重组Anti-MYBPC3抗体[EPR3009(2)] (ab133499)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3009(2)] to MYBPC3
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-MYBPC3抗体[EPR3009(2)]
参阅全部 MYBPC3 一抗 -
描述
兔单克隆抗体[EPR3009(2)] to MYBPC3 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human fetal heart, Rat heart, and Mouse heart lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3009(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133499于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 141 kDa).
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 141 kDa). |
靶标
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功能
Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. -
疾病相关
Defects in MYBPC3 are the cause of cardiomyopathy familial hypertrophic type 4 (CMH4) [MIM:115197]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. -
序列相似性
Belongs to the immunoglobulin superfamily. MyBP family.
Contains 3 fibronectin type-III domains.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains. -
翻译后修饰
Substrate for phosphorylation by PKA and PKC. Reversible phosphorylation appears to modulate contraction. - Information by UniProt
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数据库链接
- Entrez Gene: 4607 Human
- Entrez Gene: 17868 Mouse
- Entrez Gene: 295929 Rat
- Omim: 600958 Human
- SwissProt: Q14896 Human
- SwissProt: O70468 Mouse
- SwissProt: P56741 Rat
- Unigene: 524906 Human
see all -
别名
- C protein cardiac muscle isoform antibody
- C-protein antibody
- cardiac muscle isoform antibody
see all
图片
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All lanes : Anti-MYBPC3 antibody [EPR3009(2)] (ab133499) at 1/1000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Rat heart tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 141 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-MYBPC3 antibody [EPR3009(2)] (ab133499) at 1/1000 dilution
Lane 1 : Human heart lysate
Lane 2 : Rat heart lysate
Lane 3 : Mouse heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 141 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab133499 被引用在 2 文献中.
- Charman M et al. Constitutive TRIM22 Expression in the Respiratory Tract Confers a Pre-Existing Defence Against Influenza A Virus Infection. Front Cell Infect Microbiol 11:689707 (2021). PubMed: 34621686
- Da'as SI et al. Functional characterization of human myosin-binding protein C3 variants associated with hypertrophic cardiomyopathy reveals exon-specific cardiac phenotypes in zebrafish model. J Cell Physiol 235:7870-7888 (2020). PubMed: 31943169