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Anti-Myc tag抗体[9E10] - BSA and Azide free (ab264603)

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Western blot - Anti-Myc tag antibody [9E10] - BSA and Azide free (ab264603)
  • Western blot - Anti-Myc tag antibody [9E10] - ChIP Grade (ab264603)

Key features and details

  • Mouse monoclonal [9E10] to Myc tag - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt, WB, IP, ELISA, IHC-Fr, Purification
  • Reacts with: Species independent
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Myc tag抗体[9E10] - BSA and Azide free
    参阅全部 Myc tag 一抗

  • 描述

    小鼠单克隆抗体[9E10] to Myc tag - BSA and Azide free

  • 宿主

    Mouse

  • 经测试应用

    适用于: ICC/IF, Flow Cyt, WB, IP, ELISA, IHC-Fr, Purificationmore details

  • 种属反应性

    与反应: Species independent

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 表位

    aa 410-419 of human Myc.

  • 阳性对照

    • Myc tagged proteins and myc tag expressing cells.

  • 常规说明

    ab264603 is the carrier-free version of ab32.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • 存储溶液

    Constituent: PBS

  • 无载体

    是
  • Concentration information loading...

  • 纯度

    IgG fraction

  • 克隆

    单克隆

  • 克隆编号

    9E10

  • 骨髓瘤

    Sp2/0

  • 同种型

    IgG1

  • 轻链类型

    kappa

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab264603于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

See Abreviews.
Purification
Use at an assay dependent concentration.
说明
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

See Abreviews.
Purification
Use at an assay dependent concentration.

靶标

  • 相关性

    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.

  • 细胞定位

    Nuclear

  • 别名

    • c-myc tag antibody
    • Myc Epitope Tag antibody

图片

  • Western blot - Anti-Myc tag antibody [9E10] - BSA and Azide free (ab264603)
    Western blot - Anti-Myc tag antibody [9E10] - BSA and Azide free (ab264603)
    All lanes : Anti-Myc tag antibody [9E10] (ab32) at 1/200 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : MYC CRISPR-Cas9 edited HEK-293T cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : SH-SY5Y cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.


    False colour image of Western blot: Anti-Myc tag antibody [9E10] staining at 1/200 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab32 was shown to bind specifically to Myc tag. A band was observed at 57 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in MYC CRISPR-Cas9 edited cell line ab256500 (CRISPR-Cas9 edited cell lysate ab263850). The band observed in the CRISPR-Cas9 edited lysate lane below 57 kDa is likely to represent a truncated form of Myc tag. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and MYC CRISPR-Cas9 edited HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

  • Western blot - Anti-Myc tag antibody [9E10] - ChIP Grade (ab264603)
    Western blot - Anti-Myc tag antibody [9E10] - ChIP Grade (ab264603)
    Anti-Myc tag antibody [9E10] (ab32) at 1 µg/ml + E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 45 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, Azide and Arginine (ab32).

    Lysate from E. coli recombinantly expressing 11 commonly used tags including myc tag.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

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