Anti-Myc tag 抗体

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus L2 protein with ab322522 at 1/100 (10.45 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing nuclear staining in 293T cells transfected with a Human papillomavirus type 16 L2 protein expression vector containing a myc-His-tag®(shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 (4μg/ml) dilution (Magenta).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus 6 L1 protein with ab322925 at 1/2000 dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing nuclear staining in 293T cells transfected with a human papillomavirus type 6B L1, (shown in green) but no staining in 293T cells transfected with a human papillomavirus type 11 L1 expression vector containing a myc tag. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human respiratory syncytial virus Fusion (F) Glycoprotein with ab322234 at 1/2000 (0.48 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells transfected with a Human respiratory syncytial virus subtype B1 Fusion (F) Glycoprotein expression vector containing a myc-His-tag® and weak staining in 293T cells transfected with a Human respiratory syncytial virus subtype A2 Fusion (F) Glycoprotein expression vector containing a myc-His-tag®(shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 2.5 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human respiratory syncytial virus Fusion (F) Glycoprotein with ab322869 at 1/100 dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a Human respiratory syncytial virus (RSV) (strain B1) Fusion glycoprotein expression vector containing a myc-His-tag® and weak staining in 293T cells transfected with a Human respiratory syncytial virus (RSV) (strain A2) Fusion glycoprotein expression vector containing a myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling BirA/TurboID/miniTurbo with ab324658 at 1/500 (2.2 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing positive staining in 293T cells transfected with an E. coli birA and E. coli TurboID and E. coli miniTurbo expression vector containing a myc-His-tag®(shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at (1/1000) 2 ug/ml dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at (1/1000) 2 ug/ml dilution (Magenta).

Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at (1/1000) 2 ug/ml dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells transfected with a HIV1 Rev M, HIV Rev N, a HIV1 Rev O and HIV2 Rev expression vector labelling HIV Rev with ab85529 at 1/500 dilution. ab85529 was incubated overnight at 4° C, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed (ab150117) at 1/1000 dilution at RT for 45 min. ab9106 Anti-Myc Rabbit polyclonal antibody at 1/200 dilution, was co-incubated with ab85529 overnight at 4° C. Nucleus were visualized using DAPI.

Confocal image showing positive staining in 293T cells transfected with a HIV1 Rev M and HIV Rev N expression vector containing a myc-His-tag®(shown in green). but no staining in 293T cells transfected with a HIV1 Rev O and HIV2 Rev expression vector containing a myc-His-tag®.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus 6 L1 protein with ab322925 at 1/2000 dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing nuclear staining in 293T cells transfected with a human papillomavirus type 6B L1 expression vector containing a myc tag (shown in green) but no staining in 293T cells transfected with a human papillomavirus type 18 L1 or human papillomavirus type 4 L1 expression vector containing a myc tag. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling HPV4 L2 with ab322958 at 1/100 dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing nuclear staining in 293T cells transfected with a Human papillomavirus type 4 L2 and Human papillomavirus type 65 L2 expression vector containing a myc-His-tag®(shown in green) but no staining in 293T cells transfected with a human papillomavirus type 60 L2 expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human respiratory syncytial virus phosphoprotein VP32 with ab322893 at 1/200 dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining both in 293T cells transfected with a human respiratory syncytial virus A (strain A2) Phosphoprotein VP32 and a human respiratory syncytial virus B (strain B1) Phosphoprotein VP32 expression vector containing a myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/1000 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized transfected 293T (human embryonic kidney epithelial cell) cells labelling HIV Vif with ab66643 at 1/100 dilution followed by secondary antibody ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluo^® 488) at 1/1000 dilution (green).

ab9106 Anti-Myc Rabbit polyclonal antibody was used as a counterstain at a 1/200 dilution followed by a secondary antibody ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) preadsorbed at a 1/1000 dilution (Magenta).

Confocal image showing positive staining in 293T cells transfected with human immunodeficiency virus type 1 group M subtype B (isolate YU-2) (HIV-1) Vif expression vector containing a myc-His-tag^® (shown in green) but no staining in 293T cells transfected with a human immunodeficiency virus type 1 group M subtype K (isolate 96CM-MP535) (HIV-1) Vif, human immunodeficiency virus type 1 group M subtype K (isolate 97ZR-EQTB11) (HIV-1) Vif, human immunodeficiency virus type 1 group M subtype D (isolate ELI) (HIV-1) Vif expression vector containing a myc-His-tag^®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
A : 293T cells transfected with an empty vector containing a myc-His-tag^®
B : 293T cells transfected with a human immunodeficiency virus type 1 group M subtype K (isolate 96CM-MP535) (HIV-1) Vif expression vector containing a myc-His-tag^®
C : 293T cells transfected with a human immunodeficiency virus type 1 group M subtype K (isolate 97ZR-EQTB11) (HIV-1) Vif expression vector containing a myc-His-tag^®
D : 293T cells transfected with a human immunodeficiency virus type 1 group M subtype B (isolate YU-2) (HIV-1) Vif expression vector containing a myc-His-tag^®
E : 293T cells transfected with a human immunodeficiency virus type 1 group M subtype D (isolate ELI) (HIV-1) Vif expression vector containing a myc-His-tag^®

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus type 11 L1 with ab317837 at 1/500 (2.062 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a HPV 11 L1 expression vector containing a myc-His-tag® (shown in green) but no staining in 293T cells transfected with a HPV 6B L1 or HPV13 L1 expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
A : Myc transfected cells
B : HPV 11 L1-Myc transfected cells
C : HPV 6B L1-Myc transfected cells
D : HPV13 L1-Myc transfected cells
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/100 (5 ug/ml) dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 (2μg/ml) dilution (Magenta).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling influenza A H1N1 (A/Brisbane/59/2007) neuraminidase with ab318986 at 1/2000 (0.451 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

Confocal image showing cytoplasmic and membranous staining in 293T cells transfected with a N1, H1N1, A/Brisbane/59/2007 expression vector containing a myc-His-tag® (shown in green) but no staining in 293T cells transfected with a N9, H7N9, A/Anhui/1/2013 or N1, H1N1, A/California/07/2009 vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
A : Myc transfected cells
B : N1, H1N1, A/Brisbane/59/2007-Myc transfected cells
C : N1, H1N1, A/California/07/2009-Myc transfected cells
D : N9, H7N9, A/Anhui/1/2013-Myc transfected cells
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 (10ug/ml) dilution (Magenta). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2ug/ml) dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus L2 protein with ab322523 at 1/1000 (1.04 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a Human papillomavirus type 18 L2 protein, a Human papillomavirus type 16 L2 protein, a Human papillomavirus type 31 L2 protein, a Human papillomavirus type 45 L2 protein, a Human papillomavirus type 51 L2 protein, a Human papillomavirus type 6B L2 protein and a Human papillomavirus type 11 L2 protein expression vector containing a myc-His-tag®(shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 (2μg/ml) dilution (Magenta).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Hepatitis C Virus region NS5B with ab322521 at 1/2000 (0.47 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a Hepatitis C virus genotype 2a (isolate JFH-1) (HCV) region NS5B expression vector containing a myc-His-tag®(shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 (2μg/ml) dilution (Magenta).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus type 11 L1 with ab317837 at 1/500 (2.062 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a HPV 11 L1 expression vector containing a myc-His-tag® (shown in green) but no staining in 293T cells transfected with a HPV 16 L1 or HPV 18 L1 expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
A : Myc transfected cells
B : HPV 11 L1-Myc transfected cells
C : HPV 18 L1-Myc transfected cells
D : HPV 16 L1-Myc transfected cells
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 (2μg/ml) dilution (Magenta).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus L2 protein with ab321817 at 1/2000 (0.51 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing nuclear and cytoplasmic staining in 293T cells transfected with a Human papillomavirus type 16 L2 protein expression vector containing a myc-His-tag®(shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 (4 ug/ml) dilution (Magenta).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 100% Methanol permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Influenza A virus M1 protein with ab322959 at 1/2000 (0.50 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing nuclear staining in 293T cells transfected with a Influenza A virus M1 protein expression vector containing a myc tag (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

-ve control 1 : ab322959 at 1/2000 dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution.

-ve control 2 : ab9106 at 1/200 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human Papilloma Virus type 35 L1 protein with ab322518 at 1/100 (10.66 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

Confocal image showing nuclear staining in 293T cells transfected with a HPV 35 L1 expression vector containing a myc-His-tag®(shown in green) but no staining in 293T cells transfected with a HPV 16 L1 or HPV 11 L1 expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
A : Myc transfected cells
B : HPV 35 L1-Myc transfected cells
C : HPV 16 L1-Myc transfected cells
D : HPV 11 L1-Myc transfected cells
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 (2ug/ml) dilution (Magenta).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling influenza A H1N1 (A/California/07/2009) neuraminidase with ab318988 at 1/2000 (0.465 ug/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (2ug/ml) dilution (Green).

Confocal image showing cytoplasmic and membranous staining in 293T cells transfected with a N1, H1N1, A/California/07/2009 expression vector containing a myc-His-tag® (shown in green) but no staining in 293T cells transfected with a N9, H7N9, A/Anhui/1/2013 or N1, H1N1, A/Brisbane/59/2007 vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
A : Myc transfected cells
B : N1, H1N1, A/Brisbane/59/2007-Myc transfected cells
C : N1, H1N1, A/California/07/2009-Myc transfected cells
D : N9, H7N9, A/Anhui/1/2013-Myc transfected cells
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Magenta). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 (2ug/ml) dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus type 16 L1 protein with ab321901 at 1/2000 (0.57 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing nuclear staining in 293T cells transfected with a HPV 16 L1 protein expression vector containing a myc-His-tag®(shown in green) but no staining in 293T cells transfected with a HPV 18 L1 protein or HPV 6B L1 protein or HPV 11 L1 protein expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 2.5 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling MERS Spike glycoprotein with ab322146 at 1/500 dilution, followed by Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells transfected with a MERS-CoV S protein expression vector containing a myc-His-tag®(shown in green) and no staining in 293T cells transfected with a SARS-CoV-2 Spike glycoprotein expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor® 488) at 1/1000 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus type 59 L1 protein with ab322271 at 1/2000 (0.49ug/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a Human papillomavirus type 59 (HPV59) L1 protein expression vector containing a myc-His-tag®(shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 2.5 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 2ug/ml dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human respiratory syncytial virus Fusion (F) Glycoprotein with ab322235 at 1/2000 (0.56 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in 293T cells transfected with a Human respiratory syncytial virus subtype B1 Fusion (F) Glycoprotein expression vector containing a myc-His-tag® and weak staining in 293T cells transfected with a Human respiratory syncytial virus subtype A2 Fusion (F) Glycoprotein expression vector containing a myc-His-tag®(shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 2.5 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• IP

AbReview4357****

Immunoprecipitation - Anti-Myc tag antibody (AB9106)

ab9106 used at 1/250 in the immunoprecipitation of transfected human 293FT cells (whole cell lysate).

In this experiment cells were co-transfected with myc tagged cdc25 along with an HA tagged viral protein which binds to cdc25. The cell lysate was immunoprecipitated using ab9106, run on a 10% gel. The blot was probed for HA and HA tagged protein (which co-IPed with Myc tagged protein) and this is seen at ~15KDa.

Lane 1 : Input non-transfected cells

Lane 2 : Immunoprecipitated non-transfected cells

Lane 3 : Input co-transfected cells

Lane 4 : Immunoprecipitated co-transfected cells

This image is courtesy of an Abreview submitted on 23 March 2006.

All lanes:

Immunoprecipitation - Anti-Myc tag antibody (ab9106)

Predicted band size: 49 kDa

false

• IP

AbReview16699****

Immunoprecipitation - Anti-Myc tag antibody (AB9106)

ab9106 at 4 μg/mg lysate used in Huh7 (Human cell line) whole cell lysate (1x10⁶ cells).

Cells expressed myc-tagged CDC42 protein. Immunoprecipitation step performed using Protein A matrix. Incubation time 4 hours at 4°C. Western Blot antibody used at 1/300 dilution.

All lanes:

Immunoprecipitation - Anti-Myc tag antibody (ab9106)

Predicted band size: 49 kDa

false

This image is courtesy of an anonymous abreview.

• IHC-P

AbReview43124****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myc tag antibody (AB9106)

Paraformaldehyde-fixed, 0.3% Triton X-100 permeabilized mouse brain tissue stained for Myc tag using ab9106 at a 1/500 dilution (15 hrs, 4°C) followed by and Alexa-Fluor® 555 donkey anti-rabbit secondary used at a 1/500 dilution. For further details please see abreview.

This image is courtesy of Karen Lee by Abreview.

• IHC-Fr

AbReview13385****

Immunohistochemistry (Frozen sections) - Anti-Myc tag antibody (AB9106)

Paraformaldehyde-fixed frozen mouse brain tissue stained for Myc tag using ab9106 at a 1/500 dilution (2hrs at 25°C), followed by a goat anti-rabbit Alexa-Fluor^® 568 secondary used at a 1/500 dilution.

This image is courtesy of an anonymous abreview.

• EM

AbReview7103****

Electron Microscopy - Anti-Myc tag antibody (AB9106)

ab9106 at 1/2000 staining mouse embryonic fibroblasts expressing myc-tagged protein by ICC.

The cells were paraformaldehyde fixed and blocked with 3% BSA prior to incubation with the antibody for 1 hour. A goat anti-rabbit IgG antibody (10 nm gold conjugated) was used as the secondary.

The image shown is immuno electron microscopical staining with thawed cryosections.

This image is courtesy of an Abreview submitted by Dr Eeva-Liisa Eskelinen

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling HIV-1 gp140 with ab322236 at 1/100 (10 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a Human immunodeficiency virus type 1 group M subtype B (isolate HXB2) (HIV-1) Envelope glycoprotein gp160 expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney) transfected with myc-tagged Herpes simplex virus 1 gH expression vector cells labelling HSV1 gH with ab110227 at 1/200 (4.47 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic and membranous staining in 293T cells transfected with a Herpes simplex virus 1 gH expression vector containing a myc-His-tag.

Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Anti-Myc Rabbit polyclonal antibody (ab9106) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling SARS2-CoV2 Membrane Protein M with ab325776 at 1/500 dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a SARS-CoV2 membrane (M) protein and SARS-CoV membrane (M) protein expression vectors containing a myc-His-tag® (shown in green), showing no staining in 293T cells transfected with a MERS-Cov membrane (M) protein and HCoV-OC43 membrane (M) protein, HCoV-HKU1 membrane (M) protein expression vectors containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Panel A : 293T cells transfected with an empty expression vector containing a myc-His-tag®.
Panel B : 293T cells transfected with a SARS-CoV2 membrane (M) protein expression vector containing a myc-His-tag®.
Panel C : 293T cells transfected with a SARS-CoV membrane (M) protein expression vector containing a myc-His-tag®.
Panel D : 293T cells transfected with a MERS-Cov membrane (M) protein expression vector containing a myc-His-tag®.
Panel E : 293T cells transfected with a HCoV-OC43 membrane (M) protein expression vector containing a myc-His-tag®.
Panel F : 293T cells transfected with a HCoV-HKU1 membrane (M) protein expression vector containing a myc-His-tag®.

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/500 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% paraformaldehyde (PFA)-fixed, permeabilized with 0.1% Triton X-100 in CHO cells transfected with 12-tags constructs (named as CHO-12 tags, Top) and parental CHO cells (Bottom) labelling MYC with ab9106 at 1/1000 dilution (1 μg/mL), followed by Donkey Anti-rabbit IgG (H&L), FITC conjugated antibody at 1/1000 dilution (1 μg/mL) (Green). Nucleus was counterstained with DAPI (Blue).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling HIV1 p24 with ab85536 at 1/100 dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 dilution(Green).

Confocal image showing positive staining in 293T cells transfected with a Human immunodeficiency virus type 1 group M subtype B (isolate HXB2) (HIV-1) expression vector containing a myc-His-tag^® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counter stain Myc at a 1/200 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at a 1/500 dilution (Magenta).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Myc tag antibody (AB9106)

Confocal image showing positive staining in 293T cells transfected with an influenza A virus (H3N2) PB1 expression vector containing a myc-his-tag. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
A : 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-his-tag
B : 293T cells transfected with an influenza A virus (H3N2) PB1 expression vector containing a myc-his-tag
C : 293T cells transfected with an influenza B virus PB1 expression vector containing a myc-his-tag
D : 293T cells transfected with an influenza C virus PB1 expression vector containing a myc-his-tag

Primary antibody ab324448 used at a 1/100 (9.46 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/1000 (1 ug/ml) dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 (2 ug/ml) dilution (Magenta). Nuclear DNA was labelled with DAPI (shown in blue).

Cells were fixed in 4% Paraformaldehyde and permeabilized using 0.1% Triton X-100.

• WB

PubMed

Western blot - Anti-Myc tag antibody (AB9106)

Y. enterocolitica ΔHOPEMT carrying plasmids encoding Myc-tagged CT082 or CT695, and HA-tagged Slc1 (as indicated) were grown in non-secreting conditions. Chloramphenicol was added (time = 0 min) to stop bacterial protein synthesis and samples were taken at the indicated time points. Samples were analyzed by immunoblotting using ab9106, anti-HA, and anti-TEM antibodies.

All lanes:

Western blot - Anti-Myc tag antibody (ab9106)

Predicted band size: 49 kDa

false

Pais et al PLoS One. 2013;8(2):e56292. doi: 10.1371/journal.pone.0056292. Epub 2013 Feb 19. Fig 6. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/