重组Anti-Myelin Basic蛋白抗体[12] (ab7349)
![Immunocytochemistry/ Immunofluorescence - Anti-Myelin Basic Protein antibody [12] (ab7349)](https://www.abcam.cn/ps/products/7/ab7349/Images/ab7349-535819-anti-myelin-basic-protein-antibody-12-mouse-primary-neural-glia-cell.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Myelin Basic Protein antibody [12] (ab7349)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [12] to Myelin Basic Protein
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-Myelin Basic蛋白抗体[12]
参阅全部 Myelin Basic Protein 一抗 -
描述
大鼠单克隆抗体[12] to Myelin Basic蛋白 -
宿主
Rat -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Full length protein corresponding to Cow Myelin Basic Protein.
-
表位
Amino acids 82-87 (DENPVV). -
阳性对照
- WB: Human cerebellum, Mouse brain tissue and Rat brain tissue. IHC-P: Human cerebellum tissue. ICC/IF: Mouse primary oligodendroglia cells.
-
常规说明
This product has switched from a hybridoma to recombinant production method on 08th November 2021.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Ion Exchange Chromatography -
克隆
单克隆 -
克隆编号
12 -
同种型
IgG2a -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/200.
|
|
| WB | (2) |
1/1000.
|
| IHC-P | (7) |
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
ICC/IF
1/200. |
|
WB
1/1000. |
|
IHC-P
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. -
组织特异性
MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. -
疾病相关
Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease). -
序列相似性
Belongs to the myelin basic protein family. -
发展阶段
Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter. -
翻译后修饰
Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated. -
细胞定位
Myelin membrane. Cytoplasmic side of myelin. - Information by UniProt
-
数据库链接
- Entrez Gene: 4155 Human
- Entrez Gene: 17196 Mouse
- Entrez Gene: 24547 Rat
- Omim: 159430 Human
- SwissProt: P02686 Human
- SwissProt: P04370 Mouse
- SwissProt: P02688 Rat
- Unigene: 551713 Human
see all -
别名
- GDB antibody
- Golli MBP antibody
- Golli MBP; myelin basic protein antibody
see all
图片
-
Immunocytochemistry/ Immunofluorescence - Anti-Myelin Basic Protein antibody [12] (ab7349)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labelling Myelin Basic Protein with ab7349 at a 1/200 dilution, followed by Goat anti-Rat secondary (Alexa Fluor® 488) (ab150157) secondary antibody at a 1/1000 dilution. Confocal image showing positive staining in mouse primary oligodendroglia cells (shown in green). Nuclear DNA was labeled with DAPI (shown in blue).
Counterstained with Anti-MAP2 antibody (ab183830) at a 1/1000 dilution, followed by Goat Anti-Rabbit secondary (Alexa Fluor® 594) (ab150080) at a 1/1000 dilution (shown in magenta).
Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
The negative controls are as follows:
-ve control 1: Myelin Basic Protein (ab7349) at a 1/200 dilution, followed by Goat anti-Rabbit secondary (Alexa Fluor® 594) (ab150080) at a 1/1000 dilution.
-ve control 2: Anti-MAP2 (ab183830) at a 1/1000 dilution, followed by Goat anti-Rat secondary (Alexa Fluor® 488) (ab150157) at a 1/1000 dilution. -
![Western blot - Anti-Myelin Basic Protein antibody [12] (ab7349)](https://www.abcam.cn/ps/products/7/ab7349/Images/ab7349-480715-anti-cd105-antibody-mj7-18-western-blot-human-cerebellum.jpg)
Western blot - Anti-Myelin Basic Protein antibody [12] (ab7349)Anti-Myelin Basic Protein antibody [12] (ab7349) at 1/5000 dilution + Human cerebellum tissue lysate at 10 µg
Secondary
Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution
Developed using the ECL technique.
Observed band size: 18.5 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondThe molecular weight observed was in consistency with the literature (PMID: 9299539).
Blocking buffer: 5% NFDM/TBST.
-
![Western blot - Anti-Myelin Basic Protein antibody [12] (ab7349)](https://www.abcam.cn/ps/products/7/ab7349/Images/ab7349-480716-anti-cd105-antibody-mj7-18-western-blot-mouse-rat-brain.jpg)
Western blot - Anti-Myelin Basic Protein antibody [12] (ab7349)All lanes : Anti-Myelin Basic Protein antibody [12] (ab7349) at 1/5000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution
Observed band size: 14-21.5 kDa why is the actual band size different from the predicted?The molecular weight observed was in consistency with the literature (PMID: 9299539).
Blocking buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 3 seconds; Lane 2: 1 second.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] (ab7349)](https://www.abcam.cn/ps/products/7/ab7349/Images/ab7349-480717-anti-cd105-antibody-mj7-18-immunohistochemistry-human-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] (ab7349)Immunohistochemical analysis of paraffin-embedded Human cerebrum labeling Myelin Basic Protein with ab7349 at 1/4000 dilution (0.259 µg/ml) followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer; ab214882). The section was incubated with ab7349 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] (ab7349)](https://www.abcam.cn/ps/products/7/ab7349/Images/ab7349-480718-anti-cd105-antibody-mj7-18-immunohistochemistry-human-cerebrum-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myelin Basic Protein antibody [12] (ab7349)Immunohistochemical analysis of paraffin-embedded Human cerebrum labeling Myelin Basic Protein with ab7349 at 1/4000 dilution (0.259 µg/ml) followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer; ab214882). The section was incubated with ab7349 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with DAPI.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (309)
ab7349 被引用在 309 文献中.
- Eftekhari R et al. Blockade of Proteinase-Activated Receptor 2 (PAR2) Attenuates Neuroinflammation in Experimental Autoimmune Encephalomyelitis. J Pharmacol Exp Ther 388:12-22 (2024). PubMed: 37699708
- Wu L et al. RIT1 regulation of CNS lipids RIT1 deficiency Alters cerebral lipid metabolism and reduces white matter tract oligodendrocytes and conduction velocities. Heliyon 9:e20384 (2023). PubMed: 37780758
- Smith BC et al. Astrocyte interferon-gamma signaling dampens inflammation during chronic central nervous system autoimmunity via PD-L1. J Neuroinflammation 20:234 (2023). PubMed: 37828609
- Mustapha O et al. Neocortex neurogenesis and maturation in the African greater cane rat. Neural Dev 18:7 (2023). PubMed: 37833718
- Suhail H et al. An early glycolysis burst in microglia regulates mitochondrial dysfunction in oligodendrocytes under neuroinflammation. iScience 26:107921 (2023). PubMed: 37841597