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Anti-Mumps nucleoprotein抗体[8H4] (ab9876)

Submit a review Q&A (1)References (8)
Immunocytochemistry/ Immunofluorescence - Anti-Mumps nucleoprotein antibody [8H4] (ab9876)

    Key features and details

    • Mouse monoclonal [8H4] to Mumps nucleoprotein
    • Suitable for: ICC/IF
    • Isotype: IgG2b

    概述

    • 产品名称

      Anti-Mumps nucleoprotein抗体[8H4]
      参阅全部 Mumps nucleoprotein 一抗

    • 描述

      小鼠单克隆抗体[8H4] to Mumps nucleoprotein

    • 宿主

      Mouse

    • 特异性

      Recognises recombinant yeast-derived mumps NP. Recognises native NP in mumps virus infected Vero cells. This antibody has been tested on 3 different mumps virus strains isolated in the UK: 55799-98, SJ801-95 and Gloucester.

    • 经测试应用

      适用于: ICC/IFmore details

    • 种属反应性

      与反应: Mumps virus

    • 免疫原

      Full length protein corresponding to Mumps nucleoprotein aa 1 to the C-terminus.
      Database link: AAG37826.1

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 常规说明

      This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • 存储溶液

      pH: 6.80
      Preservative: 0.1% Sodium azide
      Constituent: 15% Fetal Bovine Serum (FBS)
    • Concentration information loading...

    • 纯度

      Tissue culture supernatant

    • 克隆

      单克隆

    • 克隆编号

      8H4

    • 骨髓瘤

      NS0/1

    • 同种型

      IgG2b

    • 轻链类型

      unknown

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab9876于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    ICC/IF
    1/1 - 1/20.
    说明
    ICC/IF
    1/1 - 1/20.

    靶标

    • 别名

      • NP antibody
      • Nucleocapsid protein antibody
      • Nucleoprotein antibody

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-Mumps nucleoprotein antibody [8H4] (ab9876)
      Immunocytochemistry/ Immunofluorescence - Anti-Mumps nucleoprotein antibody [8H4] (ab9876)

      Immunofluorescence staining of mumps virus infected Vero cells with monoclonal antibody 8H4.



      Immunofluorescence staining of mumps virus infected Vero cells with monoclonal antibody 8H4.

    文献 (8)

    发表研究结果有使用 ab9876？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab9876 被引用在 8 文献中.

    • Zhang X  et al. Molecular mechanisms of stress-induced reactivation in mumps virus condensates. Cell 186:1877-1894.e27 (2023). PubMed: 37116470
    • Hao X  et al. Development of Improved Mumps Vaccine Candidates by Mutating Viral mRNA Cap Methyltransferase Sites in the Large Polymerase Protein. Virol Sin 36:521-536 (2021). PubMed: 33284397
    • Wu H  et al. Mumps virus infection disrupts blood-testis barrier through the induction of TNF-a in Sertoli cells. FASEB J 33:12528-12540 (2019). PubMed: 31450968
    • Wu H  et al. Mouse Testicular Cell Type-Specific Antiviral Response against Mumps Virus Replication. Front Immunol 8:117 (2017). PubMed: 28239382
    • Jiang Q  et al. C-X-C motif chemokine ligand 10 produced by mouse Sertoli cells in response to mumps virus infection induces male germ cell apoptosis. Cell Death Dis 8:e3146 (2017). Mouse . PubMed: 29072682
    • Wu H  et al. Mumps virus-induced innate immune responses in mouse Sertoli and Leydig cells. Sci Rep 6:19507 (2016). PubMed: 26776505
    • Ma TM  et al. Serine racemase regulated by binding to stargazin and PSD-95: potential N-methyl-D-aspartate-a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (NMDA-AMPA) glutamate neurotransmission cross-talk. J Biol Chem 289:29631-41 (2014). WB . PubMed: 25164819
    • Samuel D  et al. High level expression of recombinant mumps nucleoprotein in Saccharomyces cerevisiae and its evaluation in mumps IgM serology. J Med Virol 66:123-30 (2002). PubMed: 11748668

    客户评价及客户问答

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    Question

    Please provide details of the stains of mumps virus that Abcam mumps antibodies have been tested on.

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    Answer

    Thank you for your recent telephone enquiry. I can confirm that ab9879,ab9880 and ab9392 have been created using nuclear protein from Recombinant mumps NP.Gloucester strain (Glouc 1/UK96). Antibodies ab9876 and ab9878 have been raised using recombiant NP protein. I am unable to find data on which strain this is taken from. I can confirm all of these antibodies (ab9392,ab9876, ab9878, ab9879, ab9880) have also been succesfully tested in mumps strains 55799-98 and SJ801-95. I can suggest checking the sequence alignment of the NP protein of the different strains you are interested in. We recommned that sequence homology should be over 85% to predict an antibody will detect a protein. Mumps antibody ab20859 has been tested sucessfully on the Enders strain (from which the immunogen was taken) and a CDC strain #V5-004. Other strains (including 55799-98 and SJ801-95 and Gloucester) have not been tested, including 55799-98 and SJ801-95 and Gloucester. Again, I can suggest conducting a sequence alignment search to see which strains may be detectable. I hope this information is helpful. Should you require any further assistance, please do not hesitate to contact us again.

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