重组Anti-MTHFD2抗体[EPR26938-20]
Anti-MTHFD2 antibody [EPR26938-20]
- BOND RX™ Validated
- 20ul selling size
- RabMAb
- Recombinant
- KO Validated
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(2 Publications)
Rabbit Recombinant Monoclonal MTHFD2 antibody. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with Mouse, Human, Rat samples. Cited in 2 publications.
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NMDMC, MTHFD2
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-MTHFD2 antibody [EPR26938-20] (AB307428)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Wild-type 293T (human embryonic kidney epithelial cell, Right) / MTHFD2 knockout 293T (Left) cells labelling MTHFD2 with ab307428 at 1/5000 dilution (0.01ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Positive staining on 293T cells (ab255449), while no staining on MTHFD2 knockout 293T cells (ab266383).
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD2 antibody [EPR26938-20] (AB307428)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue lABeling MTHFD2 with ab307428 at 1/500 (1.008 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human tonsil (PMID : 24451681). The section was incubated with ab307428 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD2 antibody [EPR26938-20] (AB307428)
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue lABeling MTHFD2 with ab307428 at 1/500 (1.008 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human breast cancer (PMID : 26461067). The section was incubated with ab307428 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-MTHFD2 antibody [EPR26938-20] (AB307428)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MTHFD2 KO HEK293T (MTHFD2 knockout human embryonic kidney epithelial cell), ab266383 cells labelling MTHFD2 with ab307428 at 1/1000 (0.504 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing mitochondrial staining in wildtype HEK293T cells and no staining in MTHFD2 knockout HEK293T cells. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. ab197491 Anti-COX IV Mouse monoclonal antibody (Alexa Fluor® 647) - Mitochondrial Marker was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD2 antibody [EPR26938-20] (AB307428)
Immunohistochemical analysis of paraffin-embedded (A) Wild-type HEK293 tissue labeling MTHFD2 with ab307428 at 1/500 (1.008 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on (A) Wild-type HEK293T (human embryonic kidney epithelial cell) cell pellet, no staining on (B) MTHFD2 knockout HEK293T (ab266383) cell pellet. The section was incubated with ab307428 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- IP
Supplier Data
Immunoprecipitation - Anti-MTHFD2 antibody [EPR26938-20] (AB307428)
MTHFD2 was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 10 ug with ab307428 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307428 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 10 ug
Lane 2 : ab307428 IP in HEK-293 whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307428 in HEK-293 whole cell lysate
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 1 second
All lanes:
Immunoprecipitation - Anti-MTHFD2 antibody [EPR26938-20] (ab307428) at 1/1000 dilution
Lane 1:
HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 10 μg
Lane 2:
HEK-293 whole cell lysate
Secondary
All lanes:
Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution
Observed band size: 35 kDa
false
Exposure time: 1s
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD2 antibody [EPR26938-20] (AB307428)
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue lABeling MTHFD2 with ab307428 at 1/500 (1.008 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on rat spleen.The section was incubated with ab307428 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MTHFD2 antibody [EPR26938-20] (AB307428)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue lABeling MTHFD2 with ab307428 at 1/500 (1.008 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse spleen.The section was incubated with ab307428 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-MTHFD2 antibody [EPR26938-20] (AB307428)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MEF (mouse embryo fibroblast) cells labelling MTHFD2 with ab307428 at 1/1000 (0.504 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in MEF cell line. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-MTHFD2 antibody [EPR26938-20] (AB307428)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MEF (mouse embryonic fibroblast (immortalized)) cells labelling MTHFD2 with ab307428 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
- IP
Supplier Data
Immunoprecipitation - Anti-MTHFD2 antibody [EPR26938-20] (AB307428)
MTHFD2 was immunoprecipitated from 0.35 mg MEF (mouse embryonic fibroblast) whole cell lysate 10 ug with ab307428 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307428 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1 : MEF (mouse embryonic fibroblast) whole cell lysate 10 ug
Lane 2 : ab307428 IP in MEF whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307428 in MEF whole cell lysate
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 10 seconds
All lanes:
Immunoprecipitation - Anti-MTHFD2 antibody [EPR26938-20] (ab307428) at 1/1000 dilution
Lane 1:
MEF (mouse embryonic fibroblast) whole cell lysate 10 μg
Lane 2:
MEF whole cell lysate
Secondary
All lanes:
Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution
Observed band size: 35 kDa
false
Exposure time: 10s
- WB
Supplier Data
Western blot - Anti-MTHFD2 antibody [EPR26938-20] (AB307428)
Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : Lane 1 : 15 seconds, Lanes 2-6 and 8-9 : 180 seconds, Lane 7 : 48 seconds.
All lanes:
Western blot - Anti-MTHFD2 antibody [EPR26938-20] (ab307428) at 1/1000 dilution
Lane 1:
786-O (Human kidney renal cell adenocarcinoma) whole cell lysate 20 μg
Lane 2:
LNCaP (human prostate carcinoma epithelial cell) whole cell lysate 20 μg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 35 kDa
false
Exposure time: 48s
- WB
Supplier Data
Western blot - Anti-MTHFD2 antibody [EPR26938-20] (AB307428)
Blocking and diluting buffer and concentration : 5% NFDM/TBSTIn Western blot, ab307428 was shown to bind specifically to MTHFD2. A band was observed at 35 kDa in wild-type HEK-293 cell lysates whereas no signal observed at this size in MTHFD2 knockout cell line ab266383 (knockout cell lysate ab258528). Exposure time : 15 seconds
All lanes:
Western blot - Anti-MTHFD2 antibody [EPR26938-20] (ab307428) at 1/1000 dilution
Lane 1:
Wild-type HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 20 μg
Lane 2:
MTHFD2 knockout HEK-293 whole cell lysate 20 μg
Lane 3:
MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate 20 μg
Lane 4:
MEF (mouse embryonic fibroblast) whole cell lysate 20 μg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 35 kDa
false
Exposure time: 15s
- WB
Supplier Data
Western blot - Anti-MTHFD2 antibody [EPR26938-20] (AB307428)
Blocking and diluting buffer and concentration : 5% NFDM/TBSTThe Lanes 2-6 were developed using a high sensitivity ECL substrate. Exposure time :
All lanes:
Western blot - Anti-MTHFD2 antibody [EPR26938-20] (ab307428) at 1/1000 dilution
Lane 1:
Human tonsil tissue lysate 20 μg
Lane 2:
Human colon tissue lysate 20 μg
Lane 3:
Human colon cancer tissue lysate 20 μg
Lane 4:
Human testis tissue lysate 20 μg
Lane 5:
Human ovary tissue lysate 20 μg
Lane 6:
Mouse breast cancer tissue lysate 20 μg
Lane 7:
Mouse pancreas tissue lysate 20 μg
Lane 8:
Mouse kidney tissue lysate 20 μg
Lane 9:
Rat kidney tissue lysate 20 μg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 35 kDa
true
Exposure time: 15s
不同偶联物与剂型 (3)
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Anti-MTHFD2 antibody [EPR26938-20] - BSA and Azide free
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519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-MTHFD2 antibody [EPR26938-20]
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665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-MTHFD2 antibody [EPR26938-20]
反应性数据
产品详情
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
性能和储存信息
形式
纯化工艺
存储溶液
运输条件
推荐的短期储存时间
推荐的短期储存条件
推荐的长期储存条件
分装信息
储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Methylenetetrahydrofolate dehydrogenase plays an important role in folate metabolism contributing to the one-carbon metabolic pathway. MTHFD2 is part of the tetrahydrofolate (THF) complex critical for DNA synthesis and repair. The enzyme supports the production of nucleotides and amino acids by providing necessary one-carbon units linking it to rapid cellular proliferation as seen in cancerous cells.
Pathways
MTHFD2 is key in connecting the folate-mediated one-carbon metabolic network to the central metabolism. It is involved in the metabolic pathways that synthesize purines and thymidylate. In these networks MTHFD2 interacts with proteins such as SHMT2 (serine hydroxymethyltransferase 2) facilitating serine to glycine conversion which is important for the provision of one-carbon donors for methylation reactions.
产品实验方案
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靶点信息
文献 (2)
Recent publications for all applications. Explore the full list and refine your search
International journal of biological sciences 20:4277-4296 PubMed39247810
2024
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Renal failure 46:2369342 PubMed39230047
2024
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