Anti-mTOR (phospho S2448)抗体(ab131538)
Key features and details
- Rabbit polyclonal to mTOR (phospho S2448)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-mTOR (phospho S2448)抗体
参阅全部 mTOR 一抗 -
描述
兔多克隆抗体to mTOR (phospho S2448) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human mTOR (phospho S2448) conjugated to keyhole limpet haemocyanin.
Database link: NP_004949.1 -
阳性对照
- MCF cells; 293 cell extract untreated or PMA-treated; Human breast carcinoma tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.88% Sodium chloride, 49% PBS, 50% Glycerol (glycerin, glycerine)
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab131538 was purified by affinity chromatography using epitope-specific phosphopeptide. Non-phosphospecific antibodies were removed by chromatography using non-phosphopeptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131538于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 289 kDa.
|
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| IHC-P |
1/50 - 1/100.
|
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| ICC/IF | (1) |
1/100 - 1/200.
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 289 kDa. |
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IHC-P
1/50 - 1/100. |
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ICC/IF
1/100 - 1/200. |
靶标
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功能
Kinase subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Phosphorylates MAF1 leading to attenuation of its RNA polymerase III-repressive function. mTORC2 is also activated by growth. factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. -
组织特异性
Expressed in numerous tissues, with highest levels in testis. -
序列相似性
Belongs to the PI3/PI4-kinase family.
Contains 1 FAT domain.
Contains 1 FATC domain.
Contains 7 HEAT repeats.
Contains 1 PI3K/PI4K domain. -
翻译后修饰
Autophosphorylated; when part of mTORC1 or mTORC2. -
细胞定位
Endoplasmic reticulum membrane. Golgi apparatus membrane. Mitochondrion outer membrane. Lysosome. Cytoplasm. Nucleus > PML body. Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB. - Information by UniProt
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数据库链接
- Entrez Gene: 2475 Human
- Omim: 601231 Human
- SwissProt: P42345 Human
- Unigene: 338207 Human
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别名
- dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibody
- FK506 binding protein 12 rapamycin associated protein 1 antibody
- FK506 binding protein 12 rapamycin associated protein 2 antibody
see all
图片
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Western blot - Anti-mTOR (phospho S2448) antibody (ab131538)All lanes : Anti-mTOR (phospho S2448) antibody (ab131538) at 1/500 dilution
Lane 1 : 293 cell extract (untreated)
Lane 2 : 293 cell extract (PMA-treated)
Predicted band size: 289 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-mTOR (phospho S2448) antibody (ab131538)Immunofluorescence analysis of methanol-fixed MCF cells labelling mTOR (phospho S2448) with ab131538 at 1/100 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody (ab131538)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling mTOR (phospho S2448) with ab131538 at 1/50 dilution. Right panel was preincubated with blocking peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (21)
ab131538 被引用在 21 文献中.
- Zhu S et al. DNA methylation and miR-92a-3p-mediated repression of HIP1R promotes pancreatic cancer progression by activating the PI3K/AKT pathway. J Cell Mol Med 27:788-802 (2023). PubMed: 36811277
- Li X et al. Suppression of FAM83D Inhibits Glioma Proliferation, Invasion and Migration by Regulating the AKT/mTOR Signaling Pathway. Transl Oncol 22:101454 (2022). PubMed: 35617811
- Zhan CH et al. The cancer-testis antigen a-kinase anchor protein 3 facilitates breast cancer progression via activation of the PTEN/PI3K/AKT/mTOR signaling. Bioengineered 13:8478-8489 (2022). PubMed: 35322748
- Zhao L et al. mTORC1-c-Myc pathway rewires methionine metabolism for HCC progression through suppressing SIRT4 mediated ADP ribosylation of MAT2A. Cell Biosci 12:183 (2022). PubMed: 36371321
- Tang Z et al. Sestrin2-Mediated Autophagy Contributes to Drug Resistance via Endoplasmic Reticulum Stress in Human Osteosarcoma. Front Cell Dev Biol 9:722960 (2021). PubMed: 34646824