Anti-MyD88抗体

• WB

Lab

Western blot - Anti-MyD88 antibody (AB2064)

Western blot : Anti-MYD88 antibody (ab2064) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab2064 was shown to bind specifically to MYD88. A band was observed at 35 kDa in wild-type A549 cell lysates with no signal observed at this size in MYD88 knockout cell line ab286715 (knockout cell lysate ab290793). To generate this image, wild-type and MYD88 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-MyD88 antibody (ab2064) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

MYD88 knockout A549 cell lysate at 20 µg

Lane 3:

HEK-293 cell lysate at 20 µg

Observed band size: 35 kDa

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• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody (AB2064)

Immunocytochemistry/ Immunofluorescence analysis of 4% paraformaldehyde fixed K562 cells labeling MyD88 with ab2064 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody (AB2064)

Immunocytochemistry/ Immunofluorescence analysis of 4% paraformaldehyde fixed Jurkat cells labeling MyD88 with ab2064 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-MyD88 antibody (AB2064)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling MyD88 with ab2064 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyD88 antibody (AB2064)

Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-MYD88 antibody (ab2064) at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyD88 antibody (AB2064)

Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-MYD88 antibody (ab2064) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• WB

Supplier Data

Western blot - Anti-MyD88 antibody (AB2064)

1 h incubation at RT in 5% NFDM/TBST.

beta-actin was used as a loading control at 1 µg/mL.

All lanes:

Western blot - Anti-MyD88 antibody (ab2064) at 2 µg/mL

Lane 1:

HeLa WT cell lysate at 10 µg

Lane 2:

MyD88 KO HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 33 kDa

false

• WB

Supplier Data

Western blot - Anti-MyD88 antibody (AB2064)

All lanes:

Western blot - Anti-MyD88 antibody (ab2064) at 2 µg/mL

Lane 1:

A549 cell lysate at 15 µg

Lane 2:

HepG2 cell lysate at 15 µg

Lane 3:

K562 cell lysate at 15 µg

Lane 4:

HT29 cell lysate at 15 µg

Lane 5:

HEK293 cell lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 33 kDa

false

• WB

Supplier Data

Western blot - Anti-MyD88 antibody (AB2064)

1 h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-MyD88 antibody (ab2064) at 2 µg/mL

Lane 1:

WT HeLa Cells at 10 µg

Lane 2:

MyD88 knockout HeLa Cells at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 33 kDa

false

• WB

AbReview47356****

Western blot - Anti-MyD88 antibody (AB2064)

All lanes:

Western blot - Anti-MyD88 antibody (ab2064) at 1 µg/mL

Lanes 1 - 2:

Wild type MEFs whole cell lysate

Lanes 3 - 4:

MyD88 knockout MEFs whole cell lysate

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa

true

Exposure time: 15s

This image is courtesy of an anonymous Abreview