Anti-Myelin Basic蛋白抗体(ab134018)
Key features and details
- Chicken polyclonal to Myelin Basic Protein
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgY
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-Myelin Basic蛋白抗体
参阅全部 Myelin Basic Protein 一抗 -
描述
鸡多克隆抗体to Myelin Basic蛋白 -
宿主
Chicken -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Mouse -
免疫原
A synthetic peptide, conjugated to KLH, corresponding to a region with the sequence of Human (NP_002376) and Mouse (NP_034907) Myelin Basic Protein.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
存储溶液
pH: 7
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
Immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 µg/ml, and the preparation was filter-sterilized. -
克隆
多克隆 -
同种型
IgY -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134018于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
---|
IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. -
组织特异性
MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. -
疾病相关
Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease). -
序列相似性
Belongs to the myelin basic protein family. -
发展阶段
Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter. -
翻译后修饰
Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated. -
细胞定位
Myelin membrane. Cytoplasmic side of myelin. - Information by UniProt
-
数据库链接
- Entrez Gene: 17196 Mouse
- SwissProt: P04370 Mouse
- Unigene: 252063 Mouse
- Unigene: 392350 Mouse
- Unigene: 454459 Mouse
-
别名
- GDB antibody
- Golli MBP antibody
- Golli MBP; myelin basic protein antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody (ab134018)Immunohistochemical analysis of a section of adult mouse brain labelling Myelin Basic Protein (red staining) of white matter tracts adjacent to the hippocampal formation with ab134018 at 1/1000 dilution. Green staining is autofluorescence from green fluorescent protein (GFP) expressed in this transgenic mouse.
实验方案
数据表及文件
-
Datasheet download
文献 (7)
ab134018 被引用在 7 文献中.
- Fountain C et al. Acquisition and Analysis of Excised Neocortex from Pediatric Patients with Focal Cortical Dysplasia Using Mesoscale Diffusion MRI. Diagnostics (Basel) 13:N/A (2023). PubMed: 37174921
- Kapitza C et al. Murine Esophagus Expresses Glial-Derived Central Nervous System Antigens. Int J Mol Sci 22:N/A (2021). PubMed: 33810144
- Rivera RR et al. Conditional Lpar1 gene targeting identifies cell types mediating neuropathic pain. FASEB J 34:8833-8842 (2020). PubMed: 32929779
- Gorgun MF et al. Elevated Neuroglobin Lessens Neuroinflammation and Alleviates Neurobehavioral Deficits Induced by Acute Inhalation of Combustion Smoke in the Mouse. Neurochem Res 44:2170-2181 (2019). PubMed: 31420834
- Kanda H et al. TREK-1 and TRAAK Are Principal K+ Channels at the Nodes of Ranvier for Rapid Action Potential Conduction on Mammalian Myelinated Afferent Nerves. Neuron 104:960-971.e7 (2019). PubMed: 31630908
- Beyer BA et al. Metabolomics-based discovery of a metabolite that enhances oligodendrocyte maturation. Nat Chem Biol 14:22-28 (2018). ICC/IF ; Mouse . PubMed: 29131145
- Modo M et al. Detection of aberrant hippocampal mossy fiber connections: Ex vivo mesoscale diffusion MRI and microtractography with histological validation in a patient with uncontrolled temporal lobe epilepsy. Hum Brain Mapp 37:780-95 (2016). PubMed: 26611565