Anti-MTHFD1抗体(ab70203)
Key features and details
- Mouse polyclonal to MTHFD1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MTHFD1抗体
参阅全部 MTHFD1 一抗 -
描述
小鼠多克隆抗体to MTHFD1 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human MTHFD1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- MTHFD1 transfected 293T cell lysate, human placenta cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70203于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 102 kDa).
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 102 kDa). |
靶标
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组织特异性
Ubiquitous. -
通路
One-carbon metabolism; tetrahydrofolate interconversion. -
疾病相关
Defects in MTHFD1 may be a cause of susceptibility to folate-sensitive neural tube defects (folate-sensitive NTD) [MIM:601634]. The most common NTDs are open spina bifida (myelomeningocele) and anencephaly. Genetic defects in MTHFD1 may affect the risk of spina bifida via the maternal rather than the embryonic genotype.
Genetic variation in MTHFD1 could be associated with susceptibility to colorectal cancer (CRC) [MIM:114500]. -
序列相似性
In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family. -
结构域
This trifunctional enzyme consists of two major domains: an N-terminal part containing the methylene-THF dehydrogenase and cyclohydrolase activities and a larger C-terminal part containing formyl-THF synthetase activity. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 4522 Human
- Omim: 172460 Human
- SwissProt: P11586 Human
- Unigene: 652308 Human
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别名
- 5,10 methylenetetrahydrofolate dehydrogenase 5,10 methylenetetrahydrofolate cyclohydrolase,10 formyltetrahydrofolate synthetase antibody
- C 1 tetrahydrofolate synthase cytoplasmic antibody
- C1 tetrahydrofolate synthase cytoplasmic antibody
see all
图片
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Western blot - Anti-MTHFD1 antibody (ab70203)Anti-MTHFD1 antibody (ab70203) at 1/500 dilution + human placenta at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 102 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted? -
Western blot - Anti-MTHFD1 antibody (ab70203)All lanes : Anti-MTHFD1 antibody (ab70203) at 1/500 dilution
Lane 1 : MTHFD1 transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 102 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
数据表及文件
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Datasheet download
文献 (6)
ab70203 被引用在 6 文献中.
- Tanaka K et al. Glioma cells require one-carbon metabolism to survive glutamine starvation. Acta Neuropathol Commun 9:16 (2021). PubMed: 33468252
- Di Marcantonio D et al. ATF3 coordinates serine and nucleotide metabolism to drive cell cycle progression in acute myeloid leukemia. Mol Cell 81:2752-2764.e6 (2021). PubMed: 34081901
- Sdelci S et al. MTHFD1 interaction with BRD4 links folate metabolism to transcriptional regulation. Nat Genet 51:990-998 (2019). PubMed: 31133746
- Yu H et al. Overexpression of MTHFD1 in hepatocellular carcinoma predicts poorer survival and recurrence. Future Oncol 15:1771-1780 (2019). PubMed: 30997850
- Zheng Y et al. Mitochondrial One-Carbon Pathway Supports Cytosolic Folate Integrity in Cancer Cells. Cell 175:1546-1560.e17 (2018). PubMed: 30500537
- Ducker GS et al. Human SHMT inhibitors reveal defective glycine import as a targetable metabolic vulnerability of diffuse large B-cell lymphoma. Proc Natl Acad Sci U S A 114:11404-11409 (2017). PubMed: 29073064
