Anti-Mus81抗体[MTA30 2G10/3] (ab14387)
![Western blot - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)](https://www.abcam.cn/ps/products/14/ab14387/Images/ab14387-21896-anti-mus81-antibody-mta30-2g10-3-western-blot.jpg "Western blot - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)")
Key features and details
- Mouse monoclonal [MTA30 2G10/3] to Mus81
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
概述
-
产品名称
Anti-Mus81抗体[MTA30 2G10/3]
参阅全部 Mus81 一抗 -
描述
小鼠单克隆抗体[MTA30 2G10/3] to Mus81 -
宿主
Mouse -
经测试应用
适用于: WB, Flow Cytmore details -
种属反应性
与反应: Human
不与反应: Mouse -
免疫原
Mus81 protein.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
from TCS -
克隆
单克隆 -
克隆编号
MTA30 2G10/3 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG2a -
研究领域
相关产品
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab14387于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (7) |
1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 66 kDa).
Tested on both recombinant protein and whole cell extract. |
| Flow Cyt |
Use 0.5µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 66 kDa). Tested on both recombinant protein and whole cell extract. |
|
Flow Cyt
Use 0.5µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
靶标
-
功能
Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the XPF family.
Contains 1 ERCC4 domain. -
发展阶段
Expressed in S phase and G2 phase. -
细胞定位
Nucleus > nucleolus. Recruited to foci of DNA damage in S-phase cells. - Information by UniProt
-
数据库链接
- Entrez Gene: 80198 Human
- Omim: 606591 Human
- SwissProt: Q96NY9 Human
- Unigene: 288798 Human
-
别名
- Crossover junction endonuclease MUS81 antibody
- FLJ21012 antibody
- FLJ44872 antibody
see all
图片
-
Western blot - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)Anti-Mus81 antibody [MTA30 2G10/3] (ab14387) at 1/1000 dilution + HeLa cell lysate
Predicted band size: 66 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted? -
![Flow Cytometry - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)](https://www.abcam.cn/ps/products/14/ab14387/Images/ab14387-21895-anti-mus81-antibody-mta30-2g10-3-flow-cytometry.jpg)
Flow Cytometry - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)Overlay histogram showing HeLa cells stained with ab14387 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14387, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (59)
ab14387 被引用在 59 文献中.
- Benitez A et al. GEN1 promotes common fragile site expression. Cell Rep 42:112062 (2023). PubMed: 36729836
- Broderick R et al. Pathway choice in the alternative telomere lengthening in neoplasia is dictated by replication fork processing mediated by EXD2's nuclease activity. Nat Commun 14:2428 (2023). PubMed: 37105990
- Marini V et al. MUS81 cleaves TOP1-derived lesions and other DNA-protein cross-links. BMC Biol 21:110 (2023). PubMed: 37194054
- Moore CE et al. RFWD3 promotes ZRANB3 recruitment to regulate the remodeling of stalled replication forks. J Cell Biol 222:N/A (2023). PubMed: 37036693
- Mehta KPM et al. CHK1 phosphorylates PRIMPOL to promote replication stress tolerance. Sci Adv 8:eabm0314 (2022). PubMed: 35353580