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Anti-mtTFA抗体- Mitochondrial Marker (ab47517)

Reviews (5)Q&A (4)References (22)
Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab47517)
  • Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody - Mitochondrial Marker (ab47517)
  • Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab47517)
  • Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab47517)

Key features and details

  • Rabbit polyclonal to mtTFA - Mitochondrial Marker
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-mtTFA antibody [RM1035] (ab307302)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-mtTFA抗体- Mitochondrial Marker
    参阅全部 mtTFA 一抗

  • 描述

    兔多克隆抗体to mtTFA - Mitochondrial Marker

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide corresponding to Human mtTFA aa 150 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab47516)

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab47517于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (4)
Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 29 kDa).
ICC/IF
Use a concentration of 1 µg/ml.
说明
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 29 kDa).
ICC/IF
Use a concentration of 1 µg/ml.

靶标

  • 功能

    Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M.

  • 序列相似性

    Contains 2 HMG box DNA-binding domains.

  • 细胞定位

    Mitochondrion.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • anscription factor 6-like 1 antibody
    • Mitochondrial transcription factor 1 antibody
    • mitochondrial transcription factor A antibody
    • MtTF1 antibody
    • mtTFA antibody
    • TCF 6 antibody
    • TCF-6 antibody
    • TCF6 antibody
    • TCF6L1 antibody
    • TCF6L2 antibody
    • TCF6L3 antibody
    • TFAM antibody
    • TFAM_HUMAN antibody
    • Transcription factor 6 antibody
    • Transcription factor 6 like 2 (mitochondrial transcription factor) antibody
    • Transcription factor 6 like 2 antibody
    • Transcription factor 6-like 2 antibody
    • transcription factor 6-like 3 antibody
    • Transcription factor A, mitochondrial antibody
    • Transcription factor A, mitochondrial antibody
    • Transcription factor A, mitochondrial precursor antibody
    see all

图片

  • Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab47517)
    Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab47517)
    All lanes : Anti-mtTFA antibody - Mitochondrial Marker (ab47517) at 1 µg/ml

    Lane 1 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Human heart tissue lysate - total protein (ab29431)
    Lane 3 : Brown adipose (Mouse) Tissue Lysate
    Lane 4 : Brown adipose (Rat) Tissue Lysate
    Lane 5 : Heart (Mouse) Tissue Lysate
    Lane 6 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 29 kDa
    Observed band size: 29 kDa
    Additional bands at: 100 kDa, 18 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab47517 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody - Mitochondrial Marker (ab47517)
    Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody - Mitochondrial Marker (ab47517)

    ICC/IF image of ab47517 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab47517, 1µg/ml) for 1h at room temperature. 1% BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab47517)
    Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab47517)
    All lanes : Anti-mtTFA antibody - Mitochondrial Marker (ab47517) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 29 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?
    Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.

  • Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab47517)
    Western blot - Anti-mtTFA antibody - Mitochondrial Marker (ab47517)
    All lanes : Anti-mtTFA antibody - Mitochondrial Marker (ab47517) at 2 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human mtTFA peptide (ab47516) at 2 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 29 kDa
    Observed band size: 24,26 kDa why is the actual band size different from the predicted?
    Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 10 minutes

文献 (22)

发表研究结果有使用 ab47517？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab47517 被引用在 22 文献中.

  • Capristo M  et al. Rapamycin rescues mitochondrial dysfunction in cells carrying the m.8344A > G mutation in the mitochondrial tRNALys. Mol Med 28:90 (2022). PubMed: 35922766
  • Tang Y  et al. Activation of PPARγ Protects Obese Mice from Acute Lung Injury by Inhibiting Endoplasmic Reticulum Stress and Promoting Mitochondrial Biogenesis. PPAR Res 2022:7888937 (2022). PubMed: 36213491
  • Zhang X  et al. Trib1 deficiency causes brown adipose respiratory chain depletion and mitochondrial disorder. Cell Death Dis 12:1098 (2021). PubMed: 34811364
  • Flax J  et al. OGA Inhibition Alters Energetics and Nutrient Sensing in Alzheimer's Disease Cytoplasmic Hybrids. J Alzheimers Dis 78:1743-1753 (2020). PubMed: 33285636
  • Bajzikova M  et al. Reactivation of Dihydroorotate Dehydrogenase-Driven Pyrimidine Biosynthesis Restores Tumor Growth of Respiration-Deficient Cancer Cells. Cell Metab 29:399-416.e10 (2019). PubMed: 30449682
View all Publications for this product

客户评价及客户问答

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1-9 of 9 Abreviews or Q&A

Western blot abreview for Anti-mtTFA antibody - Mitochondrial Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Guinea pig Tissue lysate - nuclear (heart)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-tris mini gel)
Loading amount
25 µg
Specification
heart
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 14 2021

Western blot abreview for Anti-mtTFA antibody - Mitochondrial Marker

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (human hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
human hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 19 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-mtTFA antibody - Mitochondrial Marker

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon and Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Specification
Colon and Kidney
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

提交于 Dec 13 2010

Western blot abreview for Anti-mtTFA antibody - Mitochondrial Marker

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing
Loading amount
12.5 µg
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jun 04 2010

Western blot abreview for Anti-mtTFA antibody - Mitochondrial Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293 cells)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris)
Loading amount
20 µg
Specification
HEK 293 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Sarah Ronnebaum

Verified customer

提交于 May 19 2009

Question

Thank you for your consideration and help.
After conducting the experiment to use ab47516 as blocking peptide, the specific bandwas therefore confirmed.
But the concernhecares aboutisthat thecontinued testing of ab47517costsboth their time andab47517 antibody.
Besides, the data of no primary control test showed no background to prove the problem was not from the secondary antibody.
Therefore, he would like to ask for a new vial of ab47517because mostab47517 antibody almostran outin testing. He is displeased about that.
Would you please help us deal with this issue?

Thanks and best regards,

Read More
Answer

Thank you for confirming the results of the customer. Unfortunately, Anja is not in the office at present and is therefore unable to help resolve this case but I will try my best in her place.

I think the results look much improved with the blocking peptide. Think continuing using the 5% BSA blocking and perhaps reducing the exposure time used should lead to further improvements. I can understand that your customer may be frustrated that he has been using up a lot of antibody to perform these optimisations and if he would like I can offer to send a free of charge vial of ab47517. If you would like for me to organise this please do let me know.

Read More

Question

LOT NUMBER GR30593-1 ORDER NUMBER xx DESCRIPTION OF THE PROBLEM Multiple bands (can’t distinguish which one was right) SAMPLE - Species: human - What’s cell line or tissue: primary fibroblast cell (from the newborns) - Cell extract or Nuclear extract: cell extract - Purified protein or Recombinant protein: Whole cell extraction PRIMARY ANTIBODY - Species: rabbit polyclonal - Reacts against: human - At what dilution(s) have you tested this antibody: 1:900 - What dilution buffer was used: 5% non-fat-milk in TBST - Incubation time: 1hr - Incubation temperature: room temperature - What washing steps were done: 15min x 1 + 5min x 3 DETECTION METHOD ECl+ ANTIBODY STORAGE CONDITIONS -20 SAMPLE PREPARATION - What lysis buffer was used: 20mM potassium phosphate buffer + sonication - What protease inhibitors were used: cocktail protease inhibitor - What loading buffer was used: NuPAGE LDS sample buffer (invitrogen:Cat no.NP0007) - Phosphatase inhibitors - Did you heat the samples: temperature and time: 70℃ for 10min AMOUNT OF PROTEIN LOADED 30ug ELECTROPHORESIS/GEL CONDITIONS - Reducing or non reducing gel: Reducing - Reducing agent: 2-ME - Gel percentage : 4-12% TRANSFER AND BLOCKING CONDITIONS - Transfer conditions: (Type of membrane, Protein transfer verified): PVDF, Coomassie blue (Invitrogen iBlot dry blotting system Cat no. IB10001) Blocking conditions - Buffer: 5% non-fat-milk in TBST - Blocking agent: milk, BSA, serum, what percentage: 5% - Incubation time: 1hr - Incubation temperature: room temperature SECONDARY ANTIBODY - Species: goat - Reacts against: rabbit - At what dilution(s) have you tested this antibody: 1:5000 - Incubation time: 1hr - Wash steps: 15min x 1 + 5min x 3 - Fluorochrome or enzyme conjugate: enzyme conjugate - Do you know whether the problems you are experiencing come from the secondary? HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 1 HAVE YOU RUN A "NO PRIMARY" CONTROL? No

Read More
Answer

Thank you for contacting us.

I am sorry to hear you have been experiencing problems with a vial of anti-mtTFA antibody ab47517.

We have received no complaints about this product therefore I am concerned it is not working for you. I appreciate the time taken to submit further information to us and I would like to make some suggestions to the protocol in an attempt to improve the results obtained:

When testing this antibody, our lab used 5% BSA as a blocking reagent, so I recommend switching to this instead of milk. Some antibodies give stronger, more specific signals on blots blocked with BSA instead of milk, so doing this may improve the results you are seeing, and reduce the non-specific bands between 25 and 34 kDa. An example of the above (BSA instead of milk)is the western blot obtained with the Abcam GAPDH antibody ab9385 (https://www.abcam.com/HRP-GAPDH-antibody-Loading-Control-ab9385.html (or use the following: https://www.abcam.com/HRP-GAPDH-antibody-Loading-Control-ab9385.html).)

I hope this suggestion will improve the results. As the antibody was purchased inDecember you are covered by our Abpromise guarantee. Therefore if the suggestion does not improve the results I would be happy to offer a refund or replacement vial. Please do not hesitate to contact me should you have additional questions.

Read More

Question

Hi, The power cut was not long than 24 hours and we cannot assure if the products were or not at room temperature when the electricity switched again (they were kept in the fridge but it was not working as a consequence of the power cut). We need your evaluation about the state of the products, because if they are damaged we will claim it cost to the insurance company and we will process with its reposition. Thanks in advance for your help and collaboration Kind regards,    

Read More
Answer

Thank you for contacting us. My colleague "Ariana" has already sent her reply so I am closing this case. The antibodies are fine for 1 week at room temperature however in your case if the products fail to perform I would suggest getting refund from insurance company. You can get information of product price from our website at  https://www.abcam.com/ I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.    

Read More

Question

Dear Sir/Madame, Recently IRB Barcelona suffered a power cut and it caused that refrigerators and freezers located in our plant were out of operating. In order to proceed with an official claim for the possible costs that could arise from damaged products as a result of their inadequate storage, we will appreciate your evaluation about the items or products that should be rejected and destroyed because their proper running could not being guaranteed. Please find below the items/products affected Supplier Ref. ITEM Description Abcam ab25631 Ms mAb to LAMP2[H4B4] Abcam ab47517 mtTFA antibody (ab47517 Abcam ab56889 Anticòs monoclonal Mfn2     Thanks in advance for your help and cooperation.   Kind regards.      

Read More
Answer

Thank you for contacting us. In order to help you with your question could you provide the following - Order number of purchase - for how long the power cut happened? -Were these products at room temperature when the electricity switched on again? What do you really want us to do? Do you need quote for these products please explain? I look forward to hearing from you soon.

Read More

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