重组Anti-MURF1 + MURF3 + MURF2抗体[EPR6431(2)] (ab172479)
![Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)](https://www.abcam.cn/ps/products/172/ab172479/Images/ab172479-275257-anti-murf1-murf3-murf2-antibody-epr64312-western-blot.jpg "Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6431(2)] to MURF1 + MURF3 + MURF2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-MURF1 + MURF3 + MURF2抗体[EPR6431(2)] -
描述
兔单克隆抗体[EPR6431(2)] to MURF1 + MURF3 + MURF2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human MURF1 aa 1-100. The exact sequence is proprietary.
Database link: Q969Q1 -
阳性对照
- Rat muscle, Mouse muscle and Human skeletal muscle tissue lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6431(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab172479于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 40 kDa.
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| 说明 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 40 kDa. |
靶标
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细胞定位
MURF1: Cytoplasm. Nucleus. Localizes to the M- and Z-lines in skeletal muscle. Colocalizes with TNNI3 in myocytes. MURF3: Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Associates with microtubules. Localizes to the Z-lines in skeletal muscles. MURF2: Cytoplasm. Nucleus. Nuclear under atrophic conditions and upon mechanical signals. Localizes to the sarcomeric M-band in cardiomyocytes. Colocalizes in part with microtubules. -
数据库链接
- Entrez Gene: 57159 Human
- Entrez Gene: 84675 Human
- Entrez Gene: 84676 Human
- Entrez Gene: 381485 Mouse
- Entrez Gene: 433766 Mouse
- Entrez Gene: 58522 Mouse
- Entrez Gene: 140939 Rat
- Entrez Gene: 362708 Rat
see all
图片
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Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)All lanes : Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : Mouse muscle lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDaBlocking and diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)](https://www.abcam.cn/ps/products/172/ab172479/Images/ab172479-194774-anti-murf1-murf3-murf2-antibody-epr64312-western-blot.jpg)
Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)All lanes : Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution
Lane 1 : Rat muscle tissue lysate
Lane 2 : Mouse muscle tissue lysate
Lane 3 : Human skeletal muscle tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDa -
![Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)](https://www.abcam.cn/ps/products/172/ab172479/Images/ab172479-307527-anti-murf1-murf3-murf2-antibody-epr64312-western-blot.jpg)
Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution + His-Tagged human MURF1 (aa 1 to 353) recombinant protein at 1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 40 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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![Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)](https://www.abcam.cn/ps/products/172/ab172479/Images/ab172479-307528-anti-murf1-murf3-murf2-antibody-epr64312-western-blot.jpg)
Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution + His-Tagged human MURF3 (full length) recombinant protein at 1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 40 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
-
![Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)](https://www.abcam.cn/ps/products/172/ab172479/Images/ab172479-307532-anti-murf1-murf3-murf2-antibody-epr64312-western-blot.jpg)
Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/500 dilution + His-Tagged human MURF2 (full length) recombinant protein at 1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 40 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 150 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
-
![Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)](https://www.abcam.cn/ps/products/172/ab172479/Images/ab172479-275258-anti-murf1-murf3-murf2-antibody-epr64312-western-blot.jpg)
Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/5000 dilution + Mouse muscle lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 150 secondsBlocking and diluting buffer: 5% NFDM/TBST
-
![Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)](https://www.abcam.cn/ps/products/172/ab172479/Images/ab172479-7-benefits-of-recombinant-antibodies.png)
Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
数据表及文件
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SDS download
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Datasheet download
文献 (60)
ab172479 被引用在 60 文献中.
- Yeon MH et al. Bavachin and Corylifol A Improve Muscle Atrophy by Enhancing Mitochondria Quality Control in Type 2 Diabetic Mice. Antioxidants (Basel) 12:N/A (2023). PubMed: 36671000
- Park E et al. The Inhibition of Autophagy and Pyroptosis by an Ethanol Extract of Nelumbo nucifera Leaf Contributes to the Amelioration of Dexamethasone-Induced Muscle Atrophy. Nutrients 15:N/A (2023). PubMed: 36839161
- Zhang L et al. Immunoproteasome subunit β5i promotes perifascicular muscle atrophy in dermatomyositis by upregulating RIG-I. RMD Open 9:N/A (2023). PubMed: 36854567
- Tao W et al. Ursolic Acid Alleviates Cancer Cachexia and Prevents Muscle Wasting via Activating SIRT1. Cancers (Basel) 15:N/A (2023). PubMed: 37190306
- Kimira Y et al. Preventive Effects of Collagen-Derived Dipeptide Prolyl-Hydroxyproline against Dexamethasone-Induced Muscle Atrophy in Mouse C2C12 Skeletal Myotubes. Biomolecules 13:N/A (2023). PubMed: 38002299