Anti-mtTFA抗体(ab272885)
Key features and details
- Rabbit polyclonal to mtTFA
- Suitable for: WB, ICC/IF, IHC-P, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-mtTFA抗体
参阅全部 mtTFA 一抗 -
描述
兔多克隆抗体to mtTFA -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-P, IPmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant full length protein corresponding to Human mtTFA.
Database link: Q00059 -
阳性对照
- WB: HEK-293T, A431, HeLa, HepG2, Neuro-2a and PC-12 whole cell lysate. IHC-P: Human breast cancer tissue. ICC/IF: HeLa and NTERA-2 cl.D1 [NT2/D1] cells. IP: HEK-293T whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272885于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000.
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| ICC/IF |
1/100 - 1/1000.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IP |
1/100 - 1/500.
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| 说明 |
|---|
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WB
1/500 - 1/3000. |
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ICC/IF
1/100 - 1/1000. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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IP
1/100 - 1/500. |
靶标
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功能
Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. target DNA. Interacts with TFB1M and TFB2M. -
序列相似性
Contains 2 HMG box DNA-binding domains. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 7019 Human
- Entrez Gene: 21780 Mouse
- Entrez Gene: 83474 Rat
- Omim: 600438 Human
- SwissProt: Q00059 Human
- SwissProt: P40630 Mouse
- SwissProt: Q91ZW1 Rat
- Unigene: 642966 Human
see all -
别名
- anscription factor 6-like 1 antibody
- Mitochondrial transcription factor 1 antibody
- mitochondrial transcription factor A antibody
see all
图片
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Western blot - Anti-mtTFA antibody (ab272885)All lanes : Anti-mtTFA antibody (ab272885) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.12% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody (ab272885)Paraffin-embedded human breast cancer tissue stained for mtTFA using ab272885 at 1/250 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
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Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody (ab272885)4% PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for mtTFA using ab272885 at 1/500 dilution in ICC/IF. Red: Mitotracker, a mitochondria marker, diluted at 1:2000. Blue: Hoechst 33342 staining.
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Immunoprecipitation - Anti-mtTFA antibody (ab272885)Immunoprecipitation - Anti-mtTFA antibody (ab272885).
Sample: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
A. 40 μg HEK-293T whole cell lysate.
B. Control with 2 μg of preimmune rabbit IgG
C. Immunoprecipitation of mtTFA protein by 2 μg of ab272885
12% SDS-PAGE
The immunoprecipitated mtTFA protein was detected by ab272885 diluted at 1:1000, followed by anti-rabbit IgG-HRP secondary antibody.
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Western blot - Anti-mtTFA antibody (ab272885)Anti-mtTFA antibody (ab272885) at 1/1000 dilution + Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 30 µg12% SDS-PAGE
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Western blot - Anti-mtTFA antibody (ab272885)All lanes : Anti-mtTFA antibody (ab272885) at 1/500 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 2 : Rat2 (rat fibroblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody12% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody (ab272885)2% paraformaldehyde/culture medium fixed NTERA-2 cl.D1 [NT2/D1] (Human malignant pluripotent embryonic carcinoma cell line) cells stained for mtTFA using ab272885 at 1/500 dilution in ICC/IF. Blue: Hoechst 33342 staining.
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Western blot - Anti-mtTFA antibody (ab272885)All lanes : Anti-mtTFA antibody (ab272885) at 1/3000 dilution
Lane 1 : Non-transfected HEK-293T whole cell extracts
Lane 2 : mTFA shRNA transfected HEK-293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG12% SDS-PAGE
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab272885 被引用在 5 文献中.
- Um JH et al. Selective induction of Rab9-dependent alternative mitophagy using a synthetic derivative of isoquinoline alleviates mitochondrial dysfunction and cognitive deficits in Alzheimer's disease models. Theranostics 14:56-74 (2024). PubMed: 38164158
- Xia Q et al. Peroxiredoxin 2 is required for the redox mediated adaptation to exercise. Redox Biol 60:102631 (2023). PubMed: 36791646
- Luo XM et al. The purine metabolite inosine monophosphate accelerates myelopoiesis and acute pancreatitis progression. Commun Biol 5:1088 (2022). PubMed: 36224248
- Wu J et al. Activation of AMPK-PGC-1α pathway ameliorates peritoneal dialysis related peritoneal fibrosis in mice by enhancing mitochondrial biogenesis. Ren Fail 44:1545-1557 (2022). PubMed: 36148521
- Ren Y et al. Milk fat globule EGF factor 8 restores mitochondrial function via integrin-medicated activation of the FAK-STAT3 signaling pathway in acute pancreatitis. Clin Transl Med 11:e295 (2021). PubMed: 33634976