Anti-Muscarinic Acetylcholine Receptor M4/CHRM4抗体[18C7.2] (ab77956)
![Flow Cytometry - Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody [18C7.2] (ab77956)](https://www.abcam.cn/ps/products/77/ab77956/Images/Muscarinic-Acetylcholine-Receptor-M4-Primary-antibodies-ab77956-6.jpg "Flow Cytometry - Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody [18C7.2] (ab77956)")
Key features and details
- Mouse monoclonal [18C7.2] to Muscarinic Acetylcholine Receptor M4/CHRM4
- Suitable for: IHC-Fr, IP, Flow Cyt, WB
- Reacts with: Mouse, Rat, Human, Monkey
- Isotype: IgG1
概述
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产品名称
Anti-Muscarinic Acetylcholine Receptor M4/CHRM4抗体[18C7.2]
参阅全部 Muscarinic Acetylcholine Receptor M4/CHRM4 一抗 -
描述
小鼠单克隆抗体[18C7.2] to Muscarinic Acetylcholine Receptor M4/CHRM4 -
宿主
Mouse -
特异性
ab77956 reacts with Muscarinic Acetylcholine Receptor M4/CHRM4. There is no reactivity with the other subtypes.
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经测试应用
适用于: IHC-Fr, IP, Flow Cyt, WBmore details -
种属反应性
与反应: Mouse, Rat, Human, Monkey -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Transfected CHO cell lines
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.1% Sodium azide
Constituents: 1.45% Sodium chloride, 0.0536% PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Purified from TCS. -
克隆
单克隆 -
克隆编号
18C7.2 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77956于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
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| WB | (2) |
Use at an assay dependent concentration.
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| 说明 |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. |
靶标
- Information by UniProt
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数据库链接
- Entrez Gene: 1132 Human
- Entrez Gene: 12672 Mouse
- Entrez Gene: 25111 Rat
- Omim: 118495 Human
- SwissProt: P08173 Human
- SwissProt: P32211 Mouse
- SwissProt: P08485 Rat
- Unigene: 248100 Human
see all -
别名
- AChR antibody
- ACM4_HUMAN antibody
- Cholinergic receptor muscarinic 4 antibody
see all
图片
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Flow Cytometry - Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody [18C7.2] (ab77956)
Overlay histogram showing SH-SY5Y cells stained with ab77956 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77956, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab77956 被引用在 6 文献中.
- Tarafder S et al. A Combination of Oxo-M and 4-PPBP as a potential regenerative therapeutics for tendon injury. Theranostics 9:4241-4254 (2019). PubMed: 31281545
- Thomson SR et al. Cell-Type-Specific Translation Profiling Reveals a Novel Strategy for Treating Fragile X Syndrome. Neuron 95:550-563.e5 (2017). PubMed: 28772121
- Wasserman DI et al. Muscarinic control of rostromedial tegmental nucleus GABA neurons and morphine-induced locomotion. Eur J Neurosci 44:1761-70 (2016). PubMed: 26990801
- Mei F et al. Accelerated remyelination during inflammatory demyelination prevents axonal loss and improves functional recovery. Elife 5:N/A (2016). IHC-FoFr ; Mouse . PubMed: 27671734
- Zhao Z et al. Involvement of HCN Channel in Muscarinic Inhibitory Action on Tonic Firing of Dorsolateral Striatal Cholinergic Interneurons. Front Cell Neurosci 10:71 (2016). IHC . PubMed: 27047336
- Yokoi F et al. Decreased dopamine receptor 1 activity and impaired motor-skill transfer in Dyt1 ?GAG heterozygous knock-in mice. Behav Brain Res 279:202-10 (2015). PubMed: 25451552