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AB281261

重组Anti-MUC2抗体[EPR23479-88] - BSA and Azide free (Capture)

Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal MUC2 antibody. Carrier free. Suitable for sELISA, WB, IHC-Fr, IP and reacts with Human, Rat samples.

查看别名

SMUC, MUC2, Mucin-2, MUC-2, Intestinal mucin-2

5 Images
Immunoprecipitation - Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture) (AB281261)
  • IP

Lab

Immunoprecipitation - Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture) (AB281261)

MUC2 was immunoprecipitated from 0.35 mg Human colon tissue lysate with ab281261 at 1/50 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab281261 at 1/1000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture) (ab281261) at 1/1000 dilution

Lane 1:

Human colon tissue lysate (Input) at 10 µg

Lane 2:

Human colon tissue lysate (+)

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab281261 in Human colon tissue lysate (-)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 140-170 kDa

false

Exposure time: 41s

Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture) (AB281261)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture) (AB281261)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh frozen) tissue labeling MUC2 with ab281261 at 1/1000 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/mL) dilution (Green).

Negative control : confocal image showing no staining on rat cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab281261 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/mL) dilution.

Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture) (AB281261)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture) (AB281261)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat colon (fresh frozen) tissue labeling MUC2 with ab281261 at 1/1000 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/mL) dilution (Green).

Confocal image showing positive staining on rat colon. The nuclear counterstain was DAPI (Blue). The section was incubated with ab281261 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/mL) dilution.

Western blot - Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture) (AB281261)
  • WB

Lab

Western blot - Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture) (AB281261)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture) (ab281261) at 1/1000 dilution

Lane 1:

Human colon tissue lysate at 10 µg

Lane 2:

Human small intestine tissue lysate at 10 µg

Lane 3:

Rat colon tissue lysate at 10 µg

Lane 4:

Rat small intestine tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 140-170 kDa

false

Exposure time: 26s

Sandwich ELISA - Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture) (AB281261)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture) (AB281261)

Example of Human MUC2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR23479-88

亚型

IgG

不含载体蛋白

Yes

反应种属

Human, Rat

应用

IHC-Fr, IP, WB, sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

Unsuitable for rat IP.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "sELISA-species-checked": "predicted", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/1000", "IHCFr-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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产品详情

ab281261 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human MUC2. The recommended pair for sandwich ELISA is:
Capture: ab281261, Human MUC2 Capture Antibody (unconjugated)
Detector: ab281111, Human MUC2 Detector Antibody (unconjugated)
The reference range value is 78 - 5000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Coats the epithelia of the intestines and other mucus membrane-containing organs to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces (PubMed : 17058067, PubMed : 19432394, PubMed : 33031746). Major constituent of the colon mucus, which is mainly formed by large polymeric networks of MUC2 secreted by goblet cells that cover the exposed surfaces of intestine (PubMed : 19432394, PubMed : 33031746). MUC2 networks form hydrogels that guard the underlying epithelium from pathogens and other hazardous matter entering from the outside world, while permitting nutrient absorption and gas exchange (PubMed : 33031746, PubMed : 36206754). Acts as a divalent copper chaperone that protects intestinal cells from copper toxicity and facilitates nutritional copper unptake into cells (PubMed : 36206754). Binds both Cu(2+) and its reduced form, Cu(1+), at two juxtaposed binding sites : Cu(2+), once reduced to Cu(1+) by vitamin C (ascorbate) or other dietary antioxidants, transits to the other binding site (PubMed : 36206754). MUC2-bound Cu(1+) is protected from oxidation in aerobic environments, and can be released for nutritional delivery to cells (PubMed : 36206754). Mucin gels store antimicrobial molecules that participate in innate immunity (PubMed : 33031746). Mucin glycoproteins also house and feed the microbiome, lubricate tissue surfaces, and may facilitate the removal of contaminants and waste products from the body (PubMed : 33031746). Goblet cells synthesize two forms of MUC2 mucin that differ in branched chain O-glycosylation and the site of production in the colon : a (1) 'thick' mucus that wraps the microbiota to form fecal pellets is produced in the proximal, ascending colon (By similarity). 'Thick' mucus transits along the descending colon and is lubricated by a (2) 'thin' MUC2 mucus produced in the distal colon which adheres to the 'thick' mucus (By similarity).
See full target information MUC2
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搭配使用产品

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Recombinant|SimpleStep

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  • 5
  • 4
Secondary Antibodies

AB150077

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
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Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

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Primary Antibodies

AB3649

Anti-Mucin 5AC antibody [45M1]

BOND RX™ Validated|Advanced Validation|Recombinant

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  • 5
  • 8

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