Anti-Mycobacterium tuberculosis Ag85B抗体(ab43019)
Key features and details
- Rabbit polyclonal to Mycobacterium tuberculosis Ag85B
- Suitable for: IHC-P, WB, ELISA
- Isotype: IgG
概述
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产品名称
Anti-Mycobacterium tuberculosis Ag85B抗体
参阅全部 Mycobacterium tuberculosis Ag85B 一抗 -
描述
兔多克隆抗体to Mycobacterium tuberculosis Ag85B -
宿主
Rabbit -
特异性
This antibody is specific for the Ag85B protein in sera, plasma or cell culture supernatant. -
经测试应用
适用于: IHC-P, WB, ELISAmore details -
种属反应性
与反应: Mycobacterium tuberculosis -
免疫原
Recombinant full length protein (Mycobacterium tuberculosis)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 0.05% Glycerol (glycerin, glycerine), 0.6057% Tris, 1.2114% Sodium citrate -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab43019于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) |
Use at an assay dependent concentration.
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| WB | (2) |
1/3000 - 1/8000. Predicted molecular weight: 34 kDa.
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| ELISA | (1) |
1/5000 - 1/10000.
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| 说明 |
|---|
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IHC-P
Use at an assay dependent concentration. |
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WB
1/3000 - 1/8000. Predicted molecular weight: 34 kDa. |
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ELISA
1/5000 - 1/10000. |
靶标
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相关性
Antigen 85B is the most abundant protein expressed by Mycobacterium tuberculosis (about one quarter). It is a myocolyc transferase in the myc pathway and catalyses - like Ag85A and Ag85C - the transfer of the fatty acid mycolate from one trehalose monomycolate to another, resulting in trehalose dimycolate and free trehalose and helping build the cell wall. -
别名
- 30 kDa extracellular protein antibody
- Antigen 85 complex B antibody
- Mycobacterium antibody
- Mycolyl transferase 85B antibody
图片
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Western blot - Anti-Mycobacterium tuberculosis Ag85B antibody (ab43019)This image is courtesy of an anonymous AbreviewAll lanes : Anti-Mycobacterium tuberculosis Ag85B antibody (ab43019) at 1/5000 dilution
All lanes : E coli, expressing his-tagged Ag85b
Secondary
Lane 1 : HRP conjugated goat anti-rabbit IgG
Lane 2 : HRP conjugated goat anti-rat IgG
Predicted band size: 34 kDa
Observed band size: 34 kDa
Additional bands at: 28 kDa (possible non-specific binding), 28 kDa (possible degradation product)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mycobacterium tuberculosis Ag85B antibody (ab43019)This image is courtesy of an anonymous Abreviewab17976 staining Mycobacterium tuberculosis Ag85B in Human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde. Samples were incubated with primary antibody (1/100 in antibody diluent) for 1 hour at 25°C. A HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab43019 被引用在 18 文献中.
- Zhou Y et al. Aptamer Detection of Mycobaterium tuberculosis Mannose-Capped Lipoarabinomannan in Lesion Tissues for Tuberculosis Diagnosis. Front Cell Infect Microbiol 11:634915 (2021). PubMed: 33791241
- Wang J et al. Mycobacterium tuberculosis protein kinase G acts as an unusual ubiquitinating enzyme to impair host immunity. EMBO Rep 22:e52175 (2021). PubMed: 33938130
- Carranza C et al. A Dual Marker for Monitoring MDR-TB Treatment: Host-Derived miRNAs and M. tuberculosis-Derived RNA Sequences in Serum. Front Immunol 12:760468 (2021). PubMed: 34804048
- Huaman MA et al. Mycobacterium bovis Bacille-Calmette-Guérin Infection Aggravates Atherosclerosis. Front Immunol 11:607957 (2020). PubMed: 33391278
- Jain N et al. Mesenchymal stem cells offer a drug-tolerant and immune-privileged niche to Mycobacterium tuberculosis. Nat Commun 11:3062 (2020). PubMed: 32546788