重组Anti-MUC2抗体[EPR23479-88] - BSA and Azide free (Capture) (ab281261)
![Sandwich ELISA - Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture) (ab281261)](https://www.abcam.cn/ps/products/281/ab281261/Images/ab281261-2-human-muc2-elisa-kit-sandwich-elisa-standard-curve-2.jpg "Sandwich ELISA - Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture) (ab281261)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23479-88] to MUC2 - BSA and Azide free (Capture)
- Suitable for: IP, IHC-Fr, WB, Sandwich ELISA
- Reacts with: Rat, Human
概述
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产品名称
Anti-MUC2抗体[EPR23479-88] - BSA and Azide free (Capture)
参阅全部 MUC2 一抗 -
描述
兔单克隆抗体[EPR23479-88] to MUC2 - BSA and Azide free (Capture) -
宿主
Rabbit -
特异性
Unsuitable for rat IP.
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经测试应用
适用于: IP, IHC-Fr, WB, Sandwich ELISAmore details
不适用于: Flow Cyt (Intra) or ICC/IF -
种属反应性
与反应: Rat, Human
不与反应: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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常规说明
ab281261 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human MUC2. The recommended pair for sandwich ELISA is:
Capture: ab281261, Human MUC2 Capture Antibody (unconjugated)
Detector: ab281111, Human MUC2 Detector Antibody (unconjugated)
The reference range value is 78 - 5000 pg/mL.Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Constituent: 100% PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR23479-88 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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sELISA pair antibody
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab281261于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
1/50.
|
|
| IHC-Fr |
1/1000.
|
|
| WB |
1/1000. Predicted molecular weight: 540 kDa.
|
|
| Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795).
|
| 说明 |
|---|
|
IP
1/50. |
|
IHC-Fr
1/1000. |
|
WB
1/1000. Predicted molecular weight: 540 kDa. |
|
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795). |
靶标
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功能
Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer. -
组织特异性
Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder. -
序列相似性
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 TIL (trypsin inhibitory-like) domain.
Contains 2 VWFC domains.
Contains 4 VWFD domains. -
翻译后修饰
O-glycosylated.
May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.
At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway. -
细胞定位
Secreted. In the intestine, secreted into the inner and outer mucus layers. - Information by UniProt
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数据库链接
- Entrez Gene: 4583 Human
- Entrez Gene: 24572 Rat
- Omim: 158370 Human
- SwissProt: Q02817 Human
- SwissProt: Q62635 Rat
- Unigene: 315 Human
- Unigene: 195421 Rat
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别名
- Intestinal mucin 2 antibody
- Intestinal mucin-2 antibody
- MLP antibody
see all
图片
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Sandwich ELISA - Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture) (ab281261)Example of Human MUC2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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![Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture) (ab281261)](https://www.abcam.cn/ps/products/281/ab281261/Images/ab281261-1-RecombinantAntibodyBenefitfigure.png)
Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture) (ab281261)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab281261 尚未被引用在任何文献中。
