重组Anti-MUC16抗体[EPSISR23] - BSA and Azide free (ab271903)
![Immunocytochemistry/ Immunofluorescence - Anti-MUC16 antibody [EPSISR23] - BSA and Azide free (ab271903)](https://www.abcam.cn/ps/products/271/ab271903/Images/ab271903-482304-anti-muc16-antibody-epsisr23-immunocytochemistry-nih-ovcar3-human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MUC16 antibody [EPSISR23] - BSA and Azide free (ab271903)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPSISR23] to MUC16 - BSA and Azide free
- Suitable for: ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-MUC16抗体[EPSISR23] - BSA and Azide free
参阅全部 MUC16 一抗 -
描述
兔单克隆抗体[EPSISR23] to MUC16 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details
不适用于: Flow Cyt (Intra),IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC/IF: NIH:OVCAR-3 cells;
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常规说明
ab271903 is the carrier-free version of ab134093.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPSISR23 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab271903于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
Can be blocked with ab139976 |
|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 2353 kDa.
|
| 说明 |
|---|
|
ICC/IF
Use at an assay dependent concentration. Can be blocked with ab139976 |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 2353 kDa. |
靶标
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功能
Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. -
组织特异性
Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas. -
序列相似性
Contains 2 ANK repeats.
Contains 56 SEA domains. -
结构域
Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail. -
翻译后修饰
Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts. -
细胞定位
Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain. - Information by UniProt
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数据库链接
- Entrez Gene: 94025 Human
- Omim: 606154 Human
- SwissProt: Q8WXI7 Human
- Unigene: 432676 Human
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别名
- CA 125 antibody
- CA-125 antibody
- CA125 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-MUC16 antibody [EPSISR23] - BSA and Azide free (ab271903)
This data was developed using ab134093, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells labeling MUC16 with Purified ab134093 at 1:50 dilution (2.3 µg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Anti-MUC16 antibody [EPSISR23] - BSA and Azide free (ab271903)](https://www.abcam.cn/ps/products/271/ab271903/Images/ab271903-3-benefits-of-recombinant-antibodies.png)
Anti-MUC16 antibody [EPSISR23] - BSA and Azide free (ab271903)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab271903 尚未被引用在任何文献中。
