Name: Anti-mTOR (phospho S2448) antibody [EPR426(2)]
Brand: RabMAb
SKU: ab109268
Rating: 4 (6 reviews)

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Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR426(2)] to mTOR (phospho S2448)
Suitable for: WB, IHC-P, Dot blot, IHC-Fr
Reacts with: Mouse, Human

Carrier Free

产品名称

Anti-mTOR (phospho S2448)抗体[EPR426(2)]
参阅全部 mTOR 一抗
描述

兔单克隆抗体[EPR426(2)] to mTOR (phospho S2448)
宿主

Rabbit
经测试应用

适用于: WB, IHC-P, Dot blot, IHC-Frmore details
不适用于: Flow Cyt,ICC/IF or IP
种属反应性

与反应: Mouse, Human
预测可用于: Rat, Pig
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: HEK293, HeLa and NIH/3T3 cell lysates. IHC-P: Human breast and endometrium carcinoma tissue. Dot Blot: mTOR (phospho S2448) phospho peptide. IHC-Fr: Human heart tissue sections.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
存储溶液

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR426(2)
同种型

IgG
研究领域
- Neuroscience
- Development
- Neuroscience
- Processes

Alternative Versions
- Anti-mTOR (phospho S2448) antibody [EPR426(2)] - BSA and Azide free (ab177734)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human mTOR protein (ab114179)

The Abpromise guarantee

Abpromise™承诺保证使用ab109268于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB	(4)	1/1000 - 1/10000. Predicted molecular weight: 289 kDa.
IHC-P	(1)	1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Dot blot		1/1000.
IHC-Fr		Use at an assay dependent concentration.

说明
WB 1/1000 - 1/10000. Predicted molecular weight: 289 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Dot blot 1/1000.
IHC-Fr Use at an assay dependent concentration.

应用说明

Is unsuitable for Flow Cyt,ICC/IF or IP.

功能

Kinase subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Phosphorylates MAF1 leading to attenuation of its RNA polymerase III-repressive function. mTORC2 is also activated by growth. factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
组织特异性

Expressed in numerous tissues, with highest levels in testis.
序列相似性

Belongs to the PI3/PI4-kinase family.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 7 HEAT repeats.
Contains 1 PI3K/PI4K domain.
翻译后修饰

Autophosphorylated; when part of mTORC1 or mTORC2.
细胞定位

Endoplasmic reticulum membrane. Golgi apparatus membrane. Mitochondrion outer membrane. Lysosome. Cytoplasm. Nucleus > PML body. Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB.
Target information above from: UniProt accession P42345 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 2475 Human
- Entrez Gene: 56717 Mouse
- Entrez Gene: 56718 Rat
- Omim: 601231 Human
- SwissProt: P42345 Human
- SwissProt: Q9JLN9 Mouse
- SwissProt: P42346 Rat
- Unigene: 338207 Human
- Unigene: 21158 Mouse
- Unigene: 11008 Rat
see all
别名
- dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibody
- FK506 binding protein 12 rapamycin associated protein 1 antibody
- FK506 binding protein 12 rapamycin associated protein 2 antibody
- FK506 binding protein 12 rapamycin complex associated protein 1 antibody
- FK506-binding protein 12-rapamycin complex-associated protein 1 antibody
- FKBP rapamycin associated protein antibody
- FKBP12 rapamycin complex associated protein antibody
- FKBP12-rapamycin complex-associated protein 1 antibody
- FKBP12-rapamycin complex-associated protein antibody
- FLJ44809 antibody
- FRAP antibody
- FRAP1 antibody
- FRAP2 antibody
- Mammalian target of rapamycin antibody
- Mechanistic target of rapamycin antibody
- mTOR antibody
- MTOR_HUMAN antibody
- OTTHUMP00000001983 antibody
- RAFT1 antibody
- Rapamycin and FKBP12 target 1 antibody
- Rapamycin associated protein FRAP2 antibody
- Rapamycin target protein 1 antibody
- Rapamycin target protein antibody
- RAPT1 antibody
- Serine/threonine-protein kinase mTOR antibody
see all

Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)

All lanes : Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) at 1/2000 dilution (Purified)

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa grown in serum-free media overnight, then treated with 200nM PMA for 4 hours whole cell lysate
Lane 3 : HeLa grown in serum-free media overnight, then treated with 200nM PMA for 4 hours whole cell lysate. Then the membrane was incubated with alkaline phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 289 kDa
Observed band size: 289 kDa

Blocking buffer: 5% NFDM/TBST
Dilutiing buffer: 5% NFDM /TBST
Immunohistochemistry (Frozen sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)

IHC image of mTOR (phospho S2448) staining in a section of frozen normal human heart performed on a Leica BOND^TM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab109268, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using unpurified ab109268 at a dilution of 1/50. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Dot Blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)

Dot blot analysis of mTOR (phospho S2448) phospho peptide (Lane 1) and mTOR non-phospho peptide (Lane 2) labeling mTOR (phospho S2448) phospho peptide with purified ab109268 at a dilution of 1/1000 (0.073ug/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/100,000.

Blocking buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM /TBST
Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)

All lanes : Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) at 1/1000 dilution (purified)

Lane 1 : untreated NIH/3T3 cell lysate
Lane 2 : NIH/3T3 cell lysate treated with insulin
Lane 3 : NIH/3T3 cell lysate treated with insulin, then the membrane treated wth alkaline phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (HRP goat anti-rabbit IgG (H+L))

Predicted band size: 289 kDa
Observed band size: 289 kDa

Exposure time: 2 minutes

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)

Immunohistochemical cytoplasmic and nuclear staining of paraffin embedded human endometrium carcinoma with purified ab109268 at a working dilution of 1 in 100. The secondary antibody used is ab97051, a HRP goat anti-rabbit IgG (H+L), at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)

All lanes : Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) at 1/1000 dilution (purified)

Lane 1 : untreated HeLa cell lysate
Lane 2 : HeLa cell lysate treated with insulin
Lane 3 : HeLa cell lystae treated with insulin, and the membrane treated with alkaline phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/2000 dilution (HRP goat anti-rabbit (H+L))

Predicted band size: 289 kDa
Observed band size: 289 kDa

Exposure time: 1 minute

Blocking buffer: 2% BSA/TBST
Dilution buffer: 2% BSA/TBST
Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)

All lanes : Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) at 1/2000 dilution (purified)

Lane 1 : untreated HEK293 cell lysate
Lane 2 : HEK293 cell lysate treated with alkaline phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (HRP goat anti-rabbit IgG (H+L))

Predicted band size: 289 kDa
Observed band size: 289 kDa

Exposure time: 3 minutes

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)

All lanes : Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) at 1/5000 dilution (unpurified)

Lane 1 : untreated HEK293 cell lysate
Lane 2 : HEK293 cell lysate treated with alkaline phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/2000 dilution (HRP goat anti-rabbit IgG (H+L))

Predicted band size: 289 kDa
Observed band size: 289 kDa

Exposure time: 3 minutes

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

发表研究结果有使用 ab109268？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab109268 被引用在 519 文献中.

Shi S et al. FGF19 promotes nasopharyngeal carcinoma progression by inducing angiogenesis via inhibiting TRIM21-mediated ANXA2 ubiquitination. Cell Oncol (Dordr) 47:283-301 (2024). PubMed: 37782406
Lu Y et al. Impairment of Autophagy Mediates the Uric-Acid-Induced Phenotypic Transformation of Vascular Smooth Muscle Cells. Pharmacology 109:34-42 (2024). PubMed: 38011839
Liu C et al. High-Glucose-Induced Injury to Proximal Tubules of the Renal System Is Alleviated by Netrin-1 Suppression of Akt/mTOR. J Diabetes Res 2023:4193309 (2023). PubMed: 38033740
Shen L et al. Integrated transcriptomics, proteomics, and functional analysis to characterize the tissue-specific small extracellular vesicle network of breast cancer. MedComm (2020) 4:e433 (2023). PubMed: 38053815
Jiang F et al. The transcription factor TBP promotes hepatocellular carcinoma progression by activating AKT3. Am J Cancer Res 13:5656-5666 (2023). PubMed: 38058816

View all Publications for this product

客户评价及客户问答

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1-4 of 4 Abreviews or Q&A

Application

Western blot

Sample

Rat Cell lysate - whole cell (PC12 cells)

Gel Running Conditions

Reduced Denaturing

Loading amount

20 µg

Treatment

5 uM of alpha-7 nAChR modulator for 6, 24, 48 hours

Specification

PC12 cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 05 2023

Application

Western blot

Sample

Mouse Cell lysate - whole cell (primary cultured mouse cortical neurons)

Gel Running Conditions

Reduced Denaturing

Loading amount

200000 cells

Specification

primary cultured mouse cortical neurons

Blocking step

BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 02 2020

Application

Western blot

Sample

Human Cell lysate - whole cell (C42B Prostate cancer cell line)

Gel Running Conditions

Reduced Denaturing

Loading amount

20 µg

Specification

C42B Prostate cancer cell line

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Dimitra Kalamida

Verified customer

提交于 Dec 25 2019

Application

Western blot

Loading amount

20 µg

Gel Running Conditions

Reduced Denaturing (4-12)

Sample

Mouse Cell lysate - whole cell (placenta)

Specification

placenta

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Varkha Agrawal

Verified customer

提交于 Jul 14 2014

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Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

组织特异性

序列相似性

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (519)

客户评价及客户问答

Western blot abreview for Anti-mTOR (phospho S2448) antibody [EPR426(2)]

Western blot abreview for Anti-mTOR (phospho S2448) antibody [EPR426(2)]

Western blot abreview for Anti-mTOR (phospho S2448) antibody [EPR426(2)]

Western blot abreview for Anti-mTOR (phospho S2448) [EPR426(2)] antibody