重组Anti-MVK抗体[EPR6507] (ab126619)
![Western blot - Anti-MVK antibody [EPR6507] (ab126619)](https://www.abcam.cn/ps/products/126/ab126619/Images/ab126619-140611-anti-mvk-antibody-epr6507-western-blot.jpg "Western blot - Anti-MVK antibody [EPR6507] (ab126619)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6507] to MVK
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-MVK抗体[EPR6507]
参阅全部 MVK 一抗 -
描述
兔单克隆抗体[EPR6507] to MVK -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human MVK aa 300-400. The exact sequence is proprietary.
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阳性对照
- Permeabilized HepG2 cells; Human fetal liver, HepG2, Jurkat and Caco-2 cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 1.88 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6507 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126619于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). |
靶标
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功能
May be a regulatory site in cholesterol biosynthetic pathway. -
通路
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3. -
疾病相关
Defects in MVK are the cause of mevalonic aciduria (MEVA) [MIM:610377]. It is an accumulation of mevalonic acid which causes a variety of symptoms such as psychomotor retardation, dysmorphic features, cataracts, hepatosplenomegaly, lymphadenopathy, anemia, hypotonia, myopathy, and ataxia.
Defects in MVK are the cause of hyperimmunoglobulinemia D and periodic fever syndrome (HIDS) [MIM:260920]. HIDS is an autosomal recessive disease characterized by recurrent episodes of unexplained high fever associated with skin rash, diarrhea, adenopathy (swollen, tender lymph nodes), athralgias and/or arthritis. Concentration of IgD, and often IgA, are above normal. -
序列相似性
Belongs to the GHMP kinase family. Mevalonate kinase subfamily. -
细胞定位
Cytoplasm. Peroxisome. - Information by UniProt
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数据库链接
- Entrez Gene: 4598 Human
- Omim: 251170 Human
- SwissProt: Q03426 Human
- Unigene: 130607 Human
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别名
- FLJ96772 antibody
- KIME_HUMAN antibody
- LH receptor mRNA binding protein antibody
see all
图片
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Western blot - Anti-MVK antibody [EPR6507] (ab126619)All lanes : Anti-MVK antibody [EPR6507] (ab126619) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Caco-2 cell lysate
Lane 4 : Human fetal liver tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa -
![Flow Cytometry (Intracellular) - Anti-MVK antibody [EPR6507] (ab126619)](https://www.abcam.cn/ps/products/126/ab126619/Images/ab126619-140610-anti-mvk-antibody-epr6507-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-MVK antibody [EPR6507] (ab126619)ab126619, at a 1/10 dilution, positively staining MVK in permeabilized HepG2 cells (red). A Rabbit IgG was used as negative control (green).
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![OI-RD Scanning - Anti-MVK antibody [EPR6507] (ab126619)](https://www.abcam.cn/ps/products/126/ab126619/Images/ab126619-140609-anti-mvk-antibody-epr6507.jpg)
OI-RD Scanning - Anti-MVK antibody [EPR6507] (ab126619) -
![Anti-MVK antibody [EPR6507] (ab126619)](https://www.abcam.cn/ps/products/126/ab126619/Images/ab126619-4-benefits-of-recombinant-antibodies.png)
Anti-MVK antibody [EPR6507] (ab126619)
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab126619 被引用在 1 文献中.
- Buchou C et al. Upregulation of the Mevalonate Pathway through EWSR1-FLI1/EGR2 Regulatory Axis Confers Ewing Cells Exquisite Sensitivity to Statins. Cancers (Basel) 14:N/A (2022). PubMed: 35565457