Anti-MUC5B抗体[6F10-E4] (ab105460)
Key features and details
- Mouse monoclonal [6F10-E4] to MUC5B
- Suitable for: ELISA, WB
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-MUC5B抗体[6F10-E4]
参阅全部 MUC5B 一抗 -
描述
小鼠单克隆抗体[6F10-E4] to MUC5B -
宿主
Mouse -
特异性
Non glycosylated domain of mucin MUC5B. -
经测试应用
适用于: ELISA, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human MUC5B conjugated to keyhole limpet haemocyanin. Synthetic peptide from 4 of the cysteine domains of the tandem repeat region.
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常规说明
Detection of respiratory and salivary mucins in clinical and experimental material.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
纯度
Protein A purified -
Primary antibody说明
Detection of respiratory and salivary mucins in clinical and experimental material. -
克隆
单克隆 -
克隆编号
6F10-E4 -
骨髓瘤
Sp2 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab105460于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 596 kDa. PubMed: 14678646
Please consult PubMed ID 14678646 for the recommended electrophoresis conditions. |
| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 596 kDa. PubMed: 14678646 Please consult PubMed ID 14678646 for the recommended electrophoresis conditions. |
靶标
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功能
Gel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus. -
组织特异性
Expressed on surface airway epithelia. Expressed mainly in mucous cells of submucosal glands of airway tissues. Highly expressed in the sublingual gland. Also found in submaxillary glands, endocervix, gall bladder, and pancreas. -
序列相似性
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 3 HAT repeats.
Contains 3 TIL (trypsin inhibitory-like) domains.
Contains 3 VWFC domains.
Contains 4 VWFD domains. -
结构域
The cysteine residues in the Cys-rich subdomain repeats are not involved in disulfide bonding. -
翻译后修饰
Highly glycosylated. C-, N- and O-glycosylated. C-mannosylated in the Cys-rich subdomains probably on the first Trp residue of the WXXW motif. Highly O-glycosylated in the Ser/Thr-rich tandem repeat (TR) region. The repeat region is about 59% O-glycosylated with a high abundance of NeuAc(2)Hex(1)HexNac1-ol. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 727897 Human
- Omim: 600770 Human
- SwissProt: Q9HC84 Human
- Unigene: 523395 Human
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别名
- 2300002I04Rik antibody
- A130042M24 antibody
- AV085033 antibody
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实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab105460 被引用在 5 文献中.
- Saitou M et al. Functional Specialization of Human Salivary Glands and Origins of Proteins Intrinsic to Human Saliva. Cell Rep 33:108402 (2020). PubMed: 33207190
- Martinez-Valdebenito C et al. Characterization of Oral Immunity in Cases and Close Household Contacts Exposed to Andes Orthohantavirus (ANDV). Front Cell Infect Microbiol 10:557273 (2020). PubMed: 33224896
- van Leeuwen S et al. Early salivary changes in multiple myeloma patients undergoing autologous HSCT. Oral Dis 24:972-982 (2018). PubMed: 29637662
- Lachowicz-Scroggins ME et al. Abnormalities in MUC5AC and MUC5B Protein in Airway Mucus in Asthma. Am J Respir Crit Care Med 194:1296-1299 (2016). PubMed: 27845589
- Rousseau K et al. New monoclonal antibodies to non-glycosylated domains of the secreted mucins MUC5B and MUC7. Hybrid Hybridomics 22:293-9 (2003). WB, ELISA ; Human . PubMed: 14678646