重组Anti-Muscarinic Acetylcholine Receptor 2/CM2抗体[EPR4568] (ab109226)
![Western blot - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab109226)](https://www.abcam.cn/ps/products/109/ab109226/Images/ab109226-121890-anti-muscarinic-acetylcholine-receptor-2-antibody-epr4568-western-blot.jpg "Western blot - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab109226)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4568] to Muscarinic Acetylcholine Receptor 2/CM2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Muscarinic Acetylcholine Receptor 2/CM2抗体[EPR4568]
参阅全部 Muscarinic Acetylcholine Receptor 2/CM2 一抗 -
描述
兔单克隆抗体[EPR4568] to Muscarinic Acetylcholine Receptor 2/CM2 -
宿主
Rabbit -
特异性
Please note: blast analysis of the immunogen sequence for ab109226 shows 64% homology with the Muscarinic Acetylcholine Receptor 3, therefore some reactivity with this receptor subtype may be possible. -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: SH-SY5Y, fetal brain, U87-MG, Neuro-2a, and Y79 lysates IHC-P: Human brain tissue IF: SH-SY5Y cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Frozen Stock (-20C). Shelf life 12 months. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4568 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] - BSA and Azide free (ab247798)
- PE Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab318371)
- APC Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab318474)
- Alexa Fluor® 488 Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab318577)
- Alexa Fluor® 647 Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab318680)
- Alexa Fluor® 594 Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab318783)
- Alexa Fluor® 555 Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab318883)
- Alexa Fluor® 750 Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab320937)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109226于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
|
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| IHC-P | (1) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
应用说明
Is unsuitable for Flow Cyt.
靶标
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功能
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. -
疾病相关
Genetic variations in CHRM2 can influence susceptibility to major depressive disorder (MDD) [MIM:608516]. MDD is one of the most common psychiatric disorders. MDD is a complex trait characterized by one or more major depressive episodes without a history of manic, mixed, or hypomanic episodes. A major depressive episode is characterized by at least 2 weeks during which there is a new onset or clear worsening of either depressed mood or loss of interest or pleasure in nearly all activities. Four additional symptoms must also be present including changes in appetite, weight, sleep, and psychomotor activity; decreased energy; feelings of worthlessness or guilt; difficulty thinking, concentrating, or making decisions; or recurrent thoughts of death or suicidal ideation, plans, or attempts. The episode must be accompanied by distress or impairment in social, occupational, or other important areas of functioning. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily. -
细胞定位
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1129 Human
- Entrez Gene: 243764 Mouse
- Entrez Gene: 81645 Rat
- Omim: 118493 Human
- SwissProt: P08172 Human
- SwissProt: Q9ERZ4 Mouse
- SwissProt: P10980 Rat
- Unigene: 535891 Human
see all -
别名
- 7TM receptor antibody
- Acetylcholine receptor, muscarinic, 2 antibody
- AChR antibody
see all
图片
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Western blot - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab109226)All lanes : Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab109226) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : Fetal brain tissue lysate
Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 4 : Neuro-2a lysate
Lane 5 : Y79 (Human retinoblastoma cell line) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab109226)](https://www.abcam.cn/ps/products/109/ab109226/Images/ab109226-121889-anti-muscarinic-acetylcholine-receptor-2-antibody-epr4568-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab109226)Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109226 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab109226)](https://www.abcam.cn/ps/products/109/ab109226/Images/ab109226-3-benefits-of-recombinant-antibodies.png)
Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [EPR4568] (ab109226)
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab109226 被引用在 11 文献中.
- Wu Q et al. Cholinergic drugs reduce metabolic inflammation and diabetic myocardial injury by regulating the gut bacterial component lipopolysaccharide-induced ERK/Egr-1 pathway. FASEB J 37:e22917 (2023). PubMed: 37039813
- Tanaka-Kanegae R & Hamada K A novel in vitro assay model developed to measure both extracellular and intracellular acetylcholine levels for screening cholinergic agents. PLoS One 16:e0258420 (2021). PubMed: 34637466
- He W et al. Changes in the expression and function of the PDE5 pathway in the obstructed urinary bladder. J Cell Mol Med 24:13181-13195 (2020). PubMed: 33009887
- Bachmann SB et al. A Distinct Role of the Autonomic Nervous System in Modulating the Function of Lymphatic Vessels under Physiological and Tumor-Draining Conditions. Cell Rep 27:3305-3314.e13 (2019). PubMed: 31189113
- Kashyap MP et al. BDNF overexpression in the bladder induces neuronal changes to mediate bladder overactivity. Am J Physiol Renal Physiol 315:F45-F56 (2018). PubMed: 29092846