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Neuroscience Neurotransmission Secretory Vesicles Munc18

Anti-Munc18-1抗体(ab3451)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (13)

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Western blot - Anti-Munc18-1 antibody (ab3451)
  • Immunocytochemistry - Anti-Munc18-1 antibody (ab3451)
  • Western blot - Anti-Munc18-1 antibody (ab3451)
  • Western blot - Anti-Munc18-1 antibody (ab3451)
  • Western blot - Anti-Munc18-1 antibody (ab3451)

Key features and details

  • Rabbit polyclonal to Munc18-1
  • Suitable for: WB, ICC
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Munc18-1 antibody [EPR4849] (ab109023)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Munc18-1抗体
    参阅全部 Munc18-1 一抗
  • 描述

    兔多克隆抗体to Munc18-1
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, ICCmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Cow, Pig
  • 免疫原

    Synthetic peptide corresponding to Human Munc18-1 aa 58-70.
    Sequence:

    VEDINKRREPIPS


    (Peptide available as ab4982)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • WB: Mouse and rat brain tissue lysates ICC: U-87 MG cells
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • Munc18
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
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    • Metabolic signaling pathways
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    • Integration of energy
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    • Neuroscience
    • Processes

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3451于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (2)
Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa.

By Western blot, this antibody detects an ~67 kDa protein representing Munc-18 from rat whole brain extract.

ICC
Use a concentration of 2 µg/ml.
说明
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa.

By Western blot, this antibody detects an ~67 kDa protein representing Munc-18 from rat whole brain extract.

ICC
Use a concentration of 2 µg/ml.

靶标

  • 功能

    May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions.
  • 组织特异性

    Brain and spinal cord. Highly enriched in axons.
  • 疾病相关

    Defects in STXBP1 are the cause of epileptic encephalopathy early infantile type 4 (EIEE4) [MIM:612164]. Affected individuals have neonatal or infantile onset of seizures, suppression-burst pattern on EEG, profound mental retardation, and MRI evidence of hypomyelination.
  • 序列相似性

    Belongs to the STXBP/unc-18/SEC1 family.
  • 细胞定位

    Cytoplasm. Membrane.
  • Target information above from: UniProt accession P61764 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 404293 Chicken
    • Entrez Gene: 282378 Cow
    • Entrez Gene: 6812 Human
    • Entrez Gene: 20910 Mouse
    • Entrez Gene: 25558 Rat
    • Omim: 602926 Human
    • SwissProt: Q6R748 Chicken
    • SwissProt: P61763 Cow
    • SwissProt: P61764 Human
    • SwissProt: O08599 Mouse
    • SwissProt: P61765 Rat
    • Unigene: 288229 Human
    • Unigene: 278865 Mouse
    • Unigene: 80843 Rat
    see all
  • 别名

    • FLJ37475 antibody
    • Munc 18 1 antibody
    • Munc 18a antibody
    • MUNC18 1 antibody
    • N-Sec1 antibody
    • Neuronal SEC1 antibody
    • NSec1 antibody
    • p67 antibody
    • Protein unc-18 homolog 1 antibody
    • Protein unc-18 homolog A antibody
    • Rb sec1 antibody
    • RBSEC1 antibody
    • STXB1_HUMAN antibody
    • STXBP1 antibody
    • Syntaxin binding protein 1 antibody
    • Syntaxin-binding protein 1 antibody
    • Unc 18 homolog antibody
    • Unc 18A antibody
    • Unc-18A antibody
    • Unc18 1 antibody
    • UNC18 antibody
    • Unc18-1 antibody
    see all

图片

  • Western blot - Anti-Munc18-1 antibody (ab3451)
    Western blot - Anti-Munc18-1 antibody (ab3451)
    Anti-Munc18-1 antibody (ab3451) at 2 µg/ml + Mouse brain tissue lysate at 30 µg

    Secondary
    Goat anti-Rabbit IgG (H+L) Superclonal™ at 1/4000 dilution

    Observed band size: 67 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry - Anti-Munc18-1 antibody (ab3451)
    Immunocytochemistry - Anti-Munc18-1 antibody (ab3451)

    Immunocytochemistry analysis of 70% confluent log phase U-87 MG cells labeling Munc18-1 with ab3451 at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

  • Western blot - Anti-Munc18-1 antibody (ab3451)
    Western blot - Anti-Munc18-1 antibody (ab3451)
    All lanes : Anti-Munc18-1 antibody (ab3451) at 2 µg/ml

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate
    Lane 3 : Rat liver tissue lysate
    Lane 4 : Rat kidney tissue lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ at 1/4000 dilution

    Observed band size: 67 kDa why is the actual band size different from the predicted?



    Detected by chemiluminescence

  • Western blot - Anti-Munc18-1 antibody (ab3451)
    Western blot - Anti-Munc18-1 antibody (ab3451)This image is courtesy of an anonymous Abreview
    Anti-Munc18-1 antibody (ab3451) at 1/5000 dilution + Mouse hippocampal homogenate at 5 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97069) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 67 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute

    See Abreview

  • Western blot - Anti-Munc18-1 antibody (ab3451)
    Western blot - Anti-Munc18-1 antibody (ab3451)

    Western blot of Munc-18 in rat brain homogenate immunoprecipitated with ab3451.
    1. Extract only.
    2-4. 750 ug of extract immunoprecipitated with 1 ug, 5 ug, and 10 ug of ab3451, respectively.

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (13)

发表研究结果有使用 ab3451?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab3451 被引用在 13 文献中.

  • Tang F  et al. Munc18-1 Contributes to Hippocampal Injury in Septic Rats Through Regulation of Syntanxin1A and Synaptophysin and Glutamate Levels. Neurochem Res 48:791-803 (2023). PubMed: 36335177
  • Lu Z  et al. Base-edited cynomolgus monkeys mimic core symptoms of STXBP1 encephalopathy. Mol Ther 30:2163-2175 (2022). PubMed: 35283272
  • Wang CC  et al. Presynaptic Short-Term Plasticity Persists in the Absence of PKC Phosphorylation of Munc18-1. J Neurosci 41:7329-7339 (2021). PubMed: 34290081
  • Ishii C  et al. CAPS1 is involved in hippocampal synaptic plasticity and hippocampus-associated learning. Sci Rep 11:8656 (2021). PubMed: 33883618
  • Di Marco Vieira B  et al. Extracellular Alpha-Synuclein Promotes a Neuroinhibitory Secretory Phenotype in Astrocytes. Life (Basel) 10:N/A (2020). PubMed: 32911644
View all Publications for this product

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Munc18-1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing (10% separating gel)
Sample
Mouse Tissue lysate - whole (Hippocampal homogenate)
Specification
Hippocampal homogenate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 12 2014

Western blot abreview for Anti-Munc18-1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Hippocampus)
Loading amount
30 µg
Specification
Hippocampus
Gel Running Conditions
Reduced Denaturing (12% TGS)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 20 2008

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