Anti-Musashi 1 / Msi1抗体[Y2709] (ab129819)
Key features and details
- Mouse monoclonal [Y2709] to Musashi 1 / Msi1
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG2a
概述
-
产品名称
Anti-Musashi 1 / Msi1抗体[Y2709]
参阅全部 Musashi 1 / Msi1 一抗 -
描述
小鼠单克隆抗体[Y2709] to Musashi 1 / Msi1 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
corresponding to Musashi 1/ Msi1.
-
阳性对照
- WB: human brain membrane; E14 rat embryonic brain; SHSY5Y whole cell; 46C whole cell. ICC/IF: HAP1-MSI1 cells
-
常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
-
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
Y2709 -
同种型
IgG2a -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129819于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
|
|
ICC/IF |
Use a concentration of 5 µg/ml.
|
说明 |
---|
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa). |
ICC/IF
Use a concentration of 5 µg/ml. |
靶标
-
功能
RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system. -
组织特异性
Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines. -
序列相似性
Belongs to the Musashi family.
Contains 2 RRM (RNA recognition motif) domains. -
结构域
The first RNA recognition motif binds more strongly to RNA compared to the second one. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 4440 Human
- Entrez Gene: 17690 Mouse
- Entrez Gene: 259272 Rat
- Omim: 603328 Human
- SwissProt: O43347 Human
- SwissProt: Q61474 Mouse
- SwissProt: Q8K3P4 Rat
- Unigene: 158311 Human
see all -
别名
- Msi 1 antibody
- Msi1 antibody
- MSI1H_HUMAN antibody
see all
图片
-
ab129819 staining Musashi 1 / Msi1 in HAP1-MSI1 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab129819 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
-
All lanes : Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819) at 5 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : MSI1 knockout HAP1 whole cell lysate
Lane 3 : SH-SY5Y whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 39 kDa
Observed band size: 39 kDaLanes 1 - 3: Merged signal (red and green). Green - ab129819 observed at 39 kDa. Red - loading control, ab52866, observed at 50 kDa.
ab129819 was shown to specifically react with Musashi 1 / Msi1 in wild-type HAP1 cells as signal was lost in MSI1 knockout cells. Wild-type and MSI1 knockout samples were subjected to SDS-PAGE. Ab129819 and ab52866 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 5 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
All lanes : Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819) at 5 µg/ml
Lane 1 : Brain (Human) Membrane Lysate - fetal normal tissue (ab29457)
Lane 2 : E14 Rat Embryo Brain Tissue Lysate
Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 4 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutes
数据表及文件
-
Datasheet download
文献 (2)
ab129819 被引用在 2 文献中.
- Qian C et al. Neural stem cell‑derived exosomes transfer miR‑124‑3p into cells to inhibit glioma growth by targeting FLOT2. Int J Oncol 61:N/A (2022). PubMed: 35929514
- Kuwako K et al. Neural RNA-binding protein Musashi1 controls midline crossing of precerebellar neurons through posttranscriptional regulation of Robo3/Rig-1 expression. Neuron 67:407-21 (2010). IP . PubMed: 20696379