Anti-MTR4抗体

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTR4 antibody (AB70551)

IHC image of ab70551 staining in human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70551, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTR4 antibody (AB70551)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling MTR4 with ab70551 at 1/200 (1μg/ml). Detection : DAB.

• IP

Supplier Data

Immunoprecipitation - Anti-MTR4 antibody (AB70551)

Detection of human MTR4 by western blot of immunoprecipitates. Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies : Affinity purified rabbit anti-MTR4 antibody ab70551 used for IP at 3 μg per reaction. MTR4 was also immunoprecipitated by rabbit anti-MTR4 antibody ab70552. For blotting immunoprecipitated MTR4, ab70551 was used at 1 μg/ml. Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-MTR4 antibody (ab70551)

Predicted band size: 118 kDa

false

• WB

Supplier Data

Western blot - Anti-MTR4 antibody (AB70551)

Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Western blot - Anti-MTR4 antibody (ab70551) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Predicted band size: 118 kDa

false