Anti-Muscarinic Acetylcholine Receptor M5/CHRM5抗体(ab41171)
Key features and details
- Rabbit polyclonal to Muscarinic Acetylcholine Receptor M5/CHRM5
- Suitable for: ELISA, IHC-FoFr, IHC (PFA fixed), WB
- Reacts with: Rat
- Isotype: IgG
概述
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产品名称
Anti-Muscarinic Acetylcholine Receptor M5/CHRM5抗体
参阅全部 Muscarinic Acetylcholine Receptor M5/CHRM5 一抗 -
描述
兔多克隆抗体to Muscarinic Acetylcholine Receptor M5/CHRM5 -
宿主
Rabbit -
特异性
Cross reactivity: M5 Muscarinic Receptor (519-531): 100% M5 Muscarinic Receptor: 75% No cross reactivity was observed in: M1 Muscarinic Receptor(451-460) M1 Muscarinic Receptor M2 Muscarinic Receptor(457-466) M2 Muscarinic Receptor M3 Muscarinic Receptor(580-589) M3 Muscarinic Receptor M4 Muscarinic Receptor(469-478) M4 Muscarinic Receptor -
经测试应用
适用于: ELISA, IHC-FoFr, IHC (PFA fixed), WBmore details -
种属反应性
与反应: Rat -
免疫原
Synthetic peptide corresponding to Rat Muscarinic Acetylcholine Receptor M5/CHRM5 (C terminal).
Database link: P08911 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Reconstitute the lyophilized pooled antisera with 0.1 ml of 10 mg/ml BSA and 0.01% Sodium Azide in PBS for the equivalent of whole antisera, or with additional buffer for more dilute antisera. -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: Whole serum -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab41171于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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IHC-FoFr |
Use at an assay dependent concentration. PubMed: 23773170
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IHC (PFA fixed) |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 60 kDa.
Blocking with 5% goat or donkey serum significantly reduces background when compared with BSA or milk blocking. |
说明 |
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ELISA
Use at an assay dependent concentration. |
IHC-FoFr
Use at an assay dependent concentration. PubMed: 23773170 |
IHC (PFA fixed)
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 60 kDa. Blocking with 5% goat or donkey serum significantly reduces background when compared with BSA or milk blocking. |
靶标
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功能
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM5 sub-subfamily. -
细胞定位
Cell membrane. Cell junction, synapse, postsynaptic cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 53949 Rat
- SwissProt: P08911 Rat
- Unigene: 44293 Rat
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别名
- Acetylcholine receptor muscarinic 5 antibody
- AChR antibody
- AChR M5 antibody
see all
数据表及文件
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Datasheet download
文献 (6)
ab41171 被引用在 6 文献中.
- Underhill SM & Amara SG Acetylcholine Receptor Stimulation Activates Protein Kinase C Mediated Internalization of the Dopamine Transporter. Front Cell Neurosci 15:662216 (2021). PubMed: 33897375
- Zhu H et al. Impairments of spatial memory in an Alzheimer's disease model via degeneration of hippocampal cholinergic synapses. Nat Commun 8:1676 (2017). PubMed: 29162816
- Muise ED et al. Localization of muscarinic acetylcholine receptor 2 to the intestinal crypt stem cell compartment. Data Brief 10:482-486 (2017). PubMed: 28054012
- Muschter D et al. Reactivity of rat bone marrow-derived macrophages to neurotransmitter stimulation in the context of collagen II-induced arthritis. Arthritis Res Ther 17:169 (2015). IF . PubMed: 26104678
- Muschter D et al. Sympathetic Neurotransmitters Modulate Osteoclastogenesis and Osteoclast Activity in the Context of Collagen-Induced Arthritis. PLoS One 10:e0139726 (2015). IF . PubMed: 26431344
- Wasserman DI et al. Cholinergic control of morphine-induced locomotion in rostromedial tegmental nucleus versus ventral tegmental area sites. Eur J Neurosci 38:2774-85 (2013). IHC-FoFr ; Mouse . PubMed: 23773170