AB109499

重组Anti-muscle Actin抗体[EPR4791]

Anti-muscle Actin antibody [EPR4791] - Loading Control

RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal alpha skeletal muscle Actin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

查看别名

ACTA, ACTA1, Alpha-actin-1

2 Images

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [EPR4791] - Loading Control (AB109499)

Immunohistochemical analysis of paraffin-embedded human colonic tissue using ab109499

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-muscle Actin antibody [EPR4791] - Loading Control (AB109499)

All lanes:

Western blot - Anti-muscle Actin antibody [EPR4791] - Loading Control (ab109499) at 1/1000 dilution

Lane 1:

Human skeletal muscle lysate at 10 µg

Lane 2:

Human heart lysates at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 42 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-muscle Actin antibody [EPR4791] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4791

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

The immunogen for this product has 100% homology with POTE ankyrin domain family member (121 kDa) and 100% homology with alpha cardiac muscle 1, aortic smooth muscle, alpha skeletal muscle and gamma-enteric smooth muscle (42 kDa). In our testing; this antibody only detected a 42 kDa band in Western blot.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Perform antigen retrieval", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Muscle Actin also known as ACTA1 is a protein that plays a significant role in muscle contraction. It has a molecular weight of approximately 42 kDa. This protein is primarily found in skeletal muscle tissues. Muscle Actin exists in two forms: G-actin a globular monomer and F-actin a filamentous polymer that forms actin filaments. These filaments provide structural support and are involved in muscle contraction through their interaction with myosin.

Biological function summary

Muscle Actin interacts with myosin to facilitate muscle contraction. It is an important component of the thin filament within the sarcomere the basic unit of muscle fiber. Muscle Actin is part of a complex and this complex includes other important proteins like tropomyosin and troponin. These proteins regulate the interaction between actin and myosin enabling the muscle contraction process. Muscle Actin's polymerization and depolymerization dynamics are important for the regulation of cellular movement and structural integrity.

Pathways

Muscle Actin is essential for the actin-myosin contractile machinery in muscle cells which is part of the sarcomere assembly pathway. This process involves other proteins such as myosin heavy chain and tropomyosin which are important for muscle contraction. Another important pathway is the Rho GTPase signaling pathway which controls actin filament assembly and is involved in a variety of cellular processes including muscle contraction and cell motility.

Muscle Actin mutations link to certain muscle-related diseases such as nemaline myopathy and congenital myopathies. Nemaline myopathy involves the presence of rod-like structures in muscle fibers and it results from anomalies in actin filaments. Muscle Actin also associates with cardiac muscle conditions when there are mutations affecting cardiac actin structure or function. This reveals the connection between Muscle Actin tropomyosin and troponin in muscle contraction efficiency and structural integrity contributing to these diseases' pathogenesis.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTA1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery 276:115-130 PubMed30446828

2018

Different effects of allergic rhinitis on nasal mucosa remodeling in chronic rhinosinusitis with and without nasal polyps.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Xiang,Qing-Ping Zhang,Wei Zhang,Yong-Gang Kong,Lu Tan,Shi-Ming Chen,Yu-Qin Deng,Ze-Zhang Tao,Yu Xu

View all publications

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Abcam Product Promise

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重组Anti-muscle Actin抗体[EPR4791]