Basic Protein antibody [EPR21188] - Oligodendrocyte Marker

primary-antibodies

myelin-basic-protein-antibody-epr21188-oligodendrocyte-marker-ab218011

5

(4 reviews)

Alternative Product
Primary Antibodies

AB277483

Anti-Myelin Basic Protein antibody [12] - BSA and Azide free

primary-antibodies

myelin-basic-protein-antibody-12-bsa-and-azide-free-ab277483

0

(0 reviews)

Alternative Product
Primary Antibodies

AB230378

Anti-Myelin Basic Protein antibody [EPR21188] - BSA and Azide free

primary-antibodies

myelin-basic-protein-antibody-epr21188-bsa-and-azide-free-ab230378

4

(1 reviews)

Alternative Product
Primary Antibodies

AB28541

Anti-Myelin Basic Protein antibody

primary-antibodies

myelin-basic-protein-antibody-ab28541

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com