Anti-MURF1抗体[7B8E1] (ab201941)
Key features and details
- Mouse monoclonal [7B8E1] to MURF1
- Suitable for: ICC/IF, Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-MURF1抗体[7B8E1]
参阅全部 MURF1 一抗 -
描述
小鼠单克隆抗体[7B8E1] to MURF1 -
宿主
Mouse -
经测试应用
适用于: ICC/IF, Flow Cyt, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human MURF1 aa 250-350.
Database link: Q969Q1 -
阳性对照
- HeLa cells; Human rectum cancer and muscle tissues.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
7B8E1 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab201941于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/200 - 1/1000.
|
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Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IHC-P |
1/200 - 1/1000.
|
说明 |
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ICC/IF
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P
1/200 - 1/1000. |
靶标
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功能
E3 ubiquitin ligase. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells. -
组织特异性
Muscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 1 B box-type zinc finger.
Contains 1 COS domain.
Contains 1 RING-type zinc finger. -
发展阶段
Expressed throughout all developmental stages. -
结构域
The RING-type zinc finger mediates interaction with SUMO2 and localization to the nucleus. Also required for the E3 ubiquitin ligase activity. -
细胞定位
Cytoplasm. Nucleus. Localizes to the M- and Z-lines in skeletal muscle. Colocalizes with TNNI3 in myocytes. - Information by UniProt
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数据库链接
- Entrez Gene: 84676 Human
- Entrez Gene: 433766 Mouse
- Entrez Gene: 140939 Rat
- Omim: 606131 Human
- SwissProt: Q969Q1 Human
- SwissProt: Q38HM4 Mouse
- SwissProt: Q91Z63 Rat
- Unigene: 279709 Human
see all -
别名
- E3 ubiquitin-protein ligase TRIM63 antibody
- FLJ32380 antibody
- IRF antibody
see all
图片
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Immunofluorescent analysis of HeLa cells labeling MURF1 with ab201941 at 1/200 dilution. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
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Flow cytometric analysis of HeLa cells labeling MURF1 with ab201941 at 1/200 dilution (green) compared to a negative control (red).
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Immunohistochemical analysis of paraffin-embedded Human rectum cancer tissue labeling MURF1 with ab201941 at 1/200 dilution, with DAB staining.
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Immunohistochemical analysis of paraffin-embedded Human muscle tissue labeling MURF1 with ab201941 at 1/200 dilution, with DAB staining.
实验方案
数据表及文件
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Datasheet download
文献 (4)
ab201941 被引用在 4 文献中.
- Singh M et al. Hydrogen sulfide mitigates skeletal muscle mitophagy-led tissue remodeling via epigenetic regulation of the gene writer and eraser function. Physiol Rep 10:e15422 (2022). PubMed: 35986494
- Liu L et al. Formononetin ameliorates muscle atrophy by regulating myostatin-mediated PI3K/Akt/FoxO3a pathway and satellite cell function in chronic kidney disease. J Cell Mol Med 25:1493-1506 (2021). PubMed: 33405354
- Cao YY et al. Sepsis induces muscle atrophy by inhibiting proliferation and promoting apoptosis via PLK1-AKT signalling. J Cell Mol Med 25:9724-9739 (2021). PubMed: 34514712
- Kang SH et al. Forkhead box O3 plays a role in skeletal muscle atrophy through expression of E3 ubiquitin ligases MuRF-1 and atrogin-1 in Cushing's syndrome. Am J Physiol Endocrinol Metab 312:E495-E507 (2017). PubMed: 28246104