Anti-MyD88抗体(ab131071)
Key features and details
- Rabbit polyclonal to MyD88
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-MyD88抗体
参阅全部 MyD88 一抗 -
描述
兔多克隆抗体to MyD88 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Rat, Human
预测可用于: Mouse -
免疫原
Synthetic peptide within Human MyD88 aa 150-250 (internal sequence). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Immunogen corresponds to a sequence in the middle region of Human MyD88 that is different from the related rat and mouse sequences by one amino acid.
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阳性对照
- WB: Rat spleen, thymus tissue lysate; Jurkat, Raji cell lysate. IHC-P: Human tonsil tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131071于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 33 kDa.
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml.
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说明 |
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WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 33 kDa. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. |
靶标
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功能
Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. -
组织特异性
Ubiquitous. -
疾病相关
Defects in MYD88 are the cause of MYD88 deficiency (MYD88D) [MIM:612260]; also known as recurrent pyogenic bacterial infections due to MYD88 deficiency. Patients suffer from autosomal recessive, life-threatening, often recurrent pyogenic bacterial infections, including invasive pneumococcal disease, and die between 1 and 11 months of age. Surviving patients are otherwise healthy, with normal resistance to other microbes, and their clinical status improved with age. -
序列相似性
Contains 1 death domain.
Contains 1 TIR domain. -
结构域
The intermediate domain (ID) is required for the phosphorylation and activation of IRAK. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 4615 Human
- Entrez Gene: 17874 Mouse
- Entrez Gene: 301059 Rat
- Omim: 602170 Human
- SwissProt: Q99836 Human
- SwissProt: P22366 Mouse
- SwissProt: Q6Y1S1 Rat
- Unigene: 82116 Human
see all -
别名
- Mutant myeloid differentiation primary response 88 antibody
- MYD 88 antibody
- Myd88 antibody
see all
图片
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All lanes : Anti-MyD88 antibody (ab131071) at 0.5 µg/ml
Lane 1 : Rat spleen tissue lysate
Lane 2 : Rat thymus tissue lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Predicted band size: 33 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MyD88 antibody (ab131071)Paraffin embedded human tonsil tissue stained for MyD88 using ab131071 at 1 µg/ml in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab131071 被引用在 4 文献中.
- Liang Y et al. Downregulation of fibromodulin attenuates inflammatory signaling and atrial fibrosis in spontaneously hypertensive rats with atrial fibrillation via inhibiting TLR4/NLRP3 signaling pathway. Immun Inflamm Dis 11:e1003 (2023). PubMed: 37904680
- Xinshuang Z et al. Compound Gaoziban tablet alleviates depression toll-like receptor 4/myeloid differentiation factor 88/nuclear factor-kappa B pathway. J Tradit Chin Med 42:956-964 (2022). PubMed: 36378054
- Yan S et al. Protective effect of glycyrrhizic acid on cerebral ischemia/reperfusion injury via inhibiting HMGB1-mediated TLR4/NF-κB pathway. Biotechnol Appl Biochem 66:1024-1030 (2019). PubMed: 31545873
- Sun L et al. Inhibition of HMGB1 reduces rat spinal cord astrocytic swelling and AQP4 expression after oxygen-glucose deprivation and reoxygenation via TLR4 and NF-?B signaling in an IL-6-dependent manner. J Neuroinflammation 14:231 (2017). PubMed: 29178911