Anti-MTIF2抗体(ab121243)
Key features and details
- Rabbit polyclonal to MTIF2
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MTIF2抗体
参阅全部 MTIF2 一抗 -
描述
兔多克隆抗体to MTIF2 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human MTIF2 aa 497-646.
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阳性对照
- Human cell line A431, Human plasma lysate, Human nasopharynx tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121243于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| WB | (1) |
Use a concentration of 0.04 - 0.4 µg/ml.
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| 说明 |
|---|
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IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
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功能
One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex. -
组织特异性
Expressed in all tissues examined. Highest level in skeletal muscle. -
序列相似性
Belongs to the IF-2 family. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 4528 Human
- Omim: 603766 Human
- SwissProt: P46199 Human
- Unigene: 149894 Human
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别名
- IF-2(Mt) antibody
- IF-2Mt antibody
- IF2(mt) antibody
see all
图片
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Western blot - Anti-MTIF2 antibody (ab121243)All lanes : Anti-MTIF2 antibody (ab121243) at 1/250 dilution
Lane 1 : Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2 : RT-4 cell lysate
Lane 3 : U-251 MG cell lysate
Lane 4 : Human Plasma lysate
Lane 5 : Human liver lysate
Lane 6 : Human tonsil lysate -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTIF2 antibody (ab121243)Immunohistochemical staining of paraffin embedded Human nasopharynx tissue, using 1/25 ab121243, shows strong membranous positivity in respiratory epithelial cells. -
Immunocytochemistry/ Immunofluorescence - Anti-MTIF2 antibody (ab121243)Immunofluorescent staining, using 4 µg/ml ab121243, in Human cell line A431 shows positivity in mitochondria. Antibody staining is shown in green.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab121243 尚未被引用在任何文献中。