Anti-Myc tag抗体- ChIP Grade (ab9132)
Key features and details
- Goat polyclonal to Myc tag
- Suitable for: IP, ChIP, WB, ICC, ELISA
- Isotype: IgG
概述
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产品名称
Anti-Myc tag抗体- ChIP Grade
参阅全部 Myc tag 一抗 -
描述
山羊多克隆抗体to Myc tag -
宿主
Goat -
特异性
Antibody was verified by ELISA against peptide conjugated to BSA (EQKLISEEDL /BSA). -
经测试应用
适用于: IP, ChIP, WB, ICC, ELISAmore details -
免疫原
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.80
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
应用
应用 | Ab评论 | 说明 |
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IP |
Use at 1-4 µg/mg of lysate.
Also PubMed: 20224777
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ChIP | (2) |
Use at an assay dependent concentration. ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads.
|
WB | (3) |
1/1000 - 1/30000.
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ICC |
1/200 - 1/2000.
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ELISA |
1/100 - 1/500.
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说明 |
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IP
Use at 1-4 µg/mg of lysate. Also PubMed: 20224777
|
ChIP
Use at an assay dependent concentration. ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. |
WB
1/1000 - 1/30000. |
ICC
1/200 - 1/2000. |
ELISA
1/100 - 1/500. |
靶标
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相关性
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
细胞定位
Nuclear -
别名
- c-myc tag antibody
- Myc Epitope Tag antibody
图片
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All lanes : Anti-Myc tag antibody - ChIP Grade (ab9132) at 0.04 µg/ml
Lane 1 : E. coli whole cell lysate at 0.2 µg
Lane 2 : E. coli whole cell lysate at 0.1 µg
Lane 3 : E. coli whole cell lysate at 0.05 µg -
A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a Myc or His - tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (151)
ab9132 被引用在 151 文献中.
- Higerd-Rusli GP et al. The fates of internalized NaV1.7 channels in sensory neurons: Retrograde cotransport with other ion channels, axon-specific recycling, and degradation. J Biol Chem 299:102816 (2023). PubMed: 36539035
- Ballmer D et al. HP1 proteins regulate nucleolar structure and function by secluding pericentromeric constitutive heterochromatin. Nucleic Acids Res 51:117-143 (2023). PubMed: 36533441
- Palicharla VR et al. Interactions between TULP3 tubby domain and ARL13B amphipathic helix promote lipidated protein transport to cilia. Mol Biol Cell 34:ar18 (2023). PubMed: 36652335
- Dou C et al. Sialylation of TLR2 initiates osteoclast fusion. Bone Res 10:24 (2022). PubMed: 35232979
- Ermakova M et al. Enhanced abundance and activity of the chloroplast ATP synthase in rice through the overexpression of the AtpD subunit. J Exp Bot 73:6891-6901 (2022). PubMed: 35904136