重组Anti-MUC16抗体[EPSISR23] (ab134093)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPSISR23] to MUC16
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-MUC16抗体[EPSISR23]
参阅全部 MUC16 一抗 -
描述
兔单克隆抗体[EPSISR23] to MUC16 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details
不适用于: Flow Cyt (Intra),IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab139976) -
阳性对照
- ICC/IF: NIH:OVCAR-3 cells. WB: NIH:OVCAR-3 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPSISR23 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-MUC16 antibody [EPSISR23] - Low endotoxin, Azide free (ab215381)
- PE Anti-MUC16 antibody [EPSISR23] (ab215693)
- APC Anti-MUC16 antibody [EPSISR23] (ab221279)
- Anti-MUC16 antibody [EPSISR23] - BSA and Azide free (ab271903)
- Alexa Fluor® 488 Anti-MUC16 antibody [EPSISR23] (ab317979)
- Alexa Fluor® 594 Anti-MUC16 antibody [EPSISR23] (ab318020)
- Alexa Fluor® 555 Anti-MUC16 antibody [EPSISR23] (ab318062)
- Alexa Fluor® 647 Anti-MUC16 antibody [EPSISR23] (ab318104)
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134093于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/10000. Detects a band of approximately 2353 kDa (predicted molecular weight: 1519 kDa).Can be blocked with MUC16 peptide (ab139976).
For unpurified use at 1/10000 - 1/50000 dilution. |
|
ICC/IF |
1/50.
For unpurified use at 1/100 - 1/250 dilution. |
说明 |
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WB
1/10000. Detects a band of approximately 2353 kDa (predicted molecular weight: 1519 kDa).Can be blocked with MUC16 peptide (ab139976). For unpurified use at 1/10000 - 1/50000 dilution. |
ICC/IF
1/50. For unpurified use at 1/100 - 1/250 dilution. |
靶标
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功能
Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. -
组织特异性
Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas. -
序列相似性
Contains 2 ANK repeats.
Contains 56 SEA domains. -
结构域
Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail. -
翻译后修饰
Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts. -
细胞定位
Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain. - Information by UniProt
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数据库链接
- Entrez Gene: 94025 Human
- Omim: 606154 Human
- SwissProt: Q8WXI7 Human
- Unigene: 432676 Human
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别名
- CA 125 antibody
- CA-125 antibody
- CA125 antibody
see all
图片
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Immunocytochemistry analysis of NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) cells labeling MUC16 with Purified ab134093 at 1:50 dilution (2.3 µg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Anti-MUC16 antibody [EPSISR23] (ab134093) at 1/10000 dilution + NIH:OVCAR-3 (Human ovary adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 1519 kDa
Observed band size: 2353 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Image produced using the purified version.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab134093 被引用在 4 文献中.
- O'Callaghan AR et al. Human Oral Mucosal Fibroblasts from Limbal Stem Cell Deficient Patients as an Autologous Feeder Layer for Epithelial Cell Culture. Curr Eye Res 47:1106-1115 (2022). PubMed: 35577415
- O'Callaghan AR et al. Oral Mucosa Tissue Equivalents for the Treatment of Limbal Stem Cell Deficiency. Adv Biosyst 4:e1900265 (2020). PubMed: 32515079
- Fan Z et al. Smartphone Biosensor System with Multi-Testing Unit Based on Localized Surface Plasmon Resonance Integrated with Microfluidics Chip. Sensors (Basel) 20:N/A (2020). PubMed: 31941128
- O'Callaghan AR et al. Human-derived feeder fibroblasts for the culture of epithelial cells for clinical use. Regen Med 11:529-43 (2016). PubMed: 27513189