AB24070

Anti-Mucin 5AC 抗体 [1-13M1]

Name: Mucin 5AC抗体[1-13M1] (ab24070)| Abcam中文官网
Brand: Abcam Limited
SKU: AB24070
Price: 4959 CNY
Availability: InStock

Anti-Mucin 5AC antibody [1-13M1]

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(20 Publications)

Mouse Monoclonal Mucin 5AC antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 20 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human MUC5AC.

查看别名

MUC5, MUC5AC, Mucin-5AC, MUC-5AC, Gastric mucin, Major airway glycoprotein, Tracheobronchial mucin, TBM

1 Images

Flow Cytometry - Anti-Mucin 5AC antibody [1-13M1] (AB24070)

Flow Cyt

Unknown

Flow Cytometry - Anti-Mucin 5AC antibody [1-13M1] (AB24070)

Overlay histogram showing A549 cells stained with ab24070 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24070, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

1-13M1

亚型

IgG1

不含载体蛋白

反应种属

Human

应用

Flow Cyt

applications

免疫原

Native Full Length Protein corresponding to Human MUC5AC.

P98088

表位

The epitope is destroyed by beta-mercaptoethanol.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein G

存储溶液

pH: 7.3 Preservative: 0.05% Sodium azide Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Mucin 5AC also known simply as MUC5AC is a high molecular weight glycoprotein with a mass in the megadaltons typically found in the human respiratory gastrointestinal and reproductive tracts. It is a major component of mucus which plays a critical role in protecting and lubricating the epithelial surfaces of these organs. MUC5AC is primarily expressed in the goblet cells of the respiratory epithelium and the stomach lining. Due to its significance researchers often use a MUC5AC ELISA or other detection methods such as tagging with Alexa Fluor 555 to measure its levels in various tissues.

Biological function summary

The functions of MUC5AC are central to the maintenance of the protective mucus barrier on epithelial surfaces preventing pathogen invasion and facilitating the clearance of inhaled particles. It is a part of the gel-forming mucin family and often forms complex structures by polymerizing with other mucins like MUC2 and MUC5B contributing to the viscoelastic properties of mucus. These interactions ensure the formation of a stable and robust mucosal barrier.

Pathways

The regulation of MUC5AC production involves several signaling pathways including the EGFR (epidermal growth factor receptor) pathway and the IL-13/STAT6 pathway. These pathways influence mucin production and secretion particularly in response to growth factors and inflammatory cytokines. Proteins such as 13M1 and 11M1 interact with MUC5AC within these pathways modulating its expression and function. This regulation is essential for normal mucus homeostasis and response to environmental challenges.

MUC5AC has a strong association with respiratory conditions like asthma and chronic obstructive pulmonary disease (COPD). In these diseases the overproduction or altered composition of MUC5AC contributes to airway obstruction and reduced pulmonary function. MUC5AC levels can be indicative of disease severity and are often measured via tests like the 5AC blood test. In these contexts proteins such as 5AC protein and 45M1 are often studied for their roles in facilitating disease progression and as potential therapeutic targets to modulate mucin levels in affected individuals.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by the mucociliary system (PubMed : 14535999, PubMed : 14718370). Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD) (PubMed : 14535999).

See full target information MUC5AC

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文献 (20)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 31: PubMed40211706

2025

Resveratrol inhibits lipopolysaccharide‑induced MUC5AC expression and airway inflammation via MAPK and Nrf2 pathways in human bronchial epithelial cells and an acute inflammatory mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaojuan Chen,Liutian Xie,Jianming Wang,Xiaoshan Su,Xiangjia Ye,Xiaoping Lin

The ocular surface 19:190-200 PubMed32502616

2020

Co-expression of SARS-CoV-2 entry genes in the superficial adult human conjunctival, limbal and corneal epithelium suggests an additional route of entry via the ocular surface.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph Collin,Rachel Queen,Darin Zerti,Birthe Dorgau,Maria Georgiou,Ivo Djidrovski,Rafiqul Hussain,Jonathan M Coxhead,Agatha Joseph,Paul Rooney,Steven Lisgo,Francisco Figueiredo,Lyle Armstrong,Majlinda Lako

Frontiers in bioengineering and biotechnology 8:190 PubMed32266229

2020

The Fabrication and Evaluation of Retinoic Acid-Loaded Electrospun Composite Biomaterials for Tracheal Tissue Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Cian O'Leary,Luis Soriano,Aidan Fagan-Murphy,Ivana Ivankovic,Brenton Cavanagh,Fergal J O'Brien,Sally-Ann Cryan

Mediators of inflammation 2019:3153240 PubMed32082074

2019

TGF-3 Induces Autophagic Activity by Increasing ROS Generation in a NOX4-Dependent Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Zhang,Hong-Mei Tang,Chun-Feng Liu,Xie-Fang Yuan,Xiao-Yun Wang,Ning Ma,Guo-Feng Xu,Song-Ping Wang,Jun Deng,Xing Wang

Frontiers in immunology 10:1682 PubMed31379870

2019

IL-19 Up-Regulates Mucin 5AC Production in Patients With Chronic Rhinosinusitis STAT3 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoping Lai,Xia Li,Lihong Chang,Xiaohong Chen,Zizhen Huang,Hongwei Bao,Jiancong Huang,Luoying Yang,Xifu Wu,Zhiyuan Wang,Joseph A Bellanti,Song Guo Zheng,Gehua Zhang

EBioMedicine 33:242-252 PubMed29997053

2018

TGF-β3 Promotes MUC5AC Hyper-Expression by Modulating Autophagy Pathway in Airway Epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Zhang,Hongmei Tang,Xiefang Yuan,Qin Ran,Xiaoyun Wang,Qi Song,Lei Zhang,Yuhuan Qiu,Xing Wang

The Journal of allergy and clinical immunology 135:395-406 PubMed25312757

2014

D prostanoid receptor 2 (chemoattractant receptor-homologous molecule expressed on TH2 cells) protein expression in asthmatic patients and its effects on bronchial epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sally E Stinson,Yassine Amrani,Christopher E Brightling

Investigative ophthalmology & visual science 55:6565-74 PubMed25212776

2014

DA-6034-induced mucin secretion via Ca2+-dependent pathways through P2Y receptor stimulation.

Applications

Species

Human

Hun Lee,Eung Kweon Kim,Ji Yeon Kim,Yu-Mi Yang,Dong Min Shin,Kyung Koo Kang,Tae-im Kim

Differentiation; research in biological diversity 87:119-26 PubMed24830354

2014

Branching morphogenesis of immortalized human bronchial epithelial cells in three-dimensional culture.

Applications

Unspecified application

Species

Unspecified reactive species

Aadil Kaisani,Oliver Delgado,Gail Fasciani,Sang Bum Kim,Woodring E Wright,John D Minna,Jerry W Shay

Toxicological sciences : an official journal of the Society of Toxicology 139:83-98 PubMed24496642

2014

Modulation of mucin mRNA (MUC5AC and MUC5B) expression and protein production and secretion in Caco-2/HT29-MTX co-cultures following exposure to individual and combined Fusarium mycotoxins.

Applications

Unspecified application

Species

Unspecified reactive species

Lam-Yim Murphy Wan,Kevin J Allen,Paul C Turner,Hani El-Nezami

View all publications

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Anti-Mucin 5AC 抗体 [1-13M1]

Anti-Mucin 5AC antibody [1-13M1]

Flow Cytometry - Anti-Mucin 5AC antibody [1-13M1] (AB24070)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

表位