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AB9132

Anti-Myc tag 抗体 - ChIP Grade

Anti-Myc tag antibody - ChIP Grade

4

(7 Reviews)

|

(171 Publications)

Goat Polyclonal Myc tag antibody. Suitable for ICC, IP, ChIP, ELISA, WB and reacts with Tag samples. Cited in 171 publications.

查看别名

Myc Epitope Tag, c-myc tag

2 Images
ChIP - Anti-Myc tag antibody - ChIP Grade (AB9132)
  • ChIP

Unknown

ChIP - Anti-Myc tag antibody - ChIP Grade (AB9132)

A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a Myc or His - tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).

Western blot - Anti-Myc tag antibody - ChIP Grade (AB9132)
  • WB

Supplier Data

Western blot - Anti-Myc tag antibody - ChIP Grade (AB9132)

All lanes:

Western blot - Anti-Myc tag antibody - ChIP Grade (ab9132) at 0.04 µg/mL

Lane 1:

E. coli whole cell lysate at 0.2 µg

Lane 2:

E. coli whole cell lysate at 0.1 µg

Lane 3:

E. coli whole cell lysate at 0.05 µg

Predicted band size: 49 kDa

false

关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

应用

ELISA, IP, WB, ChIP, ICC

applications

特异性

Antibody was verified by ELISA against peptide conjugated to BSA (EQKLISEEDL /BSA).

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1-4 µg/mg of lysate", "IP-species-notes": "<p>Also PubMed: 20224777</p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "<p>ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads.</p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/3000", "WB-species-notes": "<p></p>" } } }

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
Antibodies were affinity purified using the peptide immobilized on solid support.Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
存储溶液
pH: 6.8 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

文献 (171)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:422 PubMed40998798

2025

TRIM21 promotes colorectal cancer development through regulating DNA replication by TCF3/MCM2/5 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xintian Zhang,Han Yao,Yichao Hou,Kun Zhou,Yu Liang,Lidan Hou,Xingming Zhang,Wenfeng Wang,Leilei Du,Mengfei Yao,Jianhua Wang,Xiangjun Meng

Oncology letters 30:446 PubMed40740985

2025

circEFR3A promotes breast cancer progression by sponging miR-590-3p to upregulate androgen receptor expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yunzhe Mi,Xinle Wang,Han Song,Zhenyu Wu,Sainan Li,Fei Liu,Wei Liu,Meixiang Sang,Cuizhi Geng

Development (Cambridge, England) 152: PubMed40326503

2025

The Rho GEF Trio functions in contact inhibition of locomotion of neural crest cells by interacting with Ptk7.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Till,Annette Borchers

Nature 637:496-503 PubMed39633052

2024

Mis-splicing of a neuronal microexon promotes CPEB4 aggregation in ASD.

Applications

Unspecified application

Species

Unspecified reactive species

Carla Garcia-Cabau,Anna Bartomeu,Giulio Tesei,Kai Chit Cheung,Julia Pose-Utrilla,Sara Picó,Andreea Balaceanu,Berta Duran-Arqué,Marcos Fernández-Alfara,Judit Martín,Cesare De Pace,Lorena Ruiz-Pérez,Jesús García,Giuseppe Battaglia,José J Lucas,Rubén Hervás,Kresten Lindorff-Larsen,Raúl Méndez,Xavier Salvatella

Signal transduction and targeted therapy 9:337 PubMed39617783

2024

Phosphorylation determines the glucose metabolism reprogramming and tumor-promoting activity of sine oculis homeobox 1.

Applications

Unspecified application

Species

Unspecified reactive species

Yanni Lin,Ling Li,Bin Yuan,Fei Luo,Xiujuan Zhang,Yuanjun Yang,Shaliu Luo,Jing Lin,Tianxing Ye,Youzhi Zhang,Shan Gao,Qinong Ye

Molecular biology of the cell 36:ar1 PubMed39565681

2024

A defined tubby domain β-barrel surface region of TULP3 mediates ciliary trafficking of diverse cargoes.

Applications

Unspecified application

Species

Unspecified reactive species

Vivek Reddy Palicharla,Hemant B Badgandi,Sun-Hee Hwang,Emilie Legué,Karel F Liem,Saikat Mukhopadhyay

The Plant journal : for cell and molecular biology 119:1558-1569 PubMed38865085

2024

Integration of C3H15-mediated transcriptional and post-transcriptional regulation confers plant thermotolerance in Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Guohua Chai,Huanhuan Liu,Yang Zhang,Congpeng Wang,Hua Xu,Guo He,Jie Meng,Xianfeng Tang,Dian Wang,Gongke Zhou

iScience 27:109768 PubMed38711441

2024

Molecular clues unveiling spinocerebellar ataxia type-12 pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Manish Kumar,Shweta Sahni,Vivekanand A,Deepak Kumar,Neetu Kushwah,Divya Goel,Himanshi Kapoor,Achal K Srivastava,Mohammed Faruq

eLife 13: PubMed38289024

2024

Heat stress-induced activation of MAPK pathway attenuates Atf1-dependent epigenetic inheritance of heterochromatin in fission yeast.

Applications

Unspecified application

Species

Unspecified reactive species

Li Sun,Libo Liu,Chunlin Song,Yamei Wang,Quan-Wen Jin

Cells 13: PubMed38247840

2024

Is Involved in Congenital Chylothorax.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan B Whitchurch,Sophia Schneider,Alina C Hilger,Ricarda Köllges,Jil D Stegmann,Lea Waffenschmidt,Laura Dyer,Holger Thiele,Bhanupriya Dhabhai,Tikam Chand Dakal,Andreas Müller,Dominic P Norris,Heiko M Reutter
View all publications

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