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Anti-MUC2抗体[B306.1] (ab7848)

Reviews (1)Q&A (1)References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [B306.1] (ab7848)

    Key features and details

    • Mouse monoclonal [B306.1] to MUC2
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

    概述

    • 产品名称

      Anti-MUC2抗体[B306.1]
      参阅全部 MUC2 一抗

    • 描述

      小鼠单克隆抗体[B306.1] to MUC2

    • 宿主

      Mouse

    • 特异性

      This antibody reacts with MUC2 gene product, a secretory apomucin found in a variety of normal and neoplastic human glandular epithelia.

    • 经测试应用

      适用于: IHC-Pmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Synthetic peptide corresponding to MUC2. MUC2 tandem repeat consensus sequence.

    • 阳性对照

      • Colon carcinoma

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab7848于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    1/4000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    说明
    IHC-P
    1/4000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    靶标

    • 功能

      Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.

    • 组织特异性

      Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.

    • 序列相似性

      Contains 1 CTCK (C-terminal cystine knot-like) domain.
      Contains 1 TIL (trypsin inhibitory-like) domain.
      Contains 2 VWFC domains.
      Contains 4 VWFD domains.

    • 翻译后修饰

      O-glycosylated.
      May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.
      At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway.

    • 细胞定位

      Secreted. In the intestine, secreted into the inner and outer mucus layers.
    • Information by UniProt

    • 数据库链接

      • 别名

        • Intestinal mucin 2 antibody
        • Intestinal mucin-2 antibody
        • MLP antibody
        • MUC 2 antibody
        • MUC-2 antibody
        • Muc2 antibody
        • MUC2_HUMAN antibody
        • Mucin 2 antibody
        • Mucin 2 intestinal antibody
        • Mucin 2 intestinal/tracheal antibody
        • Mucin 2 oligomeric mucus/gel forming antibody
        • Mucin 2 precursor antibody
        • Mucin 2 tracheal antibody
        • Mucin like protein antibody
        • Mucin-2 antibody
        • Mucin2 antibody
        • SMUC antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [B306.1] (ab7848)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [B306.1] (ab7848)
        Ab7848 staining human normal colon. Staining is localised to the membrane.
        Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
        Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

      文献 (1)

      发表研究结果有使用 ab7848？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab7848 被引用在 1 文献中.

      • Fishbein TM  et al. Rejection reversibly alters enteroendocrine cell renewal in the transplanted small intestine. Am J Transplant 9:1620-8 (2009). IHC-Fr ; Human . PubMed: 19519821

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      1-2 of 2 Abreviews or Q&A

      Western blot abreview for Anti-MUC2 antibody [B306.1]

       Poor
      Abreviews
      Application
      Western blot
      Sample
      Rat Tissue lysate - whole (GI)
      Loading amount
      50 µg
      Specification
      GI
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      proprietary as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Sep 27 2007

      Question

      I have inquiries about your products, ab7848 and ab8605. A researcher is looking for MUC1 antibody and MUC2 antibody. Please let me know if the ab7848 should detect MUC2 in gastric mucosa and the ab8605 should detect MUC1 in intestinal mucosa, respectively. I can't find these descriptions in your datasheets.

      Read More
      Answer

      Thank you for contacting us with your enquiry. The SwissProt database links on the datasheets for these two antibodies give some information on the tissue expression of Muc1 and muc2. Muc 2 (ab7848) is expressed in Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder. Muc 1 (ab8605) is expressed on ductal epithelial cells and on activated T-cells. Aberrantly glycosylated forms expressed in human epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. I have also searched a reference which gives details of Muc 1 (and Muc 2)expression in normal and tumour gastric and intestinal tissue: J Clin Pathol. 1996 July; 49(7): 560–564. Significance of MUC1 and MUC2 mucin expression in colorectal cancer. Y Ajioka et al. I hope this information is helpful. Should the customer have any further questions, please do not hesitate to contact us again.

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