Anti-Muscarinic Acetylcholine Receptor 1/CHRM1抗体(ab77098)
Key features and details
- Goat polyclonal to Muscarinic Acetylcholine Receptor 1/CHRM1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-Muscarinic Acetylcholine Receptor 1/CHRM1抗体 -
描述
山羊多克隆抗体to Muscarinic Acetylcholine Receptor 1/CHRM1 -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Dog, Pig, Rhesus monkey, Orangutan
-
免疫原
Synthetic peptide corresponding to Human Muscarinic Acetylcholine Receptor 1/CHRM1 aa 438-453 (C terminal).
Sequence:C-DKRRWRKIPKRPGSVH
Database link: NP_000729.2 -
阳性对照
- Human brain lysate.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab77098 is purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77098于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 51 kDa.
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
WB
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 51 kDa. 1 hour primary incubation is recommended for this product. |
靶标
-
相关性
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. Muscarinic receptors are so called as they are more sensitive to the effects of the alkaloid muscarine, rather than nicotine. -
细胞定位
Cell Membrane; multi-pass membrane protein -
数据库链接
- Entrez Gene: 1128 Human
- Entrez Gene: 12669 Mouse
- Entrez Gene: 397099 Pig
- Entrez Gene: 25229 Rat
- Omim: 118510 Human
- SwissProt: P11229 Human
- SwissProt: P12657 Mouse
- SwissProt: Q5R949 Orangutan
see all -
别名
- AChR antibody
- Cholinergic receptor muscarinic 1 antibody
- CHRM1 antibody
see all
图片
-
Western blot - Anti-Muscarinic Acetylcholine Receptor 1/CHRM1 antibody (ab77098)Anti-Muscarinic Acetylcholine Receptor 1/CHRM1 antibody (ab77098) at 0.300000011920929 µg/ml + human brain lysate (in RIPA buffer) at 35 µg
Predicted band size: 51 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
数据表及文件
-
Datasheet download
文献 (4)
ab77098 被引用在 4 文献中.
- Saha S et al. IMPA1 dependent regulation of phosphatidylinositol 4,5-bisphosphate and calcium signalling by lithium. Life Sci Alliance 7:N/A (2024). PubMed: 38056909
- Tsolias A & Medalla M Muscarinic Acetylcholine Receptor Localization on Distinct Excitatory and Inhibitory Neurons Within the ACC and LPFC of the Rhesus Monkey. Front Neural Circuits 15:795325 (2021). PubMed: 35087381
- Dankis M et al. Novel Insights Into Muscarinic and Purinergic Responses in Primary Cultures of Rat Lacrimal Gland Myoepithelial Cells. Invest Ophthalmol Vis Sci 62:19 (2021). PubMed: 34546325
- Winder M et al. Autonomic Receptor-mediated Regulation of Production and Release of Nitric Oxide in Normal and Malignant Human Urothelial Cells. Basic Clin Pharmacol Toxicol 121:257-265 (2017). PubMed: 28437032
